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  • Springer  (84)
  • EDP Sciences  (6)
  • American Physical Society (APS)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 52 (1965), S. 345-346 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 52 (1965), S. 166-167 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' journal of analytical chemistry 357 (1997), S. 355-356 
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A new procedure for the field characterization of the available amount of molybdenum in soil extract is described. It is based on the preconcentration of the Mo(V)-SCN– complex into toluene solution of N-octylacetamide (OAA) and subsequent heating of the extract for ≈ 10 min at 60° C over a water bath. The molar absorptivity of the complex is (3.50) × 104 1 mole–1 cm–1 at λmax 470 nm. The detection limit of the method is 4 ppb Mo. It provides a significantly increased tolerance limit for iron (up to 1000 ppm) and is applicable to the field characterization of Mo in soil extracts.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' journal of analytical chemistry 360 (1998), S. 270-272 
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A new, simple, selective and sensitive method for the spectrophotometric determination of antimony in rain water is described. It includes preconcentrating Sb with surfactants (i.e. cetylpyridinium chloride (CPC) and Triton X-100 (TX-100)) into toluene and allowing the extract to react with a dye, i.e. brilliant green (BG). The value of apparent molar absorptivity is 5.55 × 105 L-mol–1· cm–1 at λmax = 620 nm; the detection limit is 3 ng/mL Sb in rain water at 3-fold preconcentration.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' journal of analytical chemistry 367 (2000), S. 738-741 
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A new and sensitive procedure for the graphite furnace-atomic absorption spectrophotometric (GF-AAS) determination of Pd in soil at nanogram level is described. The method is based on prior separation and enrichment of the metal as Pd(II)-SnCl3 –-N-butylacetamide (BAA) complex into 1-pentanol (PN) by solvent extraction method. The value of the molar absorptivity of the complex in three solvents, i.e. ethyl acetate, 1-pentanol, chloroform, lie in the range of (0.70–2.75) × 104 L mol–1 cm–1 at λmax 360–440 nm. The metal could be enriched into organic solvent, i.e. PN, up to 10-folds. The sensitivity (A = 0.0044) of the method in the term of the peak height was 0.5 ng Pd/mL of the aqueous solution at an enrichment factor (EF) of 5. Optimization of analytical variables during enrichment and GF-AAS determination of the metal are discussed. The method has been applied for the analysis of Pd to soil samples derived from roads and highways in Germany.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 69 (1980), S. 101-109 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cytochemical detection of ascorbic acid in cultured root tips of Zea mays shows that dividing cells accumulate ascorbic acid in the cytoplasm. The localization pattern alters in the root tip as the cells begin to elongate. In elongating cells ascorbic acid is distinctly localized on cell walls. Ascorbic acid content per cell inreases with the onset of cell elongation. Fully elongated cells contain fivefold more ascorbic acid than meristematic cells. Cytophotometric analysis reveals a sharp and positive correlation (r=+0.93) between percentage increase in content of ascorbic acid per cell and corresponding increase in cell size at different phases of cell elongation. IAA treatment to the roots raises the content of ascorbic acid per cell with a parallel increase in size of cell. Involvement of ascorbic acid in IAA induced cell elongation is discussed.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 102 (1994), S. 383-387 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Detection of DNA synthesizing cells may often be achieved by immunocytochemical detection of bromodeoxyuridine (BrdU), which is rapid and appears to give similar results to those found using tritiated thymidine. However, the methodology for detection of BrdU involves a denaturation or digestion step to allow access of the antibody to BrdU incorporated into single- rather than double-stranded DNA. We wished to determine if microwave treatment could be used to enhance the detection of BrdU without the need for any other digestion/denaturation steps. An important consideration was to investigate whether such treatment produces a similar quantitative result, since BrdU detection is usually assessed on the basis of cell number rather than topographical distribution. We have found that microwave treatment can allow considerably lower antibody concentrations and eliminates the need for any other denaturation step. It also reduces the non-specific background staining found when using monoclonal antibodies on mouse tissue. We have performed cell counts and found that the number of BrdU positive cells remains constant for a range of different immunocytochemical parameters. We also report conditions where immunopositivity is adversely affected by changes in technique and describe the optimised conditions for obtaining reproducible results.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' journal of analytical chemistry 362 (1998), S. 571-576 
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A new, simple, rapid and selective flow injection analysis (FIA) method for the spectrophotometric quantification (speciation of inorganic and organic form) of Zn and Co with ammonium thiocyanate and malachite green (MG) in the presence of surfactants (CPC and TX-100) is described. The value of apparent molar absorptivity of the Zn- and Co- complexes are (1.23) × 104 and (8.67) × 103 L mol–1cm–1 at absorption maximum, 635 nm, respectively. The detection limit (amount causing a peak height 〉 3 s) is 15 ppb Zn and 20 ppb Co, whereas their optimum working ranges for the quantitative determinations are 0.05–2.0 ppm Zn and 0.07–2.5 ppm Co in the real samples. The sample thoughput of the method is 120 samples/h at the flow rate of 5.0 mL/min with rel. std. dev. of 〈 ± 1%. The method is free from interferences of almost all ions which are commonly associated with these metals in the complex materials. The composition of the complexes and their reaction mechanism involved are discussed. The effect of FIA and analytical variables for the determination of the metals are optimized. The method has been applied to the quantification of Zn and Co in beverages, biological, environmental, and pharmaceutical samples.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Colloid & polymer science 265 (1987), S. 592-596 
    ISSN: 1435-1536
    Keywords: Morphology ; crystallization ; singlecrystal ; trans-1 ; 4-polyisoprene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The film formation method has been successfully used to grow single crystals and other complex morphological features of low melting form (LMF) and high melting form (HMF) of trans-1,4-polyisoprene (TPIP). Below 40 °C dilute amyl acetate solution gave hexagonal shaped LMF crystals. Thick and elongated hexagonal shaped morphology was shown by HMF crystals at temperatures above 40 °C. Straight faces and sharp corners of the single crystals, and also of complex crystals, acquired round shapes when highly polydispersed TPIP was used for crystallization.
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  • 10
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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