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  • 1
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    γ -ray spectroscopy study of 11 ΛB and 12 ΛC (2007)
    Springer
    Details
    Publication Date: 2007-09-01
    Print ISSN: 1434-6001
    Electronic ISSN: 1434-601X
    Topics: Physics
    Published by Springer
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    PAPER CURRENT
  • 2
    Electronic Resource
    Electronic Resource
    Two sulfur-containing ansamycin antibiotics fromStreptomyces albolongus (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 1167-1170 
    Details
    ISSN: 1420-9071
    Keywords: Ansamycin ; ansathiazin ; awamycin ; Streptomyces albolongus C-46366 ; gram-positive bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two sulfur-containing ansamycin antibiotics were isolated from the culture broth ofStreptomyces albolongus C-46366; the major one was identical with awamycin and the minor one was a new ansamycin antibiotic, ansathiazin. Their structures were elucidated from their reactions and spectroscopic analyses. These antibiotics were active against gram-positive bacteria, acid-fast bacteria and a protozoan.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01941296
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  • 3
    Electronic Resource
    Electronic Resource
    The Basis of Higher Na+ Transport by Inner Medullary Collecting Duct Cells from Dahl Salt-Sensitive Rats: Implicating the Apical Membrane Na+ Channel (1997)
    Springer
    The journal of membrane biology 156 (1997), S. 9 -18 
    Details
    ISSN: 1432-1424
    Keywords: Key words: Na+ transport — Salt-sensitive rats — Apical membrane — Na/K pumps — IMED cells — Kidney cortex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. The present experiments were designed to examine the function of Na/K pumps from Dahl salt-sensitive (S) and salt-resistant (R) rats. Previous reports have suggested that there is a difference in primary sequence in the α1 subunit, the major Na/K pump isoform in the kidney. This sequence difference might contribute to differences in NaCl excretion in these two strains which in turn could influence the systemic blood pressure. Using ``back-door'' phosphorylation of pumps isolated from basolateral membranes of kidney cortex, we found no differences between S and R strains. We also examined the Na/K pumps from cultured inner medullary collecting duct (IMCD) cells. This approach takes advantage of the fact that monolayers cultured from S rats transport about twice as much Na+ as monolayers cultured from R rats. In cells whose apical membrane was made permeable with amphotericin B, comparison of the affinities for ouabain, Na+, and K+, respectively, showed only small or no differences between S and R monolayers. Ouabain binding showed no difference in the number of Na/K pumps on the basolateral membrane of cultured cells, despite a 2-fold difference in Na+ transport rates. The analysis of the steady-state Na+ transport indicates that Na/K pumps in IMCD monolayers from S rats operate at a higher fraction of their maximum capacity than do pumps in monolayers from R rats. The results, taken together, suggest that the major reason for the higher rate of Na+ transport in S monolayers is because of a primary increase in the conductive permeability of the apical membrane to Na+. They suggest that the epithelial Na+ channel is intrinsically different or differently regulated in S and R rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002329900182
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  • 4
    Electronic Resource
    Electronic Resource
    Cloning and expression of the chicken immunoglobulin joining (J)-chain cDNA (2000)
    Springer
    Immunogenetics 51 (2000), S. 85-91 
    Details
    ISSN: 1432-1211
    Keywords: Key words J chain ; Polymeric immunoglobulin ; Ontogeny ; Evolution ; Comparative immunology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The J chain is a component of polymeric immunoglobulin (Ig) molecules and may play an important role in their polymerization and the transport of polymeric Ig across epithelial cells. In this study, the primary structure of the chicken J chain was determined by sequencing cDNA clones. The cDNA had an open reading frame of 476 nucleotides encoding a putative protein of 158 amino acid residues including the signal sequence. The 3′ untranslated region consisted of 1216 nucleotides and a poly(A) tail. The deduced amino acid sequence of the chicken J chain had a high degree of homology to that of human, cow, rabbit, mouse, frog, and earthworm, with eight conserved Cys residues identical to the mammalian J chains. Northern blot hybridization performed with total RNA from various chicken tissues revealed high levels of J-chain mRNA expression in spleen, intestine, Harderian gland, and bursa of Fabricius, and low levels in the thymus. The J chain was expressed in the bursa as early as day 15 of embryogenesis. These data indicated that the chicken J-chain gene displays a high degree of homology with that of other species, and is expressed at an early stage of development of the chicken immune system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050016
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  • 5
    Electronic Resource
    Electronic Resource
    Role of nuclear factor-κ B in the expression by tumor necrosis factor-α of the human polymeric immunoglobulin receptor (pIgR ) gene (2000)
    Springer
    Immunogenetics 51 (2000), S. 289-295 
    Details
    ISSN: 1432-1211
    Keywords: Key words Human ; Mucosa ; Gene regulation ; Cytokines ; Transcription factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  We analyzed the mechanism of human polymeric immunoglobulin receptor (pIgR) gene upregulation by tumor necrosis factor (TNF)-α. Northern blot analysis showed that the expression of pIgR mRNA was enhanced by TNF-α stimulation. This activation was completely inhibited by RNA polymerase or protein synthesis inhibitors, suggesting that the regulation of pIgR gene expression depends on de novo RNA and protein synthesis. Furthermore, the stimulation of pIgR mRNA by TNF-α was decreased by pyrrolidinedithiocarbamate and l-1-4′-tosylamino-phenylethyl-chloromethyl ketone, which are known nuclear factor (NF)-κB inhibitors. For further analysis of gene regulation, we cloned and sequenced the 1.5-kb 5′-flanking region of the pIgR gene. In the upstream region, we found two NF-κB-binding motifs (named κB1 and κB2 from the 5′ region). An electrophoretic mobility shift assay indicated that two components of the NF-κB/Rel family, p50 and p65, bound with higher affinity to the κB2 element than to the κB1 element. We also analyzed pIgR gene expression using reporter plasmids expressing the firefly luciferase gene. Stimulation by TNF-α significantly activated the pIgR gene promoter, as a 775-bp upstream region of the pIgR gene increased luciferase gene expression in cells treated with TNF-α. The activation of promoter activity by TNF-α was abolished when a mutation was inserted into κB1 or κB2. These data indicated that pIgR gene expression induced by TNF-α is transcriptionally regulated via activation of NF-κB. In addition, there is a possibility that another factor may act in concert with NF-κB.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050622
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  • 6
    Electronic Resource
    Electronic Resource
    Measurement of flame temperature by optical fiber thermometer (1988)
    Springer
    Experiments in fluids 6 (1988), S. 514-520 
    Details
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The characteristics of an optical fiber thermometer (OFT) having a black body cavity sensor, constructed with a thin platinum film sputtered on a sapphire rod tip, were examined and compared with those of bare or sheathed thermocouples. For time averaged temperature measurements by the OFT, the radiative heat loss is significant in the temperature range of flames. For fluctuating temperatures the OFT output has a slow response, due to its thermal inertia, which depends on the diameter of the sensor and flow conditions; in spite this the rapid response of the OFT is emphasized in the literature with the assumption of the ideal thermal behavior of the thin sputtered film ( ⩰ 4 μm) on a sapphire rod of 0.25 mm diameter. The fluctuation waveform predicted by the present numerical simulation agrees satisfactorily with the measured one, which proves that the major factor affecting the thermal inertia of an OFT sensor is the heat capacity of sapphire. A special compensation technique is needed to improve the response of the OFT.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00196597
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  • 7
    Electronic Resource
    Electronic Resource
    Simulation study on dynamics transition in neuronal activity during sleep cycle by using asynchronous and symmetry neural network model (1990)
    Springer
    Biological cybernetics 63 (1990), S. 243-250 
    Details
    ISSN: 1432-0770
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Computer Science , Physics
    Notes: Abstract We have found that single neuronal activities in different regions in the brain commonly exhibit the distinct dynamics transition during sleep-waking cycle in cats. Especially, power spectral densities of single neuronal activities change their profiles from the white to the 1/f along with sleep cycle from slow wave sleep (SWS) to paradoxical sleep (PS). Each region has different neural network structure and physiological function. This suggests a globally working mechanism may be underlying the dynamics transition we concern. Pharmacological studies have shown that a change in a wide-spread serotonergic input to these regions possibly causes the neuronal dynamics transition during sleep cycle. In this paper, based on these experimental results, an asynchronous and symmetry neural network model including inhibitory input, which represents the role of the serotonergic system, is utilized to examine the reality of our idea that the inhibitory input level varying during sleep cycle induce that transition. Simulation results show that the globally applied inhibitory input can control the dynamics of single neuronal state evolution in the artificial neural network: 1/f-like power spectral density profiles result under weak inhibition, which possibly corresponds to PS, and white profiles under strong inhibition, which possibly corresponds to SWS. An asynchronous neural network is known to change its state according to its energy function. The geometrical structure of network energy function is thought to vary along with the change in inhibitory level, which is expected to cause the dynamics transition of neuronal state evolution in the network model. These simulation results support the possibility that the serotonergic system is essential for the dynamics transition of single neuronal activities during sleep cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00203447
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  • 8
    Electronic Resource
    Electronic Resource
    Mapping the fermi surface of graphite with a display-type photoelectron spectrometer (1991)
    Springer
    Applied physics 52 (1991), S. 299-301 
    Details
    ISSN: 1432-0630
    Keywords: 79.60.Cn ; 71.25.Pi ; 71.25.Hc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A display spectrometer is used to image the momentum distribution of photoelectrons from the Fermi level in graphite. The Fermi “surface” consists of six points at the corners K of the hexagonal, two-dimensional Brillouin zone, in agreement with band theory. The method is also applied to other equal energy surfaces below the Fermi level, thereby giving the band dispersion of the π-bands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00324768
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  • 9
    Electronic Resource
    Electronic Resource
    The measurement of exonuclease activities by atomic force microscopy (1998)
    Springer
    European biophysics journal 27 (1998), S. 63-68 
    Details
    ISSN: 1432-1017
    Keywords: Key words DNA ; Enzyme activity ; Atomic force microscopy ; Exonuclease ; BAL 31 nuclease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract We have applied atomic force microscopy (AFM) to the measurement of BAL 31 nuclease activities. BAL 31 nuclease, a species of exonuclease, is used to remove unwanted sequences from the termini of DNA before cloning. For cutting out only the appropriate sequences, it is important to know the nuclease properties, such as digestion speed and the distribution of the lengths of the digested DNA. AFM was used to obtain accurate measurements on the lengths of DNA fragments before and after BAL 31 nuclease digestion. We analyzed 4 DNAs with known number of base pairs (288, 778, 1818, and 3162 base pairs) for correlating the contour length measured by AFM with the number of base pairs under the deposition conditions used. We used this calibration for analyzing DNA degradation by BAL 31 nuclease from the AFM measurement of contour lengths of digested DNAs. In addition, the distribution of digested DNA could be analyzed in more detail by AFM than by electrophoresis, because digested DNA were measured as a population by electrophoresis, but were measured individually by AFM. These results show that AFM will be a useful new technique for measuring nuclease activities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002490050111
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  • 10
    Electronic Resource
    Electronic Resource
    Selective preparation of copper, copper(I) oxide, or copper(II) oxide fine particles from organocopper compounds (1988)
    Springer
    Journal of materials science 7 (1988), S. 1251-1252 
    Details
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00722355
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