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  • 1
    Electronic Resource
    Electronic Resource
    The effect of ethanol on lateral and rotational mobility of plasma membrane vesicles isolated from cultured mar 18.5 hybridoma cells (1994)
    Springer
    The journal of membrane biology 138 (1994), S. 221-227 
    Details
    ISSN: 1432-1424
    Keywords: Ethanol ; Hybridoma cell plasma membrane vesicles ; Transbilayer fluidity asymmetry ; Fluorescent probe technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Intramolecular excimer formation of 1,3-di(1-pyrenyl)propane (Py-3-Py) and fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) were used to evaluate the effect of ethanol on the rate and range of the lateral mobility and the range of the rotational mobility of bulk bilayer structures of the plasma membrane vesicles (ATCC-PMV) isolated from cultured hybridoma cells (ATCC TIB 216). In a concentration-dependent manner, ethanol increased the excimer to monomer fluorescence intensity ratio (I′/I) of Py-3-Py in the ATCC-PMV and decreased the anisotropy (r), limiting anisotropy (r∞) and order parameter (S) of DPH in the ATCC-PMV. This indicates that ethanol increased both the lateral and rotational mobility of the probes in the ATCC-PMV. Selective quenching of DPH by trinitrophenyl groups was utilized to examine the range of transbilayer asymmetric rotational diffusion of the ATCC-PMV. The anisotropy (r), limiting anisotropy (r ∞) and order parameter (S) of DPH in the inner monolayer were 0.024, 0.032, and 0.069, respectively, greater than calculated for the outer monolayer of the ATCC-PMV. Selective quenching of DPH by trinitrophenyl groups was also used to examine the transbilayer asymmetric effects of ethanol on the range of the rotational mobility of the ATCC-PMV. Ethanol had a greater increasing effect on the range of the rotational mobility of the outer monolayer as compared to the inner monolayer of the ATCC-PMV. It has been proven that ethanol exhibits a selective rather than nonselective fluidizing effect within the transbilayer domains of the ATCC-PMV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232794
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of K+ Channels in the Basolateral Membrane of Rat Tracheal Epithelia (1996)
    Springer
    The journal of membrane biology 154 (1996), S. 251 -257 
    Details
    ISSN: 1432-1424
    Keywords: Key words: Trachea — K+ channels — Basolateral membrane — cAMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. To study K+ channels in the basolateral membrane of chloride-secreting epithelia, rat tracheal epithelial monolayers were cultured on permeable filters and mounted into an Ussing chamber system. The mucosal membrane was permeabilized with nystatin (180 μg/ml) in the symmetrical high K+ (145 mm) Ringer solution. During measurement of the macroscopic K+ conductance properties of the basolateral membrane under a transepithelial voltage clamp, we detected at least two types of K+ currents: one is an inwardly rectifying K+ current and the other is a slowly activating outwardly rectifying K+ current. The inwardly rectifying K+ current is inhibited by Ba2+. The slowly activating K+ current was potentiated by cAMP and inhibited by clofilium, phorbol 12-myristae 13-acetate (PMA) and lowering temperature. This is consistent with the biophysical characteristics of I SK channel. RT-PCR analysis revealed the presence of I SK cDNA in the rat trachea epithelia. Although 0.1 mm Ba2+ only had minimal affect on short-circuit current (I sc) induced by cAMP in intact epithelia, 0.1 mm clofilium strongly inhibited it. These results indicate that I SK might be important for maintaining cAMP-induced chloride secretion in the rat trachea epithelia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002329900149
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  • 3
    Electronic Resource
    Electronic Resource
    A coherent optical feedback system for optical information processing (1975)
    Springer
    Applied physics 8 (1975), S. 51-58 
    Details
    ISSN: 1432-0630
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A unique optical feedback system for coherent optical data processing is described. With the introduction of feedback, the well-known transfer function for feedback systems is obtained in two dimensions. Operational details of the optical feedback system are given. Experimental results of system applications in image restoration, contrast control and analog computation are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00883670
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  • 4
    Electronic Resource
    Electronic Resource
    The tumor promoter pristane activates transcription by a cAMP dependent mechanism (1992)
    Springer
    Molecular and cellular biochemistry 110 (1992), S. 75-81 
    Details
    ISSN: 1573-4919
    Keywords: pristane ; tumor promoter ; cAMP response element
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Pristane is a naturally occurring isoprenoid which is believed to be derived from the phytyl moiety of chlorophyll. Thus it is not surprising that pristane is present in many common fruits or vegetables and furthermore can be detected in tissues of fish and mammals. Using the rat as an animal model, pristane can function as a potent tumor promoter. It is conceivable that pristane could play a role in the development of certain malignancies in higher mammals since it is commonly found in the diet. At the molecular level, pristane can induce changes in the plasma membrane, alter the conformation of chromatin, as well as selectively activate gene expression. This study was undertaken to identify specific transcriptional motifs which are responsive to pristane. A transcriptional promoter which contained a cAMP response element (CRE) was consistently stimulated by pristane in several mouse and primate cell lines. A promoter construct which contained a single copy of the TPA response element (TRE) was also activated by pristane but surprisingly a promoter which contained multiple copies of the TRE was not. Activation of the TRE required 10 fold higher concentrations of pristane relative to activation of the CRE. Within two hours after addition of pristane to monkey fibroblasts (CV-1) levels of cAMP were increased more than two fold relative to controls. These data indicated that pristane can increase the level of cAMP in CV-1 cells and consequently stimulate transcriptional promoters which contain a CRE.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02385008
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  • 5
    Electronic Resource
    Electronic Resource
    Gradual downslope change in high-resolution acoustic characters and geometry of large-scale submarine debris lobes in Ulleung Basin, East Sea (Sea of Japan), Korea (1999)
    Springer
    Geo-marine letters 19 (1999), S. 254-261 
    Details
    ISSN: 1432-1157
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract A detailed analysis of high-resolution seismic data (Chirp, 2–7 kHz) in the Ulleung Basin reveals spatial variation in echo characteristics and geometry of large-scale debris lobes. In the proximal part, the debris lobes are dominated by hummocky surface echoes and gradually transitional downslope to seafloor-tangent hyperbolic and smooth prolonged bottom echoes, reflecting progressive decrease in size, spacing, and relief of surface forms. The strongly convex-upward upper surface with steep blunt margins in the proximal part is gradually transitional downslope to slightly convex- upward or nearly flat form with less blunt margins. The gradual downslope decrease in both scale and spacing of surface forms and convexity of upper surface within each debris lobe is suggestive of progressive dilution in flow concentration within a debris flow, probably due to mixing of ambient water and removal of suspended sediment by deposition during downslope movement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003670050116
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  • 6
    Electronic Resource
    Electronic Resource
    WAPK, a Ser/Thr protein kinase gene of Nicotiana tabacum, is uniquely regulated by wounding, abscisic acid and methyl jasmonate (1998)
    Springer
    Molecular genetics and genomics 259 (1998), S. 516-522 
    Details
    ISSN: 1617-4623
    Keywords: Key words Protein kinase ; Wounding ; Abscisic acid ; Methyl jasmonate ; Signal transduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA encoding a protein kinase, which may be involved in the wound signal transduction pathway, was isolated from Nicotiana tabacum. The cDNA, named WAPK, is 1227 bp in length and contains an ORF of 1017 bp. The ORF encodes a polypeptide of 339 amino acids, with a calculated molecular mass of 38234 Da. Analysis of the deduced amino acid sequence shows that the N-terminal region of WAPK contains a catalytic region composed of eleven subdomains which are typically found in Ser/Thr protein kinases. This region shows 78–84% sequence identity with similar regions of abscisic acid (ABA)-induced and external-stimuli-responsive protein kinases. However, the C-terminal region of WAPK shows little homology with similar regions of Ser/Thr protein kinases, except for a 16-amino acid stretch near the end of the catalytic domain. Kinase assays using a WAPK fusion protein expressed in E. coli revealed that WAPK autophosphorylates on serine residue(s). The WAPK gene is predominantly expressed in flowers, moderately in roots, and poorly in leaves. Transcripts were not detected in stems. The WAPK gene was induced by wounding (within 1.5 h), by abscisic acid (within 0.5 h), and by methyl jasmonate (within 2 h). The induction pattern of WAPK mRNA upon wounding was not affected by treatment with diethyldithiocarbamic acid, a reagent which inhibits jasmonic acid biosynthesis. These results suggest that the WAPK gene is regulated by ABA in the wound signal transduction pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050843
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  • 7
    Electronic Resource
    Electronic Resource
    Transient effect on the constriction resistance between spheres (2000)
    Springer
    Computational mechanics 25 (2000), S. 59-65 
    Details
    ISSN: 1432-0924
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  Systems of contacting spheres are common in engineering applications where the heat transfer analysis can be quite cumbersome due to the transient behavior and the complex geometrical arrangement. As a result, most of the previous works, in this area, have adopted the porous media approach. However, this approach requires the length scale of the representative cell to be roughly three orders of magnitude larger than the size of the spheres. Constriction resistance relations could be useful in accurately computing the temperature distribution within systems of contacting spheres, however many of the requisite relations are not available. Thus, the objective of this study is to develop these relations. In this study, the transient, three-dimensional conduction equation was solved using a finite volume scheme and a non-uniform grid. From the resulting temperature distributions, the steady-state and transient constriction resistance of one-sphere and two-sphere systems were computed and correlated. The results also showed, for the first time, the critical parameters below which the transient variations must be considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004660050015
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  • 8
    Electronic Resource
    Electronic Resource
    An improved procedure for plant regeneration from indica and japonica rice protoplasts (1995)
    Springer
    Plant cell reports 14 (1995), S. 515-519 
    Details
    ISSN: 1432-203X
    Keywords: Oryza sativa L. ; indica ; japonica ; protoplast culture ; feeder cells ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plant regeneration from protoplasts of two commercially cultivated Indian indica rice varieties, Pusa Basmati 1 and Java, has been accomplished by plating embryogenic cell suspension-derived protoplasts on the surface of filter membranes overlying agarose-embedded feeder cells of Lolium multltiflorum and Oryza ridleyi, combined with the use of a maltose-containing shoot regeneration medium. Embryogenic cell suspension cultures of Pusa Basmati 1 and Jaya were initiated from mature seed scutellum-derived calli in liquid R2 medium modified by the addition of 560 mg l−1 of proline and 1.0 % (w/v) maltose. In both varieties, protoplast plating efficiencies up to 0.4 % were obtained, depending on the nature of the feeder cells. L. multiflorum feeder cells induced a 6-fold higher plating efficiency than feeder cells of O. ridleyi. In combination, O. ridleyi and L. multiflorum feedercells further enhanced protoplast plating efficiency. Protoplast-derived cell colonies were not obtained from protoplasts of either indica varieties in the absence of feeder cells. MS-based medium containing kinetin (2.0 mg l−1) and α-naphthaleneacetic acid (0.5 mg 1−1), together with sucrose and maltose both at 1.5 % (w/v), induced green shoot regeneration in 44 % of protoplast-derived tissues, depending on the feeder cells used for protoplast culture. In both varieties, tissues obtained using O. ridleyi feeder cells were more morphogenic than tissues obtained using L. multiflorum feeder cells, either alone or in combination with cells of O. ridleyi. In the japonica rice variety Taipei 309, this new procedure resulted in a 30-fold increase in plant regeneration from protoplasts compared to previous published procedures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232786
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  • 9
    Electronic Resource
    Electronic Resource
    Aldehyde as fixative for histochemical study of glutamic oxaloacetic transaminase (1968)
    Springer
    Histochemistry and cell biology 12 (1968), S. 341-344 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Several compounds of the aldehyde group have been tested as fixatives for histochemical localization of GOT activity in the rat kidney. A brief fixation of cryostat tissue sections in 1% glutaraldehyde results in well localized enzymatic activity which is not greatly further affected by an additional treatment in 4% formaldehyde.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00278306
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  • 10
    Electronic Resource
    Electronic Resource
    Uterine epithelial and eosinophil estrogen receptors in rats during the estrous cycle (1982)
    Springer
    Histochemistry and cell biology 74 (1982), S. 443-452 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the uterus of the adult female rats, the luminal epithelial cells and the eosinophil leukocytes are rich in cytoplasmic estrogen receptors. During the estrous cycle, the epithelial estrogen receptor concentration reaches its peak level, in proestrus, drops precipitously in estrus, and hits the trough, at metestrus. Repopulation of the cytoplasm with estrogen binding sites occurs during diestrus. This pattern of cyclic change is indicative of a rapid turnover of estrogen receptors in the epithelial cells and its regulation by endogenous estrogens. The concentration of estrogen receptors in the cytoplasm of the eosinophils does not appear to fluctuate during the cycle. But the intrauterine, distribution of these leukocytes is clearly cyclic in pattern, ostensibly influenced by estrogens. While progesterone binding activity is consistently demonstrated in tandem with estrogen receptors in the cytoplasm of the epithelial cells, it has not been observed in the eosinophil leukocytes. These findings support the claim that there are two estrogen receptor systems in the rat uterus, one mediating the intracellular events of the genomic response to estrogens, and the other being concerned with non-genomic responses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00496657
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