ISSN:
1432-1327
Schlagwort(e):
Key words Quinolones
;
Chemical nucleases
;
DNA-interacting compounds
;
SOB response
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
Abstract Alteration of bacterial DNA structure and/or associated functions in vivo by [Cu(phen)(nal)]+, a metal complex of the type [Cu(phen)(antib)]+ (where antib is a quinolone or a fluoroquinolone), was demonstrated by the induction of a recA-lacZ fusion integrated at the amyE locus of a recombinant Bacillus subtilis strain. Using the same approach, nalidixic acid alone was shown to induce 14% of the β-galactosidase levels induced by [Cu(phen)(nal)]+; on the other hand none of the other components, i.e. copper, phenanthroline or the complex [Cu(phen)2]2+ activated significantly the recA-directed β-galactosidase activity, suggesting that the intact structure of the complex is required to reach maximum levels of induction. Results of in vitro experiments demonstrated that under reductive conditions [Cu(phen)(nal)]+ behaves as a powerful nuclease capable of degrading plasmid DNA; this activity was stronger than that of the chemical nuclease copper phenanthroline [Cu(phen)2]2+. The nuclease activity putatively occurred by a mechanism involving hydroxyl radicals since the reaction was partially inhibited by catalase. These results support the hypothesis that the mechanism of action of quinolones could be mediated by a transition metal ion such as copper.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s007750050219
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