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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 18 (1904), S. 253-260 
    ISSN: 1432-041X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Aldehydes, aliphatic ; 1,3-Cyclohexanedione (CHD) ; Fluorimetric detection ; Plasma analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A simple and sensitive method is proposed for the measurement of aldehydes in plasma. The plasma samples are treated with 1,3-cyclohexanedione (CHD) and after addition of the internal standard (the CHD derivative of octaldehyde) are analysed by reversed-phase high-performance liquid chromatography with an acetonitrile-water gradient as the mobile phase. Detection is by fluorimetry with excitation at 395 nm and emission at 457 nm. In plasma samples spiked with aldehydes, the calibration curves showed good linearity in the range 0–10 μg mL−1. The recoveries of aldehydes were quantitative; coefficients of within-day and between-day variation were less than 13% and 11%, respectively. Applications to human and some animal plasma samples are reported.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 262 (1999), S. 131-138 
    ISSN: 1617-4623
    Keywords: Key wordsEyes absent ; Organ morphogenesis ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Homologs of the eyes absent (eya) gene in animals function at multiple stages in the development of organs. Their functional roles in the genetic network that regulates eye development in Drosophila have recently been extensively analyzed. A rice homolog of eya was identified from a cDNA library made from embryo RNA. The corresponding gene (OSEya1) encodes a conserved ED1 domain and a short N-terminal peptide. The ED1 domain of OSEya1 shows 25% identity and 36% similarity to the product of Drosophila eya. Mammalian and squid eya homologs show about 35% similarity to OSEya1. Homologous sequences were also found in the alfalfa EST database (53% identity and 65% similarity to OSEya1) and in the Arabidopsis genome sequence (63% identity). Therefore, eya homologs are present in both monocots and dicots. Three regions in the ED1 domain are well conserved in animals and plants. Plant eya products deduced from the nucleotide sequences also have short N-terminal peptides. The OSEya1 gene is located between the wx gene and the telomere on the short arm of chromosome 6. OSEya1 is expressed in the embryo, shoot apex, and caryopsis in rice. Expression in the embryo increases during embryogenesis until 7 days after pollination, with preferential localization in leaf primordia and the shoot apical meristem. Expression in the influorescence was observed in floral meristems. The functions of OSEya1 in higher plants are discussed and compared with those of their animal homologs. OSEya1 might regulate the morphogenesis of lateral organs as a subunit of a transcription factor.
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  • 4
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Urinary melatonin excretion is lower in East-Asian (Japanese) than in North-American (whites of mixed ethnic origin) women. Moreover, a statistically significant circadian rhythm is demonstrated by population-mean cosinor in the data pool from both groups of women. Furthermore, statistical significance characterizes interactions of effects from geographic differences (between ethnic groups) with temporal factors. Such spatio-temporal interactions await further scrutiny with a view inter alia of carcinogenesis as it is influenced by a spectrum of intermodulating rhythms.
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  • 5
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Der Hypothalamus von Kaninchen wurde nach Win 18501-2-Injektion biochemisch und elektronenmikroskopisch untersucht. Die Behandlung erzeugte auffallende Veränderungen im Noradrenalingehalt und in der Anzahl der Katecholamine enthaltenden granulierenden Vesiculae im Hypothalamus. Die Resultate scheinen zu zeigen, dass diese charakteristischen Vesiculae nur Noradrenalin enthalten und dass die Sedation des Kaninchens nach der Injektion von Win 18501-2 auf der Verminderung des Noradrenalins und der charakteristischen Vesiculae beruht.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-203X
    Keywords: Key wordsBrassica napus ; Microspore culture ; Transgenic plant ; Particle bombardment ; Doubled haploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A procedure for direct gene transfer into isolated microspores of rapeseed (Brassica napus L.) and the production of fertile transgenic plants is presented. By modifying the microspore culture method and adopting the firefly luciferase (Luc) gene as a non-destructive marker, we could obtain stably transformed androgenetic embryos from bombarded microspores. Luc-positive embryos were easily isolated from the large non-transformed population using a high-sensitivity bioluminescent image analyzer. PCR and Southern blot analyses confirmed that the introduced transgene was integrated stably into the genome of the selected embryos. Diploidized plants obtained from the haploid embryos were self-pollinated, and all of the offspring tested were Luc-positive, indicating rapid fixation of the transgene which is characteristic of doubled haploids.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 22 (1980), S. 433-437 
    ISSN: 1432-0630
    Keywords: 07.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Generation of backward photon echoes and proposed backward optical free induction decays is discussed in detail. Separation of the signals from excitation pulses by propagation and polarization directions suggests a new field of picosecond experiments.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 55 (1992), S. 233-241 
    ISSN: 1432-0649
    Keywords: 42.50.Dv ; 42.50.Lc ; 42.65.Ky
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Broadband high level squeezing was clearly observed from 100 kHz to 80 MHz using crystals Ba2NaNb5O15 of 5 mm length, MgO:LiNbO3 of 19 mm length and KNbO3 of 5.8 mm length. Maximum noise reductions detected on a spectrum analyzer were −1.2 dB (−24%), −1.25 dB (−25%), and −1.8 dB (−34%) for the three crystals, respectively. The maximum squeezing is limited mostly by “optical index damage” of the parametric crystals. A detailed analysis of the beam parameters traced along the pump beam, squeezed vacuum, etc. is given. A detailed discussion on the evaluation of the initial squeezed level is given. A preliminary experiment with compressed laser pulses to avoid the optical damage is also described.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Astrophysics and space science 4 (1969), S. 44-63 
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The intensity of the diffuse component of cosmic X-rays was measured with use of a rotating collimator system borne on a sounding rocket. A part of background counts proportional to the field of view of proportional counters enabled us to determine the intensity of the diffuse component to be 0.66±0.07 photons cm−2 sec−1 keV−1 in the energy range between 3.6 and 9.0 keV. The spectrum in this energy range was found to be comparatively flat. The intensity of Sco X-1 was also measured and its time variation was investigated.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Astrophysics and space science 42 (1976), S. 211-216 
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract In the record of the balloon observation which was performed on 27 September, 1970, a transient burst of X-rays was found. This event is concluded to be a cosmic gamma-ray burst of a smaller size or of a larger distance compared to the Vela bursts observed over the X-ray energy range. The energy spectrum is consistent with that of some of the Vela bursts. The time profile of the event is qualitatively similar to the 27 April, 1972 event studied by Apollo 16. The detection of small bursts over the X-ray energy range by the balloon observation during a period of the order of 10 to 100 h is not surprising considering a probable frequency-size distribution of the burst.
    Type of Medium: Electronic Resource
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