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  • Springer  (617)
  • American Association for the Advancement of Science  (13)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Marine biology 89 (1985), S. 213-219 
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This study describes some aspects of the distribution, ecology, and rock boring behaviour of the shrimp Alpheus saxidomus Holthuis from the Pacific coast of Costa Rica. This species has a typical hammer-shaped dactylus on the first right pereiopod of females and males that shows tracks of abrasion at the hammer-head. Structural analysis of this hammer-dactylus proved that it is suited for the mechanical treatment of rocks. The very thick and calcified cuticle of the hammer is mostly formed by the sclerotinized, hard exocuticle, which has abundant pore canals.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 160 (1993), S. 440-446 
    ISSN: 1432-072X
    Keywords: Archaebacteria ; Halophile ; Methanogen ; Methanohalophilus mahii ; Aromatic amino acid biosynthesis ; Metabolic interlock
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Extensive diversity in features of aromatic amino acid biosynthesis and regulation has become recognized in eubacteria, but almost nothing is known about the extent to which such diversity exists within the archaebacteria. Methanohalophilus mahii, a methylotrophic halophilic methanogen, was found to synthesize l-phenylalanine and l-tyrosine via phenylpyruvate and 4-hydroxyphenylpyruvate, respectively. Enzymes capable of using l-arogenate as substrate were not found. Prephenate dehydrogenase was highly sensitive to feedback inhibition by l-tyrosine and could utilize either NADP+ (preferred) or NAD+ as cosubstrate. Tyrosine-pathway dehydrogenases having the combination of narrow specificity for a cyclohexadienyl substrate but broad specificity for pyridine nucleotide cofactor have not been described before. The chorismate mutase enzyme found is a member of a class which is insensitive to allosteric control. The most noteworthy character state was prephenate dehydratase which proved to be subject to multimetabolite control by feedback inhibitor (l-phenylalanine) and allosteric activators (l-tyrosine, l-tryptophan, l-leucine, l-methionine and l-isoleucine). This interlock type of prephenate dehydratase, also known to be broadly distributed among the gram-positive lineage of the eubacteria, was previously shown to exist in the extreme halophile, Halobacterium vallismortis. The results are consistent with the conclusion based upon 16S rRNA analyses that Methanomicrobiales and the extreme halophiles cluster together.
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  • 3
    ISSN: 1432-072X
    Keywords: Methyltransferase ; Membrane protein ; Corrinoids ; Methanogenic bacteria ; Archaebacteria ; Energy conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An assay is described that allows the direct measurement of the enzyme activity catalyzing the transfer of the methyl group from N 5-methyltetrahydromethanopterin (CH3−H4MPT) to coenzyme M (H−S−CoM) in methanogenic archaebacteria. With this method the topology, the partial purification, and the catalytic properties of the methyltransferase in methanol- and acetate-grown Methanosarcina barkeri and in H2/CO2-grown Methanobacterium thermoautotrophicum were studied. The enzyme activity was found to be associated almost completely with the membrane fraction and to require detergents for solubilization. The transferase activity in methanol-grown M. barkeri was studied in detail. The membrane fraction exhibited a specific activity of CH3−S−CoM formation from CH3−H4MPT (apparent K m=50 μM) and H−S−CoM (apparent K m=250 μM) of approximately 0.6 μmol·min-1·mg protein-1. For activity the presence of Ti(III) citrate (apparent K m=15 μM) and of ATP (apparent K m=30 μM) were required in catalytic amounts. Ti(III) could be substituted by reduced ferredoxin. ATP could not be substituted by AMP, CTP, GTP, S-adenosylmethionine, or by ATP analogues. The membrane fraction was methylated by CH3−H4MPT in the absence of H−S−CoM. This methylation was dependent on Ti(III) and ATP. The methylated membrane fraction catalyzed the methyltransfer from CH3−H4MPT to H−S−CoM in the absence of ATP and Ti(III). Demethylation in the presence of H−S−CoM also did not require Ti(III) or ATP. Based on these findings a mechanism for the methyltransfer reaction and for the activation of the enzyme is proposed.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 170 (1998), S. 78-84 
    ISSN: 1432-072X
    Keywords: Key wordsAspergillus nidulans ; Filamentous fungi ; Sexual development ; Cleistothecia ; Laccase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sexual development in Aspergillus nidulans is a morphogenetic differentiation process triggered by internal and environmental signals. As a first step in analyzing the developmental pathway at the molecular level, laccase II (EC 1.10.3.2), which is specifically expressed in early stages of fruitbodies, was isolated. The enzyme was purified to apparent homogeneity from a mutant strain (SMS1) in which the sexual cycle dominates and the number of cleistothecia is increased tenfold. Laccase II was enriched 560-fold to a specific activity of 892 U (mg protein)–1. The apparent molecular mass was determined to be 80 kDa under denaturing conditions and to be 100–120 kDa under native conditions. The internal peptide sequences gained from the protein will allow the isolation of the corresponding gene as a first step in determining the key regulators of sexual development.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Mineralogy and petrology 19 (1973), S. 77-80 
    ISSN: 1438-1168
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Mineralogy and petrology 22 (1975), S. 236-241 
    ISSN: 1438-1168
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Description / Table of Contents: Summary The crystal structure of schafarzikite, FeSb2O4,a 0=8.59;c 0=5.91 Å, space groupP 4mbc-D 4h 13 ,Z=4, has been refined. The atomic arrangement agrees essentially with that determined byZemann. The refined structure, using Weissenberg diffractometer data, yielded a finalR value of 0.056. The Fe atoms are surrounded octahedrally by six oxygen atoms, the Sb atoms form with three oxygen atoms a flat trigonal pyramid.
    Notes: Zusammenfassung Die Verfeinerung der Kristallstruktur von Schafarzikit, FeSb2O4,a 0=8,59;c 0=5,91 Å, RaumgruppeP 4mbc-D 4h 13 ,Z=4, bestätigte im wesentlichen die vonZemann gefundene Atomanordnung. Die Struktur konnte bei Verwendung von Weissenberg-Diffraktometerdaten aufR=0,056 verfeinert werden. Die Fe-Atome werden von sechs Sauerstoffen in Form eines Oktaeders umgeben, die Sb-Atome bilden mit drei Sauerstoffen eine flache trigonale Pyramide.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Mineralogy and petrology 11 (1966), S. 165-170 
    ISSN: 1438-1168
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Mineralogy and petrology 17 (1972), S. 196-207 
    ISSN: 1438-1168
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Description / Table of Contents: Summary The crystal structure of molybdomenite, PbSeO3, has been determined by single crystal X-ray methods and refined by the full-matrix least-squares technique. Molybdomenite crystallizes in the monoclinic space group P 21/m−C 2h 2 ,a 0=6.91,b 0=5.48,c 0=4.51, β=112° 50′;Z=2. With all abserved reflections aR=0.092. The SeO3 2−-ion has a pyramidal shape with the selenium at the vertex and three oxygen atoms at the base. Lead has the nearest four oxygen neighbours lying all to one side, but ht edistances to the next five oxygens lying to the other side are not much longer. These units are linked together to a three-dimensional structure.
    Notes: Zusammenfassung Die Kristallstruktur von Molybdomenit, PbSeO3, wurde mit Hilfe von Einkristallmethoden bestimmt und nach der Methode der kleinsten Quadrate verfeinert. Die Gitterparameter, der Elementarzelle (Raumgruppe P21/m−C 2h 2 ) betragen:a 0=6,91,b 0=5,48,c 0=4,51 Å, β=112° 50′;Z=2. Mit allen beob. Reflexen wurde einR-Wert von 0,079, unter Einschluß derHamilton-Reflexe einR-Wert von 0,092 erhalten. Die Selenatome sitzen an der Spitze einer trigonalen Pyramide, deren Basis aus drei Sauerstoffatomen besteht. Die vier nächsten Sauerstoffnachbarn des Bleis liegen zwar auf einer Seite, doch sind die weiteren fünf Sauerstoffabstände nicht viel länger. Die Baueinheiten sind zu einem dreidimensionalen Gerüst verknüpft.
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  • 9
    ISSN: 1435-1536
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 10
    ISSN: 1435-1536
    Keywords: Analyticalultracentrifuge ; fluorescencedetection ; proteins ; nucleicacids ; viruses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A fluorescence detection system was developed for the analytical ultracentrifuge Spinco model E. Fluorescence is excited by a laser beam which is focussed into the cell and illuminates an area with a dimension of 60 μm in radial direction. For scanning the laser beam is moved in radial direction. After passing the cell, the laser beam is quenched by a carbon light trap and a set of optical filters. Fluorescence emission intensity is monitored by a photomultiplier located behind the light trap and the set of filters. The sensitivity of the detection system was tested by applying it to the sedimentation analysis of proteins and nucleic acids. Bovine serum albumin (BSA) was covalently labelled with the fluorescence-dye fluorescein-isothiocyanate (FITC), and its sedimentation coefficient could be determined even if BSA was analyzed in a concentration as low as 10−10 M. Nucleic acids were labelled non-covalently by the intercalating dye ethidium bromide. Only 8 ng RNA were needed for the determination of the sedimentation coefficient. The particular advantages of the fluorescence detection system were exploited for the establishment of a new method for quantitative virus detection. To tobacco mosaic virus (TMV) a monoclonal anti-TMV antibody from mouse was bound, and to this a second, anti-mouse antibody that carried the fluorescence-label FITC was attached. Either by UV-irradiation or by incubation with glutaraldehyde, the first antibody was covalently crosslinked to TMV, and the second antibody to the first. In CsCl density centrifugation with fluorescence detection as little as 3.2 ng virus/80 μl or 6×108 virus particles/ml were recorded in a well expressed band at the corresponding buoyant density. Tenfold lower concentration would result still in a significant band. The sensitivity compares well with those of the most advanced techniques from immunology. Due to the specific labelling of viruses by antibodies it will be possible to carry out quantitative physical characterization of virus containing samples without purifying the virus. Future applications of the fluorescence detection system and of the virus detection technique are discussed.
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