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  • 1
    Digitale Medien
    Digitale Medien
    Purification and properties of an alkaline protease from alkalophilic Bacillus sp. KSM-K16 (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 473-481 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Alkaline protease (EC 3.4.21.14) activity, suitable for use in detergents, was detected in the alkaline culture medium of Bacillus sp. KSM-K16, which was originally isolated from soil. The enzyme, designated M protease, was purified to homogeneity from the culture broth by column chromatographies. The N-terminal amino acid sequence was Ala-Gln-Ser-Val-Pro-Trp-Gly-Ile-Ser-Arg-Val-Gln-Ala-Pro-Ala-Ala-His-Asn-Arg-Gly-Leu-Thr-Gly. The molecular mass of the protease was 28 kDa, and its isoelectric point was close to pH 10.6. Maximum activity toward casein was observed at 55°C and at pH 12.3 in 50 mM phosphate/NaOH buffer. The activity was inhibited by phenylmethylsulfonyl flouride and chymostatin. The enzyme was very stable in long-term incubation with liquid detergents at 40°C. The enzyme cleaved the oxidized insulin B chain initially at Leu15-Tyr16 and efficiently at ten more sites. Among various oligopeptidyl p-nitro-anilides (pNA) tested, N-succinyl-Ala-Ala-Pro-Phe-pNA was efficiently hydrolyzed by M protease. M protease was precipitated in (NH4)2SO4-saturated acetate buffer (pH 5.0) as plank-like cyrstals.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00218452
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  • 2
    Digitale Medien
    Digitale Medien
    Purification and properties of an alkaline protease from alkalophilic Bacillus sp. KSM-K16 (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 473-481 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract  Alkaline protease (EC 3.4.21.14) activity, suitable for use in detergents, was detected in the alkaline culture medium of Bacillus sp. KSM-K16, which was originally isolated from soil. The enzyme, designated M protease, was purified to homogeneity from the culture broth by column chromatographies. The N-terminal amino acid sequence was Ala–Gln–Ser–Val–Pro–Trp–Gly–Ile–Ser–Arg–Val–Gln–Ala–Pro–Ala–Ala–His–Asn–Arg–Gly–Leu–Thr–Gly. The molecular mass of the protease was 28 kDa, and its isoelectric point was close to pH 10.6. Maximum activity toward casein was observed at 55 °C and at pH 12.3 in 50 mM phosphate/NaOH buffer. The activity was inhibited by phenylmethylsulfonyl fluoride and chymostatin. The enzyme was very stable in long-term incubation with liquid detergents at 40 °C. The enzyme cleaved the oxidized insulin B chain initially at Leu15–Tyr16 and efficiently at ten more sites. Among various oligopeptidyl p-nitro-anilides (pNA) tested, N-succinyl-Ala-Ala-Pro-Phe-pNA was efficiently hydrolyzed by M protease. M protease was precipitated in (NH4)2SO4-saturated acetate buffer (pH 5.0) as plank-like crystals.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002530050437
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  • 3
    Digitale Medien
    Digitale Medien
    Low-specificity l-threonine aldolase of Pseudomonas sp. NCIMB 10558: purification, characterization and its application to β-hydroxy-α-amino acid synthesis (1998)
    Springer
    Applied microbiology and biotechnology 49 (1998), S. 702-708 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Low-specificity l-threonine aldolase, catalyzing the reversible cleavage/condensation reaction between l-threonine/l-allo-threonine and glycine plus acetaldehyde, was purified to homogeneity from Pseudomonas sp. NCIMB 10558. The enzyme has an apparent molecular mass of approximately 145 kDa and consists of four identical subunits with a molecular mass of 38 kDa. The enzyme, requiring pyridoxal- 5′-phosphate as a coenzyme, is strictly l-specific at the α position, whereas it can not distinguish between threo and erythro forms at the β position. Besides the reversible cleavage/condensation of threonine, the enzyme also catalyzes the reversible interconversion between glycine plus various aldehydes and l-β-hydroxy-α-amino acids, including l-β-(3,4-dihydroxyphenyl)serine, l-β-(3,4-met‐hylenedioxyphenyl)serine and l-β-phenylserine, providing a new route for the industrial production of these important amino acids.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002530051235
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  • 4
    Digitale Medien
    Digitale Medien
    Purification of alkaline proteases from a Bacillus strain and their possible interrelationship (1996)
    Springer
    Applied microbiology and biotechnology 45 (1996), S. 63-71 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract  Alkalophilic Bacillus sp. KSM-K16 produced three alkaline proteases, as detected by polyacrylamide gel electrophoresis (PAGE). The major protease, designated M protease, was recently purified to homogeneity and its properties were characterized. In the present study, two minor proteases, designated H protease and N protease, were purified to homogeneity from cultures of this organism. H protease had a molecular mass of 28 kDa, as estimated by sodium dodecyl sulfate/PAGE (SDS-PAGE) and its maximum activity against casein was observed at pH 11.0 and at 55°C. N protease consisted of two polypeptide chains with molecular masses of 12.5 kDa and 14.5 kDa, as estimated by SDS-PAGE, although it migrated as a single protein band during non-denaturing PAGE. Its maximum activity was observed at pH 11.0 and at 60°C. The amino-terminal sequences of H protease and of the 14.5-kDa polypeptide of N protease were identical to that of M protease. The electrophoretic relationship between the three enzymes was examined after they had been stored at different pH values and at 5°C. M protease was converted to H protease more rapidly at pH 11 than at pH 8 or below, and H protease was converted to M protease at pH 8 or below but not at pH 11. N protease appeared to be the autolytic product of the M and H proteases.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002530050650
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  • 5
    Digitale Medien
    Digitale Medien
    Catalytic decomposition of N2O over supported rhodium catalysts: high activities of Rh/USY and Rh/Al2O3 and the effect of Rh precursors (1997)
    Springer
    Catalysis letters 47 (1997), S. 173-175 
    Details
    ISSN: 1572-879X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract The catalytic decomposition of nitrous oxide to nitrogen and oxygen has been studied over Al2O3-supported and zeolite-supported Rh catalysts. The activities of Rh/Al2O3 and Rh/USY (ultrastable Y zeolite) catalysts prepared from Rh(NO3)3 were higher than those of Rh/ZSM-5 and Rh/ZnO reported in the literature, while the activity of a Rh/Al2O3 catalyst prepared from RhCl3 was suppressed severely in spite of the high H/Rh and CO/Rh values. The catalytic activity of N2O decomposition was sensitive not only to the Rh dispersion but also to the preparation variables such as the Rh precursors and the supports used.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1019017407609
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  • 6
    Digitale Medien
    Digitale Medien
    Gas contaminant effect in a discharge-excited ArF excimer laser (1998)
    Springer
    Applied physics 66 (1998), S. 579-583 
    Details
    ISSN: 1432-0649
    Schlagwort(e): PACS: 42.55
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: 4 , O2 and N2 in a discharge-excited ArF-excimer laser. Measured characteristics include laser pulse energy, small-signal gain, and laser spectrum. Measurement results indicate that laser pulse energy degradation for such impurities (〈100 ppm) is mainly due to optical absorption. It has also been found that for O2 contaminants laser pulse energy degradation is strongly dependent on operational repetition rates; at higher repetition rates, an increased concentration of O2 impurities results in a reduced small-signal gain and a consequent decline in laser pulse energy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s003400050438
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  • 7
    Digitale Medien
    Digitale Medien
    Continuous operation up to 3 kHz in a discharge-pumped XeCl excimer laser (1996)
    Springer
    Applied physics 63 (1996), S. 1-7 
    Details
    ISSN: 1432-0649
    Schlagwort(e): 42.55
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract Continuous operations at a high repetition rate up to 3 kHz for discharge-pumped XeCl excimer laser are presented. The dependence of the clearing ratio on laser-gas pressure and input energy densities is studied. It was found that suitable laser-gas pressures minimize the clearing ratio for various input-energy densities. It is also shown that few density disturbances in the discharge region are induced by gas heating in a discharge-pumped XeCl excimer laser with low input-energy density
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01112828
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  • 8
    Digitale Medien
    Digitale Medien
    Long lifetime operation of an ArF-excimer laser (1996)
    Springer
    Applied physics 63 (1996), S. 229-235 
    Details
    ISSN: 1432-0649
    Schlagwort(e): PACS: 42.55
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract.  An experimental investigation to improve the lifetime of a discharge-excited ArF-excimer laser is presented. The three dominant factors restricting its lifetime are CF4 generation in the laser gas, color-center formation in the optics and input power density reduction due to electrode ablation. Copper electrodes were superior to nickel electrodes in regard to electrode ablation. A gas lifetime of more than 109 shots (about one month at 400 Hz) is shown for an ArF-excimer laser with a liquid-nitrogen trap and high-temperature zirconium alloy trap.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01833791
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  • 9
    Digitale Medien
    Digitale Medien
    Long lifetime operation of an ArF-excimer laser (1996)
    Springer
    Applied physics 63 (1996), S. 229-235 
    Details
    ISSN: 1432-0649
    Schlagwort(e): 42.55
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract An experimental investigation to improve the lifetime of a discharge-excited ArF-excimer laser is presented. The three dominant factors restricting its lifetime are CF4 generation in the laser gas, color-center formation in the optics and input power density reduction due to electrode ablation. Copper electrodes were superior to nickel electrodes in regard to electrode ablation. A gas lifetime of more than 109 shots (about one month at 400 Hz) is shown for an ArF-excimer laser with a liquid-nitrogen trap and high-temperature zirconium alloy trap.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01833791
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  • 10
    Digitale Medien
    Digitale Medien
    Brittleness of glasses by indentation (1995)
    Springer
    Journal of materials science 14 (1995), S. 167-169 
    Details
    ISSN: 1573-4811
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318244
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