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  • 1
    Electronic Resource
    Electronic Resource
    Structure and regulation of the isocitrate lyase gene ICL1 from the yeast Saccharomyces cerevisiae (1993)
    Springer
    Current genetics 23 (1993), S. 375-381 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Isocitrate lyase ; Gene regulation ; Ethanol induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ICL1 gene encoding the isocitrate lyase from Saccharomyces cerevisiae was cloned and sequenced. A reading frame of 557 amino acids showing significant similarity to isocitrate lyases from seven other species could be identified. Construction of icl1 null mutants led to growth defects on C2 carbon sources while utilization of sugars or C3 substrates remained unaffected. Using an ICL1-lacZ fusion integrated at the ICL1 locus, a more than 200-fold induction of β-galactosidase activity was observed after growth on ethanol when compared with glucose-repressed conditions. A preliminary analysis of the ICL1 upstream region identified a 364-bp fragment necessary and sufficient for this regulatory phenotype. Sequence motifs also present in the upstream regions of co-regulated genes were found within this region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312621
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  • 2
    Electronic Resource
    Electronic Resource
    Das Problem Akmit-Ägirin (1958)
    Springer
    Contributions to mineralogy and petrology 6 (1958), S. 112-138 
    Details
    ISSN: 1432-0967
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Zusammenfassung In der vorliegenden Arbeit wurde erneut die Frage nach dem Zusammenhang zwischen der Farbe der Natriumeisenpyroxene und ihrer chemischen Konstitution aufgeworfen. Da die früheren Versuche, diese Frage auf analytischem Wege zu lösen, nicht zum Ziele geführt hatten, wurde hier das reine Endglied NaFeIIIISi2O6 synthetisch hergestellt; in weiteren Versuchen wurden Na und Fe3+ durch andere Ionen zu einem kleinen Teil ersetzt und der Einfluß auf die Farbe ermittelt Es wurden Versuche durchgeführt, bei denen Na durch Ca oder Cd, Fe3+ durch Mg, Mn2+, Fe2+, Co, Ni oder Zn ersetzt wurde; bei weiteren Versuchen wurde anstelle von Fe3+ zu einem kleinen Teil sowohl eines der obengenannten 2wertigen Ionen als auch Ti4+ oder Zr4+ eingebaut. Die erhaltenen Mischkristalle wurden optisch und röntgenographisch untersucht. Zur näheren Charakterisierung dieser Mischglieder wurde in einer Reihe von Schliffen das Absorptionsspektrum in verschiedenen kristallographischen Richtungen bestimmt und mit den ebenfalls aufgenommenen Absorptionskurven von natürlichen Ägirinen verglichen. Auf Grund der synthetischen Versuche werden folgende Definitionen vorgeschlagen: Die Namen Akmit und Ägirin sollen weiterhin für Natriumeisenpyroxen nebeneinander beibehalten werden, wobei die Abgrenzung gegenüber Ägirinaugit gemäß dem Vorschlag vonTröger (2) vorgenommen werden kann. Für die Unterscheidung der beiden Varietäten soll gemäß den bisherigen Gepflogenheiten die Farbe maßgebend sein. Das reine Endglied NaFeIIISi2O6 ist braun und muß deshalb als Akmit bezeichnet werden. Ferner sind mit Akmit die Mischkristalle zu bezeichnen, bei denen das Fe3+ durch Mn ersetzt ist, da auch diese Glieder braune Farbe aufweisen; die Ansicht vonGrout konnte also durch synthetische Versuche bestätigt werden. Die grüne Farbe der Ägirine in RichtungX undY wird durch die gleichzeitige Anwesenheit von 2- und 3wertigem Eisen hervorgerufen, so daß als Ägirin diejenigen Mischkristalle zu bezeichnen sind, bei denen 3wertiges Eisen durch zwertiges ersetzt ist. Der Einfluß des 2wertigen Eisens überwiegt dabei gegenüber dem Einfluß anderer Ionen. Das häufigere Auftreten des Ägirin gegenüber Akmit hat also seine Ursache darin, daß in der Natur 2wertiges Eisen praktisch stets vorhanden ist. Die Gitterkonstanten von Akmit und Ägirin sind im Bereich der hier erzielten Meßgenauigkeit gleich und wurden wie folgt bestimmt:a = 9,66 Åb = 879 Åc = 5,26 Åβ = 72,7 ° Die Arbeit wurde gefördert durch eine Spende keramischen Materials zum Bau der Versuchsöfen, wofür ich auch an dieser Stelle der Fa. Stemag in Lauf meinen Dank aussprechen möchte. Der Fa. Elchem in Nürnberg danke ich für die Durchführung der Spektralanalysen sowie für die Anfertigung einer Aufnahme mit der Guinier-Kammer. Ferner danke ich dem Institut für Anorganische Chemie der Universität Erlangen für Überlassung, bzw. Anfertigung einiger Substanzen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01084744
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of UAS elements and binding proteins necessary for derepression of Saccharomyces cerevisiae fructose-1,6-bisphosphatase (1992)
    Springer
    Current genetics 22 (1992), S. 363-370 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Fructose-1,6-bisphosphatase ; Glucose repression ; Gene activation ; Gluconeogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fructose-1,6-bisphosphatase is a key enzyme in gluconeogenesis and the FBP1 gene is not transcribed during growth with glucose. Genetic analysis indicated a positive regulation of FBP1 expression after exhaustion of glucose. By linker-deletion analysis, two upstream activation sites (UAS1 and UAS2) were localized and the respective UAS-binding factors (DAP I and DAP II for derepression activating protein) were identified by gel retardation. UAS1 and UAS2 span about 30 bp each, and are separated by approximately 30 bp. Both UAS sites act synergistically. Although UAS1 showed some similarities to the DNA-binding consensus for the general yeast activator Rap1, competition experiments and DEAE-chromatography proved that DAP I and Rap1 correspond to different proteins. Gel retardation by DAP I depended on carbon sources and did not occur in cells growing logarithmically with glucose, whereas a strong retardation signal was obtained with ethanol-grown cells. The present results suggest that DAP I and DAP II are the final regulatory elements for glucose derepression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00352437
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  • 4
    Electronic Resource
    Electronic Resource
    Molecular cloning and analysis of the nuclear gene MRP-L6 coding for a putative mitochondrial ribosomal protein from Saccharomyces cerevisiae (1993)
    Springer
    Current genetics 24 (1993), S. 136-140 
    Details
    ISSN: 1432-0983
    Keywords: Mitochondria ; Ribosomal protein ; Nuclear gene ; pet mutant ; yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Saccharomyces cerevisiae nuclear gene MRP-L6 was cloned by complementation of the respiratory-deficient mutant pet-ts 2523 with a library of wildtype yeast genomic DNA. The isolated gene was part of a 3.8-kb sequenced DNA fragment containing, in addition to MRP-L6, two unassigned reading frames, ORF1 and ORF2. MRP-L6 codes for a basic protein of 205 amino acids and a molecular mass of 22.8 kDa. The protein exhibits significant sequence similarity to the ribosomal protein L6 of bacteria and chloroplasts. Unlike the corresponding bacterial proteins, however, the MRP-L6 protein (MRP-L6p) contains at its N-terminus a 16 amino-acid leader sequence exhibiting the known characteristics of mitochondrial import signals. Disruption of MRP-L6 leads to the phenotype of a mitochondrial translation-defective, rho-negative yeast mutant. The results are consistent with MRP-L6p representing an essential component of yeast mitochondrial ribosomes. Expression of MRP-L6 was examined, under conditions of glucose repression and derepression, in wild-type cells and in a series of catabolite repression-defective yeast mutants. In most cases, a distinct though small influence of the carbon source on the expression of an MRP-L6/lacZ reporter construct was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00324677
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  • 5
    Electronic Resource
    Electronic Resource
    Genetical and RFLP studies at the Mla locus conferring powdery mildew resistance in barley (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 713-718 
    Details
    ISSN: 1432-2242
    Keywords: Barley ; Multigene family ; Mla locus ; Recombination ; RFLP marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The complex structure of the multigene family at the Mla locus conferring powdery mildew resistance in barley was studied by making diallel crosses between several near-isogenic lines carrying different Mla alleles. The mode of inheritance of the Mla alleles investigated was determined to be dominant for Mla1, Mla6, Mla7 and Mla13 and semidominant for Mla3, Mla12 and Mla20. F1 plants were backcrossed to the susceptible recurrent parent in order to identify susceptible and double-resistant recombinants in the BC1F1 generation. Out of 17605 progenies tested in the BC1F1 generation, two susceptible recombinants, one between Mla1 and Mla12 and one between Mla13 and Mla20 were confirmed. The former was also verified by RFLP analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225010
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  • 6
    Electronic Resource
    Electronic Resource
    RFLP markers to identify the alleles on the Mla locus conferring powdery mildew resistance in barley (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 330-338 
    Details
    ISSN: 1432-2242
    Keywords: RFLP marker ; Barley ; Powdery mildew ; Mla locus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To identify the mildew resistance locus Mla in barley with molecular markers, closely linked genomic RFLP clones were selected with the help of near-isogenic lines having the ‘Pallas’ and ‘Siri’ background. Out of 22 polymorphic clones 3 were located around the Mla locus on chromosome 5 with a distance of 5.1 + 2.9 cM (MWG 1H068), 4.2±1.7 cM (MWG 1H060) and 0.7 ± 0.7 cM (MWG 1H036), respectively. The polymorphic clone MWG 1H036 displayed the same RFLP pattern in both ‘Pallas’ and ‘Siri’ near-isogenic lines and in different varieties digested with six restriction enzymes possessing the same mildew resistance gene. The alleles of the Mla locus were grouped in 11 classes according to their specific RFLP patterns; 3 of these groups contain the majority of Mla alleles already used in barley breeding programs in Europe.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229491
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  • 7
    Electronic Resource
    Electronic Resource
    A review and integrating analysis of Spatially-Variable Control of crop production (1992)
    Springer
    Nutrient cycling in agroecosystems 33 (1992), S. 1-34 
    Details
    ISSN: 1573-0867
    Keywords: Automatic control ; fertilizer application ; geographic information systems ; sensors ; sitespecific ; spatially-variable ; variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The variability of crops, soils, and pests within crop production fields has led to attempts to understand those variations and to manage crop production accordingly. The rapidly expanding research and development on the management and control of crop production according to in-field variations is reviewed. The various stages and components of this spatially-variable control are classified and presented along with a proposed terminology and notation. Advances in component technologies (sensors, actuators, locators, geostatistics, geographic information systems) and their integration should reduce crop production costs and be environmentally advantageous.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01058007
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  • 8
    Electronic Resource
    Electronic Resource
    Über die Bestimmung der Spurenelemente in der Agrikulturchemie (1956)
    Springer
    Microchimica acta 44 (1956), S. 393-400 
    Details
    ISSN: 1436-5073
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary A method is described for determining the trace elements in soils, plants, feeds, and fertilizers. The trace elements are separated from the major constituents and concentrated by shaking with complex-forming compounds, after destroying the organic material, the concentrate obtained is dissolved in acid. These solutions are brought continuously on a disk of spectrum carbon and simultaneously spectrographed in sparks. The evaluation is made by comparison with solutions of known content in the usual manner.
    Abstract: Résumé Description d'une méthode de dosage des oligoéléments dans les sols, les plantes, la nourriture et les engrais. Les éléments sont séparés de la matière à analyser, concentrés par agitation avec des substances complexantes. Après destruction de la matière organique les concentrés obtenus sont dissouts dans un acide. Ces solutions sont amenées de façon continue sur un disque en carbone pur et analysées par spectrographie d'étincelles. La détermination se fait par comparaison avec des solutions étalons suivant la méthode classique.
    Notes: Zusammenfassung Es wird eine Methode zur Bestimmung der Spurenelemente in Böden, Pflanzen, Futter- und Düngemitteln beschrieben. Die Spurenelemente werden von den Hauptbestandteilen getrennt, durch Ausschüttelung mit komplexbildenden Verbindungen angereichert, nach Zerstörung der organischen Substanz die erhaltenen Konzentrate in Säure gelöst. Diese Lösungen werden auf einer Scheibe aus spektralreiner Kohle kontinuierlich aufgebracht und gleichzeitig im Funken spektrographiert. Die Auswertung erfolgt durch Vergleich mit Lösungen bekannten Gehaltes in üblicher Weise.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01216624
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  • 9
    Electronic Resource
    Electronic Resource
    Effects of microstructure on optimum heat treatment conditions in metal-matrix composites (1991)
    Springer
    Journal of materials science 26 (1991), S. 3287-3291 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract It is well known that the microstructure of metal-matrix composite materials is significantly different from that of the unreinforced matrix. Heat treatments which optimized strength values in the matrix therefore, no longer do so in the composite. It is thus beneficial to find these variations in the heat treatment process, and to pinpoint the microstructural mechanisms responsible. It is known that, in addition to a higher dislocation density, the composite also has a very fine grain size and many whisker interfacial nucleation sites. This study has found that the time required for solutionizing appears to be much shorter in the composite primarily due to the very small grain size. It was also observed that artificial ageing was not very effective in the composite since the precipitates nucleated and grew on the whisker interfaces, therefore, only natural ageing was necessary to achieve peak tensile and yield strengths.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01124675
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  • 10
    Electronic Resource
    Electronic Resource
    Strengthening potential of the cubic σ precipitate in Al-Cu-Mg-Si alloys (1994)
    Springer
    Journal of materials science 29 (1994), S. 239-249 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract An interesting cubic-shaped precipitate was observed in the matrix of a squeeze-cast Al-4.3 wt % Cu-2.0 wt % Mg/SiC composite which was heat-treated to a T7 condition. Although this phase had been observed by a few investigators in the past, it had never been examined in detail until now. This cubic phase generally had an edge length ranging from 30 to 50 nm and existed in volume fractions as high as 3.8%. Theoretical strengthening models predicted this phase to have good potential for precipitate strengthening. In addition, the cubic phase exhibited a low rate of coarsening at temperatures as high as 250 °C; apparently due to its low interfacial energy. Consequently, this cubic precipitate shows potential for increasing the useful temperature range of aluminium alloys and composites and could be of great importance to the aerospace and automotive industries.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00356599
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