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  • 1
    ISSN: 1432-072X
    Keywords: Protoplasts ; Fosfomycin ; Escherichia coli ; Serratia marcescens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A procedure for protoplasts formation from Escherichia coli and Serratia marcescens by treatment with fosfomycin alone is described. This method gives high and low yields of stable protoplasts from E. coli and S. marcescens respectively. In the last case numerous spheroplasts were obtained. Electron micrographs of intact cells, protoplasts and spheroplasts are shown.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary The effect of sampling conditions on the levels of adenine nucleotides, pyridine nucleotides, glycolytic intermediates and related metabolites in yeast has been studied. A systematic examination of the conditions for harvesting has shown that it can be best accomplished by rapid filtration. Delays in the handling for removal of the medium, as is usual in the process of obtaining a number of data reported in the literature, lead to important changes in some of the metabolites examined. It is also shown that when a washing is imperative it can be carried out with a methanol-water mixture (50/50, v/v) cooled at −40° without loss of intracellular concentrations of non-readily diffusible metabolites. On the basis of this experience the outline of a generally applicable procedure is presented.
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  • 3
    ISSN: 1436-5073
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Triäthylamin ist ein selektives Fällungsreagens für Phosphormolybdänsäure, das die Trennung von Orthophosphat aus Mischungen von Phosphorsäureestern und Anhydriden erlaubt. Eine einfache Methode zur Bestimmung von Orthophosphat-Triäthylamin-Molybdat wurde beschrieben. Sie beruht auf der Messung der Ultraviolett-Absorption des Komplexes. Fünf bis 250 nMol gefälltes Orthophosphat werden in 3 ml Methanol gelöst und die Absorption dieser Lösung gegen Wasser bei 320 nm gemessen. Der molare Extinktionskoeffizient des Komplexes unter diesen Bedingungen ist 2,09·107 1·Mol−1·cm−1.
    Notes: Summary Triethylamine is a selective precipitant for phosphomolybdic acid which allows separation of orthophosphate from phosphoric acid esters and anhydrides. In this paper a simple method for the estimation of the orthophosphate-triethylamine-rnolybdate complex, based on its ultraviolet absorption spectrum, is described. The precipitate, corresponding to a range of 5–250 nmole of orthophosphate, is dissolved in 3 ml of methanol and the absorbance of the solution measured at 320 nm against water. The molar absorptivity of the complex at this wavelength is 2.09 × 1071·mole−1·cm−1.
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  • 4
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Culture supernatants ofAzotobacter vinelandii andAzotobacter beijerinckii contain auxins, at least three gibberellin-like substances and three cytokinin-like substances. Treating roots of tomato seedlings with these cultures accelerates plant growth and increases yield of fruit, effects probably caused by activity of the plant hormones. Amounts of hormones produced in these cultures are similar to those produced byAzotobacter chroococcum andAzotobacter paspali.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Non-mycorrhizal plants grown 5 weeks in a low-phosphate soil with different amounts of soluble P were transplanted to soil also with different levels of phosphate and inoculated with VA mycorrhiza. The intensity of mycorrhizal infection as affected by the interaction of differents levels of phosphate in soil and in the host was examined after a further 8 weeks. In the soil with no added phosphate mycorrhizal infection was not affected by the initial P content of the plants. When 0.8 or 1.5 g K2PO4 was given per kg soil both the external and the internal P negatively influenced the infection. In some conditions a P content that was supraoptimal for infection was not for plant growth. The critical P concentration in plants depends on the age of the host. An interaction between P and N as a factor contributing to phosphate ‘toxicity’ is discussed.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 42 (1975), S. 653-660 
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The amount of nitrogen gas, oxygen, hydrogen, and methane in soil samples from a submerged rice field was determined using the modified Koyama's apparatus and gas chromatography. Soils planted to rice had significantly larger amounts of nitrogen gas than unplanted soils. The amount of N2 in a field soil increased after transplanting and reached a maximum at tillering. The amount of N2 in unplanted submerged soils did not increase significantly during the same period.
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  • 7
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Culture supernatants ofAzotobacter vinelandii andAzotobacter beijerinckii contain auxins, at least three gibberellin-like substances and three cytokinin-like substances. Treating roots of tomato seedlings with these cultures accelerates plant growth and increases yield of fruit, effects probably caused by activity of the plant hormones. Amounts of hormones produced in these cultures are similar to those produced byAzotobacter chroococcum andAzotobacter paspali.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Transition metal chemistry 3 (1978), S. 147-152 
    ISSN: 1572-901X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The association of H+ and Cu2+ with malonate, maleate, succinate and phthalate ions has been investigated both potentiometrically and calorimetrically at 25° and I = 0.1 mol dm−3 (NaClO4). The structures of the complexes existing in aqueous solution are proposed on the basis of thermodynamic data.
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  • 9
    ISSN: 1572-901X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The thermodynamic quantities relative to the protonation and the complexation of 2,2′,2′'-terpyridine with copper(II) ion have been determined at 25° and I=0.1 mol dm−3 (NaNO3). The ΔG∘ data was obtained by potentiometric measurements; a copper selective electrode was employed for the study of the complexation equilibria. The ΔH∘ values have been determined by direct calorimetry. The hydrolytic species, existing at pH〉6 and their relative ΔG∘ and ΔH∘ values have also been obtained. From the thermodynamic data the importance of enthalpy and entropy terms in stabilizing the complexes is assessed. Moreover some considerations on the chelating effect are reported.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Molecular and cellular biochemistry 17 (1977), S. 147-149 
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary The lower Vmax of 6PGDH with respect to G6PDH and its higher sensitivity to inhibition by NADPH, suggest the existence of an imbalance between the two dehydrogenases of the pentose phosphate pathway in rat liver. Possible modulators of these activities, particularly in relation with the inhibition by NADPH in physiological conditions, have been investigated. The results suggest that in both cases the inhibition by NADPH is strictly isosteric and that the relative affinities for the reduced and oxidized forms of the pyridine nucleotide are unaffected by glutathion, the intermediates of the pentose phosphate shunt or some divalent ions.
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