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  • 1
    ISSN: 1432-0983
    Keywords: Transportable elements ; Insertion mutations ; Expression mutants ; Yeast CYC7 gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The plasmid YCpCYC7(2) was constructed containing the Saccharomyces cerevisiae CYC7 gene, encoding the iso-2-cytochrome c protein, replicative sequences and selective markers from both E. coli and yeast, and the centromere of yeast chromosome III. The expression of the plasmid-CYC7 gene in yeast was similar to the low level expression characteristic of the chromosomal CYC7 gene. A number of insertions into the sequences 5′ to the gene were constructed in vitro. The insertion at 142 by 5′ to the coding sequence of a 400 by fragment which lies 5′ to the CYC1 gene and is known to be essential for the high rates of CYC1 transcription increased transcription of the CYC7 gene to levels characteristic of CYC1 transcription. On the other hand, the insertion of random DNA fragments at the same position gave mostly decreased CYC7 transcription. In addition to these in vitro constructions, a mutant plasmid was selected which had increased CYC7 transcription. This mutation was caused by the insertion of the bacterial IS1 element 313 by 5′ to the CYC7 coding sequence. The significance of these results is discussed in terms of two alternative models for CYC7 gene expression.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Space science reviews 27 (1980), S. 555-561 
    ISSN: 1572-9672
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary Using values of d, γmin, and γmax that Van Riper (1978) has found most promising for a hydrodynamic envelope ejection, we have shown that even a small amount of rotation in the initial core can stop its collapse before nuclear densities are reached. We expected χi 〉 0.02 to produce significant deviations from a spherically symmetric collapse, but have found that χi as much as ten times smaller than this will not allow the core to reach densities as high as in the spherical collapse. In no case, however, does the core flatten very much, nor does the value of β become very large. Low final β's preclude the formation of an axisymmetric torus. They also indicate that deformation of an iron core into a triaxial configuration or fragmentation of the core during its collapse is an extremely unlikely event. (Note: Classically, β must exceed 0.27 before a dynamic instability to non-axisymmetric perturbations is encountered.) The small degree of flattening of the core also suggests that the reduced moment of inertia I of the core will always be relatively small in magnitude and hence that the third time derivative of I, which is proportional to the energy emitted in gravity wave radiation, will not be very significant. Numerically calculated estimates of I- during some of these model evolutions supports this suspicion. If the γmin and used here are found to be realistic values after the detailed physics of the core collapse is well understood, it is clear that gravitational radiation from a core collapse will be difficult to measure. Finally, we should point out that it is the relatively large values of Ymin (near 4/3) combined with values of d near unity that (a) prevented the core from flattening significantly in these models and (b) prevented the core from reaching high β configurations. If “realistic” values of either one (or both) of these parameters are found to be much smaller in more complete models of the core collapse, then the core will have to become flatter (and denser) before pressure gradients will support it along the rotation axis. All of the conclusions drawn here would be modified accordingly under those circumstances. It should also be noted that in general relativistic models, the critical Γ for spherical collapse is somewhat larger than 4/3 (Van Riper, 1979). Therefore, we predict that when fully general relativistic core collapses are performed including rotation, a given choice of γmin and βi will produce a slightly flatter and slightly denser core than the corresponding model that has been presented here.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Space science reviews 27 (1980), S. 255-259 
    ISSN: 1572-9672
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Numerical calculations of the collapse of adiabatic clouds from uniform density and rotation initial conditions show that when restricted to axisymmetry, the clouds form either near-equilibrium spheroids or rings. Rings form in the collapse of low thermal energy clouds and have β = T/ ¦W¦≳ 0.43. When the axisymmetric constraint is removed and an initial m=2 density variation is introduced, clouds either collapse to form near-equilibrium ellipsoids or else fragment into binary systems through a bar phase. Ellipsoids form in the collapse of high thermal energy clouds and have 3 ≲ 0.27. The results are consistent with the critical values of β for instabilities in Maclaurin spheroids, and suggest that protostellar clouds may undergo a dynamic fragmentation in the nonisothermal collapse regime.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 120 (1984), S. 209-215 
    ISSN: 1615-6102
    Keywords: Fusion ; Calcium ; Protoplast ; Membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Rather than selecting for a chemical fusogen one can select for a fusogenic plant membrane (i.e., one that will fuse readily). Wild carrot suspension culture cells can be grown under conditions which cause the released protoplasts to have a high potential to fuse. Protoplast fusion is enhanced by calcium and inhibited by EGTA. When 10mM calcium (pH6.0) is added, fusion percentages of 60% are common. The mild fusion treatment appears to have no effect on callus regeneration and differentiation.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 123 (1984), S. 213-220 
    ISSN: 1615-6102
    Keywords: Carrot suspension cultures ; Dictyosomes ; Endoplasmic reticulum ; Golgi apparatus ; Membrane biogenesis ; Plasma membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Wild carrot (Daucus carota L.) cells, grown in suspension culture, were labeled with radioactive precursors and fractionated into constituent membranes to be analyzed for specific radioactivity. Results show rapid incorporation of [3H] leucine into endoplasmic reticulum (ER)-, Golgi apparatus-, and plasma membrane/tonoplast-enriched fractions. The time lag between incorporation into ER and its appearance in Golgi apparatus or plasma membrane/tonoplast were less than 5 minutes. With an average time of 3–4 minutes for cisternal formation estimated from studies with monensin, and an average of 5 cisternae per dictyosome (total transit time of 15–20 minutes), it was not possible to account for early incorporation of radioactivity into plasma membranes by passage of proteins from ER to plasma membrane via the Golgi apparatus. To account for the findings, it would appear that at least some proteins were delivered to the plasma membrane via the first membranes that exited (i.e., mature face vesicles) from the Golgi apparatus post-pulse and that some of these proteins had been translated and inserted into membranes at or near the mature face of the Golgi apparatus.
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  • 6
    ISSN: 1615-6102
    Keywords: Golgi apparatus ; Endoplasmic reticulum ; Polyribosomes ; Golgi apparatus-associated polyribosomes ; Wild carrot (Daucus carota)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Endoplasmic reticulum-polyribosome-Golgi apparatus associations were a general feature of cells of suspension cultures of wild carrot (Daucus carota L.). Free polyribosomes occurred within the Golgi apparatus zone for all dictyosomes and with equal frequency at all levels within the stack including the most mature or trans face. When evaluated and quantified from electron micrographs, approximately 60% of the dictyosome profiles were characterized by a system of transition elements consisting of part smooth-part rough endoplasmic reticulum. These were encountered most frequently in the immediate vicinity of the immature, forming or cis face, usually toward the periphery of the stacked cisternae. Analysis of serial sections showed that those dictyosome profiles not exhibiting this characteristic did so primarily because of an unfavorable plane of sectioning. All dictyosomes examined in 5 or more serial sections revealed some type of close association with endoplasmic reticulum. Some of the associations were so close that direct connections between Golgi apparatus and endoplasmic reticulum tubules could not be excluded. Also present, especially at the forming or cis face, were small 600 nm transition vesicles with nap-like surface coats on nearly 90% of the dictyosomes examined. More than 50% exhibited spiny (clathrin-)coated vesicles at the mature or trans face.
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  • 7
    Publication Date: 1983-04-01
    Print ISSN: 0172-8083
    Electronic ISSN: 1432-0983
    Topics: Biology
    Published by Springer
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  • 8
    Publication Date: 1980-01-01
    Print ISSN: 0038-6308
    Electronic ISSN: 1572-9672
    Topics: Physics
    Published by Springer
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  • 9
    Publication Date: 1980-01-01
    Print ISSN: 0038-6308
    Electronic ISSN: 1572-9672
    Topics: Physics
    Published by Springer
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  • 10
    Publication Date: 1982-12-13
    Print ISSN: 0014-5793
    Electronic ISSN: 1873-3468
    Topics: Biology , Chemistry and Pharmacology
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