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Expression of glycoconjugates on normally developing and immunologically impairedHymenolepis diminuta (1988)

Schmidt, Jürgen

Springer

Parasitology research 75 (1988), S. 155-161

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ISSN: 1432-1955

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract The carbohydrates on the surface ofHymenolepis diminuta were analyzed with gold-labelled lectins, and it was found that the surface coat of the anterior body differs from that of the strobila in its lectin-binding properties. Binding sites for lectins fromAbrus precatorius (APA),Arachis hypogaea (PNA),Glycine max (SBA) and for wheat germ agglutinin (WGA) and succinylated WGA were located on the scolex and strobilation zone. Lectin-gold particles attached mainly to the electron-dense spines. The surface coat may therefore expose sugar residues of theN-acetylglucosamine and galactose types. In contrast, the strobila had few binding sites for the above-mentioned lectins but bound concanavalin A (ConA). Lectins fromDolichos biflorus (DBA) andUlex europaeus (UEA-I) were not bound toH. diminuta. In juvenile worms from rats, the extension of the WGA- and SBA-positive region of the strobilation zone increased in length with the development of the worms. Lectin binding in juveniles from mice was similar when the mice had been immunosuppressed with cortisone. After the onset of the immune defense againstH. diminuta in nontreated mice, a moderate expression of lectin-binding substance also occurred on the strobila. Destrobilated worms were entirely covered with theN-acetylglucosamine- and galactose-containing glycoconjugates, and it is suggested that these worm remnants correspond to the lectin-binding part of normal, growing juveniles. The presence of the carbohydrates is discussed with respect to the relative resistance of the scolex-strobilation zone ofH. diminuta to immune rejection.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00932716

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Localization of glycoconjugates at the tegument of the tapeworms Hymenolepis nana and H. microstoma with gold labelled lectins (1987)

Schmidt, Jürgen ; Peters, Werner

Springer

Parasitology research 73 (1987), S. 80-86

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ISSN: 1432-1955

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract Gold labelled lectins were used for electron microscopic localization of carbohydrate components of the tegument surface of two tapeworm species, Hymenolepis nana and H. microstoma. WGA, succinylated WGA, SBA, APA, PNA and, to a lesser extent, Con A were preferentially bound to the spines of the microtrichs. UEA-I and DBA were not adsorbed. The results indicate that the surface coat of both species has exposed N-acetylglucosamine, galactose and perhaps glucose and/or mannose residues. The location of lectin-binding glycoconjugates within the tegument and parenchyma was found using the light microscope on sections of material embedded in Lowikryl K4M after lectin-gold labelling and silver enhancement of the gold grains. The tegument selectively adsorbs WGA and SBA and strongly; adsorbtion of PNA and Con A is less intense. Strong adsorbtion of DBA and PNA was confined to the basal lamina. The parenchyma adsorbed Con A, PNA and DBA, but little WGA and SBA. The results indicate that many glycoconjugates are present in the tegument. They have similar terminal sugar residues to those of the surface coat. The significance of these carbohydrates for hostparasite interactions is discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00536340

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