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  • Springer  (210)
  • 1985-1989  (207)
  • 1920-1924
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  • 1
    ISSN: 1440-1703
    Keywords: Abies ; Advanced regeneration ; Competition ; Forest development ; Tree size distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The progress of growth of a subalpine youngAbies veitchii andA. mariesii forest during 25 years was analyzed on the basis of measurements of the processes of height growth of about 230 trees in a quadrat where the old canopy had been completely destroyed by a typhoon in 1959. The original forest floor sapling population had consisted of trees shorter than 2 m. Saplings grew faster after the breakage of the canopy than before,A. veitchii growing faster thanA. mariesii. During the 25 years of growth, a few well developed trees exceeded 6 m in height, while others remained around only 1 m or less. Some small trees, mostlyA. veitchii died at sites of high density. A bimodality in the distribution of tree height had developed with a trough at about 2.5 m, differentiating the trees into canopy and suppressed populations. Canopy trees grew with wide variation of rates, while most of the suppressed trees showed little recent growth. No difference was found in recent growth rates between the two canopyAbies species. Differences in height growth rates among individual canopy trees were analyzed on the basis of their horizontal crown overlapping. Competition models evaluating the difference in height between trees with overlapping crowns were shown to be effective. The height growth rate of a canopy tree appeared to be controlled by both the closely grown taller trees and the local density of trees including those shorter than the subject tree.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary In order to produce tuna (Thunnus thynnus) growth hormone (GH), expression plasmid (pUES13S) carrying tuna GH cDNA was constructed using a vector (pKK223-3), in which the replication origin was replaced with that of pUC19. The expression of the tuna GH cDNA was greatly affected by the distance between a Shine-Dalgarno (SD) sequence and the initiation codon (ATG) and was most efficient when the distance was adjusted to 13 base pairs (bp). The amount of tuna GH produced by Escherichia coli JM109 with pUES13S was more than 12.5% of the total cytosolic proteins and the product was immunologically identified to be tuna GH (mol. wt. 21 000) by Western blot analysis using tuna GH specific immunoglobulin G (IgG). Another plasmid (pUES13S-2) containing tandemly polymerized tuna GH cDNA was constructed, to improve the productivity of tuna GH. When E. coli JM109 carrying pUES13S-2 was incubated at 40°C, the amount of tuna GH produced reached about 20% of the total cytosolic proteins.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 1986-12-01
    Print ISSN: 0912-3814
    Electronic ISSN: 1440-1703
    Topics: Biology
    Published by Springer
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  • 4
    Publication Date: 1989-02-01
    Print ISSN: 0175-7598
    Electronic ISSN: 1432-0614
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Published by Springer
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 44 (1988), S. 338-340 
    ISSN: 1420-9071
    Keywords: Congenital hydrocephalus ; subcommissural organ ; cerebral aqueduct ; CWS/Idr rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The subcommissural organ (SCO) of the congenital hydrocephalus spontaneously occurring in CWS/Idr rats was severely reduced in size and displaced at some distance from the anterior end of the cerebral aqueduct. The cerebral aqueduct of the hydrocephalic rats was open throughout its total length during postnatal days 1–20, though it was somewhat narrower at its middle region than in the normal brain.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Mathematische Annalen 276 (1987), S. 487-497 
    ISSN: 1432-1807
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Mathematische Zeitschrift 202 (1989), S. 299-311 
    ISSN: 1432-1823
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 85 (1985), S. 225-231 
    ISSN: 1432-1424
    Keywords: dielectric constant ; fluorescence study ; liposomes ; calcium effect ; phase transition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Local dielectric constant was evaluated from the Stokes shifts of fluorescence spectra ofl-α-dansylphosphatidylethanolamine (DPE) incorporated into liposomes made of synthetic phosphatidylcholine (dipalmitoyl or distearoyl) or bovine brain phosphatidylserine. The evaluation was established as follows. First, the Stokes shift of DPE was assured to follow Mataga-Lippert's equation and was a function of the dielectric constant and the refractive index in some standard organic solvents. Second, the change of the refractive index did not contribute much to the change in the Stokes shift. Third, the time resolved fluorescence depolarization of DPE in liposomes showed that the cone wobbling diffusion was rapid relative to the fluorescence lifetime and therefore that the dielectric relaxation did not affect the evaluation of the constant in the polar region of membranes. We then investigated the characteristics of the local dielectric constant in the polar region of the lipid bilayer and found that the dielectric constant varies between 4 and 34 depending upon calcium binding and also gel/liquid-crystal phase transition. Such large changes of the local dielectric constant were further correlated with the dynamic structure of lipid bilayer membranes measured by conventional fluorescence depolarization techniques. The large changes of dielectric constant around the polar region suggest that electrostatic interactions at this region can be altered 10-fold by such ionic or thermotropic factors and therefore that local dielectric properties can play crucial roles in membrane functions.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 96 (1987), S. 187-191 
    ISSN: 1432-1424
    Keywords: phospholipase C ; liposomes ; fluorescence study ; local dielectric property ; rotational freedom
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Activity of phospholipase C fromClostridium perfringens on liposomes made fromsn-3-phosphatidylcholine, dimyristoyl (DMPC), dipalmitoyl (DPPC) or distearoyl (DSPC) was measured at various temperatures and was correlated with their gel/liquid-crystalline phase transitions (T c : 23, 41.5, 52°C for DMPC, DPPC, DSPC, respectively). In all cases, the activity of phospholipase C was high in the gel phases of the substrates and was almost zero in their liquid-crystalline phases. Fluorescence depolarization measurements of N-dansyl-sn-3-phosphatidylethanolamine (DPE) and 1,6-diphenyl-1,3,5-hexatriene (DPH) incorporated into the liposomes showed that both the head group and the alkyl chains of the lipids were immobilized in the gel phases but were highly mobile in the liquid-crystalline phase. These results indicate that the rotational mobility of lipids (both of the head groups and the alkyl chains) was not a major factor in the phospholipase C reaction. It is inferred that some electrostatic and/or hydrophobic interactions might play important roles in regulation of the phospholipase C activity.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular evolution 26 (1987), S. 24-33 
    ISSN: 1432-1432
    Keywords: Protein evolution ; Population genetics ; Mutation rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary From the standpoint of the neutral theory of molecular evolution, it is expected that a universally valid and exact molecular evolutionary clock would exist if, for a given molecule, the mutation rate for neutral allelesper year were exactly equal among all organisms at all times. Any deviation from the equality of neutral mutation rate per year makes the molecular clock less exact. Such deviation may be due to two causes: one is the change of the mutation rate per year (such as due to change of generation span), and the other is the alteration of the selective constraint of each molecule (due to change of internal molecular environment). A statistical method was developed to investigate the equality of evolutionary rates among lineages. This was used to analyze protein data to demonstrate that these two causes are actually at work in molecular evolution. It was emphasized that departures from exact clockwise progression of molecular evolution by no means invalidates the neutral theory. It was pointed out that experimental studies should be done to settle the issue of whether the mutation rate for nucleotide change is more constant per year or per generation among organisms whose generation spans are very different.
    Type of Medium: Electronic Resource
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