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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Planta 166 (1985), S. 439-445 
    ISSN: 1432-2048
    Schlagwort(e): Cell culture (nucleotides) ; Datura ; Energy charge ; Nucleoside diphosphate sugar ; Nucleotide pool
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The concentrations of 17 nucleotides and three nucleosides have been determined in a batch suspension culture of Datura innoxia using a new procedure for extraction, purification and high-performance liquid chromatography separation of these compounds. The nucleotide pools change appreciably in the different phases of growth. These changes indicate the preparation for and initiation of cell proliferation, and reflect metabolic events during cell division, cell elongation and starvation. The main components of the nucleotide pool are uracil nucleotides, with uridine 5′-diphosphate sugars as the predominant fraction, and the adenine nucleotides. Although their concentrations vary by a factor of more than 6 the ratio of the uracil to adenine nucleotides is kept fairly constant during growth. The energy charge is maintained at a rather high value. The correlation of these events with nutrient uptake and macromolecular synthesis by the batch culture is presented in the following paper.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Planta 166 (1985), S. 446-451 
    ISSN: 1432-2048
    Schlagwort(e): Datura ; Macromolecular synthesis ; Nucleotide pool ; Nutrient uptake ; Suspension culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A general picture of the metabolic events which govern to growth behaviour of a batch culture of suspended dedifferentiated cells of Datura innoxia is obtained by following both the uptake and accumulation of the medium phosphate and sucrose by the cells, and the synthesis of RNA, protein and starch. The results are compared with the changes in the nucleotide pools described in the preceding paper. The sequence of formation and the regulatory dependencies of cellular pools of phosphate, sucrose, nucleotides and RNA in the production of proteins, starch and cell mass, and in the control of proliferation and cell growth are discussed. Furthermore, the importance of the maintenance pools for metabolic survival during starvation is emphasized.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Applied physics 39 (1986), S. 257-268 
    ISSN: 1432-0630
    Schlagwort(e): 42.10 ; 42.20 ; 78.20
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau , Physik
    Notizen: Abstract Measurements of the reflectance and transmittance in the infrared part of the spectrum (400...4000cm−1) are carried out on oxidized Si wafers. Intrinsic dielectric properties of the oxide layers prepared by various methods are derived from experimental data. The dielectric functions of the oxides can be fitted by oscillator models. The related model parameters are compared with those of other SiO2 samples, as crystals and glasses. Optical arrangements to detect and characterize layers of thicknesses down to 3 nm are discussed. In particular, it is shown that experiments with polarized light at oblique incidence up to 80° are a powerful tool to characterize those layers. Experiments are reported to determine, in addition, extrinsic properties such as B and P atoms in the oxide layer or defects due to an ion implantation treatment. Conditions are discussed which should be applied to obtain the best quantitative analysis of the defect concentration.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    ISSN: 1434-601X
    Schlagwort(e): 21.60.Cs ; 23.2.0.-g ; 27.80.+w
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract Gamma rays and conversion electrons have been measured in211Bi populated by the209Bi(t,p) reaction, and the near yrast levels were observed up to 21/2−. The 21/2− state is isomeric withT 1/2=70(5) ns. No alpha decaying isomer was found in211Bi. ObservedM1 transitions reveal mixing of the210Pb parent states in the levels of211Bi. The levels and transitions are well reproduced by the shell model with experimental matrix elements and pure configurations.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Annals of operations research 14 (1988), S. 125-145 
    ISSN: 1572-9338
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik , Wirtschaftswissenschaften
    Notizen: Abstract This paper presents an application of parallel computing techniques to the solution of an important class of planning problems known as generalized networks. Three parallel primal simplex variants for solving generalized network problems are presented. Data structures used in a sequential generalized network code are briefly discussed and their extension to a parallel implementation of one of the primal simplex variants is given. Computational testing of the sequential and parallel codes, both written in Fortran, was done on the CRYSTAL multicomputer at the University of Wisconsin, and the computational results are presented. Maximum efficiency occurred for multiperiod generalized network problems where a speedup approximately linear in the number of processors was achieved.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    ISSN: 1573-0867
    Schlagwort(e): Durum ; grain yield ; irrigation ; modoc ; nitrogen ; response surface model ; seeding rate ; Triticum turgidum ; yield components
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Interactive effects of nitrogen (N) rates, seeding (S) rates and irrigation frequencies on grain yield and yield components of durum wheat were studied for four years under field conditions at Tulelake, California. Each year the experiment was conducted using a split-plot design with 4 irrigation frequencies as main plots and combinations of 5 N-rates (0 to 360 kg/ha) and 5 S-rates (50 to 250 kg/ha) as subplot treatments replicated 4 times. A quadratic response surface model (RSM) was used to study the effects of these treatments on grain yield and yield components (tillers/area, kernel number/spike, kernel weight/spike and 100-seed weight). The RSM was very effective for analysis and data reduction for estimating the optimum combinations of N and S for maximizing the grain yield and yield components. The N utilization and uptake efficiency increased with each irrigation treatment and peaked at irrigation treatment C. Both N and uptake utilization efficiency decreased with each increment of N-rate. In most cases, the effect of irrigation was independent of N and S. One irrigation at tillering increased grain yield and yield components significantly over only a preplant irrigation. The response of additional irrigations were comparatively small and significant only in some cases. Both N and S had significant effects on grain yield and yield components, however, the response of N was larger than that of S. With increasing N-rate, grain yield and tiller number increased with the expected peak beyond 360kg N ha−1 but the increments beyond 180 kg N ha−1 were of progressively smaller magnitude. The kernel number and kernel weight per spike also increased with N-rate giving a peak between 270 and 360 kg N ha−1. With increasing S grain yield and tiller number/area increased while kernel number and kernel weight per spike decreased progressively. It was impossible to maximize yield and yield components at a given combination of N, S, and irrigation. According to the model, grain yield and tiller number were maximized at the highest level of N and S, while kernel number and kernel weight/spike were maximized at the lowest S (50 kg ha−1) and about 314 kg N ha−1 under adequate water supply. On the basis of the findings of this study and output of the model, 180–360 kg N ha−1, 150–250 kg S ha−1 and two post-sowing irrigations (at tillering and at boot stage) in addition to a preplant irrigation was recommended for optimum yield. An additional irrigation might be required depending on the weather conditions during the grain filling period.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Hyperfine interactions 22 (1985), S. 385-403 
    ISSN: 1572-9540
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract Emerging evidence for deformed structures in medium mass nuclei is reviewed. Included in this review are both nuclei that are ground state symmetric rotors and vibrational nuclei where there are deformed structures at excited energies (shape coexistence). For the first time. Nilsson configurations in odd-odd nuclei within the region of deformation are identified. Shape coexistence in nuclei that abut the medium mass region of deformation is also examined. Recent establishment of a four-particle, four-hole intruder band in the doublesubshell closure nucleus96Zr56 is presented and its relation to the Nuclear Vibron Model is discussed. Special attention is given to the N=59 nuclei where new data have led to the reanalysis of97Sr and99Zr and the presence of the [404 9/2] hole intruder state as isomers in these nuclei. The low energy levels of the N=59 nuclei from Z=38 to 50 are compared with recent quadrupole-phonon model calculations that can describe their transition from near-rotational to single closed shell nuclei. The odd-odd N=59 nuclei are discussed in the context of coexisting shape isomers based on the (p[303 5/2]n[404 9/2])2− configuration. Ongoing in-beam (t.p conversion-electron) multiparameter measurements that have led to the determination of monopole matrix elements for even-even42Mo nuclei are presented, and these are compared with initial estimates using lBA-2 calculations that allow mixing of normal and cross subshell excitations. Lastly, evidence for the neutron-proton3S1 force's influence on the level structure of these nuclei is discussed within the context of recent quadrupole-phonon model calculations.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 123 (1942), S. 71-77 
    ISSN: 1618-2650
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    ISSN: 1615-6102
    Schlagwort(e): Electrogenic proton pump ; Living cell fragments ; Membrane potential ; Membrane surface coat ; Physarum polycephalum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Small, spherical cell fragments derived from macroplasmodia of the acellular slime moldPhysarum polycephalum by incubation in a 15 mM caffeine solution were investigated with morphological and electrophysiological techniques. Analysis of cell surface composition with the fluorescence microscope and different RITC-conjugated lectins revealed strong binding of ConA and RCA-I, weak binding of PEA, DBA and WGA and no binding of UEA-I. In addition, binding sites for external calcium ions were detected by chlorotetracycline-fluorescence. Electron microscopical staining with ruthenium red, iron or lanthanum delivered evidence for localization of lectin and calcium binding sites in a thin mucous layer on the cell surface. Electrical recordings by means of intracellular microelectrodes yielded an average membrane potential (MP) of −113 mV. Spontaneous depolarizations of the MP, with amplitudes between 10 and 80 mV and a duration of 20–30 s, failed to show a correlation with contractile activity. The ionic nature of MP was studied by varying the composition of the perfusing medium. The MP was not much affected by changes in external [Ca2+], [K+], or [Na+] but was sensitive to changes in [Cl−] or [H+], with a linear dependence on pH0 in the range between 7 and 5. Metabolic inhibition by potassium cyanide or low temperature (11°C) as well as application of the protonophore CCCP caused a depolarization of the MP. The results strongly support the hypothesis that the MP inPhysarum cell fragments is mainly generated by an electrogenic H+-extrusion mechanism.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Springer
    Journal of comparative physiology 156 (1986), S. 563-571 
    ISSN: 1432-136X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Ketone body concentration, 3-hydroxybutyrate oxidation rates in vivo and in vitro in the hepatopancreas and the activities of enzymes of ketone body metabolism in mitochondrial samples from different tissues were investigated in normal specimens ofBiomphalaria glabrata and under conditions of starvation and of infection withSchistosoma mansoni. 1. Total ketone body concentration in the hemolymph is about 700 μM in normal snails and about 80 μM in snails starved for 5 days. In the hemolymph of infected snails the concentration of total ketone bodies is at a decreased level of 400–450 μM between days 12 and 31 p.i. Significantly increased concentrations of total ketone bodies are found on days 38 and 45 p.i. with about 1300 and 950 μM, respectively. 2. Oxidation rates of 3-hydroxybutyrate decrease significantly under conditions of starvation and infection compared with the normal state. 3. All enzymes of the HMG-CoA cycle as well as 3-oxoacid-CoA transferase are present in the hepatopancreas ofBiomphalaria glabrata. A complete HMG-CoA cycle can only be found in the hepatopancreas, other tissues lacking HMG-CoA synthase. 4. In general the activities of ketone body metabolism decrease under the conditions of starvation and infection withSchistosoma mansoni. Only in the ovotestis and head-foot-region of snails infected for 31 days do the activities of acetacetyl-CoA thiolase and 3-oxoacid-CoA transferase increase as compared with the normal state. 5. It is concluded that decreased ketone body concentration in the hemolymph of starved snails is due to decreased ketogenesis as a result of lacking substrate in this metabolic pathway. Increased concentrations of ketone bodies in snails infected for 38 days are mainly caused by decreased ketolytic activities in the tissues.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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