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  • 1
    Electronic Resource
    Electronic Resource
    A Methyl Cytidine from the Ribonucleic Acid of Anacystis nidulans (1960)
    [s.l.] : Nature Publishing Group
    Nature 186 (1960), S. 238-239 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Freshly harvested cells grown in a continuous culture chamber1 were used for isolating ribonucleic acid from Anacystis nidulans. It was found that the method of Ogur and Rosen2 was most suitable. Ribonucleic acid constitutes 0 -37-0 -50 per cent of the fresh weight of the cells. Ribonucleic acid ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/186238a0
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  • 2
    Electronic Resource
    Electronic Resource
    The age of (a tiny part of) the Australian continent (1985)
    [s.l.] : Nature Publishing Group
    Nature 317 (1985), S. 559-560 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SCH„RER and All¨gre1, using isotope dilution analysis of single grains and fragments of zircon, recently failed to substantiate a report by Froude et al.2, based on the ion microprobe SHRIMP3, of zircons older than 4,100 Myr from Mt Narryer, Western Australia. In their discussion, Sch¤rer and ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/317559c0
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  • 3
    Electronic Resource
    Electronic Resource
    Perception of apparent motion by commissurotomy patients (1986)
    [s.l.] : Nature Publishing Group
    Nature 320 (1986), S. 358-359 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We investigated motion perception by commissurotomy patients whose corpus callosum, anterior commissure, hippo-campal commissure and massa intermedia (when encountered) had been sectioned surgically for the treatment of intractable epilepsy3'4. An intact corpus callosum is presumed to be involved ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/320358a0
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  • 4
    Electronic Resource
    Electronic Resource
    Bypassing prefertilization barriers to hybridization in Nicotiana using in vitro pollination and fertilization (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 665-671 
    Details
    ISSN: 1432-2242
    Keywords: Wide hybridization ; Tissue culture ; Ovary culture ; Tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In vitro pollination of placenta attached ovules was useful in bypassing unilateral incongruity barriers for several Nicotiana interspecific hybrid combinations (N. tabacum cv. ‘Ky 17’ X N. amplexicaulis, ‘Ky 17’ X N. benthamiana, and ‘Ky 17’ X N. repanda). By measuring the pollen tube growth over time, prefertilization barriers were determined to be the cause of the incongruity. Seedling necrosis was a problem in the development of the N. amplexicaulis hybrid and it prevented maturation of the N. repanda hybrid. Callus produced from cotyledons of the N. amplexicaulis hybrid eventually resulted in plants that survived to maturity. This procedure was not successful for the N. repanda materials. The N. amplexicaulis and N. benthamiana hybrids were sterile but following chromosome doubling by midrib culture, male and female fertile plants were produced. Conventional hybridization, fertilized ovule culture, and in vitro pollination were unsuccessful in obtaining hybrids of ‘Ky 17’ crossed with N. arentsii or N. bonariensis. Apparently, strong postfertilization barriers prevent the production of viable seed of these hybrids. Each of the N. repanda — N. tabacum reciprocal hybrids could not be rescued using callus culture; this adds support to the existence of strong sexual postfertilization barriers. A recent report, however, showed that it was possible to obtain this hybrid using the technique of somatic hybridization. Thus, it appears that it may also be possible to obtain asexual hybrids of N. arentsii and N. bonariensis with N. tabacum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260773
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  • 5
    Electronic Resource
    Electronic Resource
    In vitro regeneration in Trifolium. 1. Direct somatic embryogenesis in T. rubens (L.) (1988)
    Springer
    Plant cell, tissue and organ culture 15 (1988), S. 33-45 
    Details
    ISSN: 1573-5044
    Keywords: somatic embryogenesis ; tissue culture ; histology ; Trifolium ; zygotic embryogenesis ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The origin and development of zygotic and somatic embryos of Trifolium rubens L. was studied with the aid of paraffin sections and light microscopy. Zygotic embryos were collected, fixed and prepared daily from one to ten days after cross-pollination. Somatic embryos were obtained by plating petiole sections on modified L2 medium with 0.015 mgl-1 picloram and 0.1 mgl-1 6-BAP. Cultured petioles were collected and fixed daily from one to 25 days after plating. Two regions in the vascular bundle sheath of cultured petioles gave rise to callus. The first region was adjacent to the phloem fibers and produced friable callus. The second region gave rise to compact callus that was connected to the fascicular cambium. Somatic embryos originated from single cells in the cortex directly without intervening callus formation and from single cells in the friable callus. In addition, embryos arose from meristematic regions in compact callus. Many early stages of embryogenesis (one, two and four-celled stages) were observed in the cortex and friable callus. Zygotic embryogenesis in Trifolium differs from other legumes in that the suspensor is short and has a broad attachment. This arrangement was observed in zygotic embryos of T. rubens and in many somatic embryos. However, a continuum of somatic embryogenesis was observed where some young embryos had a Trifolium suspensor-like arrangement while others were attached to a long narrow suspensor-like structure more characteristic of Medicago.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039887
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  • 6
    Electronic Resource
    Electronic Resource
    Genotype-dependent whole plant regeneration from protoplasts of red clover (Trifolium pratense L.) (1989)
    Springer
    Plant cell, tissue and organ culture 19 (1989), S. 113-127 
    Details
    ISSN: 1573-5044
    Keywords: somatic embryogenesis ; plant regeneration ; protoplasts ; Trifolium pratense ; red clover ; protoclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts are useful for subcellular studies, in vitro selection, somatic hybridization and transformation. Whole plant regeneration from protoplasts is a prerequisite to producing altered crop plants using these methods. Whole plant regeneration was achieved from leaf- and suspension culture-derived protoplasts of T. pratense. Regeneration was most dependent upon identifying genotypes with genetic capacity to regenerate. Additional factors that were used to select genotypes, but which proved to be less important, were a high rate of cell growth in culture and a high plating efficiency of protoplasts. One genotype was identified which had a regeneration response equivalent to that of T. rubens and which regenerated from both leaf- and suspension culture-derived protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035811
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  • 7
    Electronic Resource
    Electronic Resource
    Genetic male sterility in the pea (Pisum sativum L.): I. Inheritance, allelism and linkage (1988)
    Springer
    Euphytica 38 (1988), S. 165-174 
    Details
    ISSN: 1573-5060
    Keywords: Pisum sativum ; pea ; genetic male sterility ; genetics ; allelism ; linkage ; meiotic mutants ; microsporogenesis ; outcrossing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Relatively few male sterile (ms) genes have been reported in the pea despite description of numerous qualitative genes for other traits. This study was undertaken to characterize genetic male sterility in the pea. Male sterility may facilitate outcrossing because the pea is cleistogamous. Of fourteen lines carrying male sterility, tests of allelism revealed nine unique ms genes. According to F2 and F3 data, all ms genes segregated as single recessive genes. Linkage relations were determined for all but two. The genes appeared to be distributed randomly across the chromosomes. Two ms genes (ms-3 and ms-10) exhibited reduced female fertility in addition to male sterility. These mutants may have utility in increasing genetic recombination, particularly where hand hybridization is used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040188
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