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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Microchimica acta 106 (1992), S. 319-325 
    ISSN: 1436-5073
    Keywords: diphenhydramine ; flow-injection analysis (FIA) ; fluorometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A sensitive, rapid and simple flow injection procedure for the determination of diphenhydramine has been designed based on a fluorometric approach. An aqueous solution of diphenhydramine is injected into a carrierreagent stream containing Ce(IV) in dilute sulphuric acid and the fluorescence intensity of the Ce(III) produced is monitored. Chemical, FIA and instrumental variables were optimized. Analytical features of the method are: linear range 0.2–2 ppm, precision 0.7%, sample throughput 80/h. The influence of some foreign substances which can be found in typical pharmaceutical samples containing diphenhydramine was also investigated. The diphenhydramine content of a pharmaceutical preparation was determined.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1157
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Whether or not there are extensional detachment faults in the Alboran basement can be tested directly because a part of the Alboran Basin is now emerged. These detachments, related to crustal thinning beneath the Alboran Basin, occurred from the Aquitanian to Tortonian. The resulting extensional geometries can be described in general terms. During the Serravalian a considerable southwest extension of the basin took place, accompanied by south-southeast extension in the northern Gibraltar Arc. Other detachments affected by Serravalian extension can be found. The spreading of the Alboran was nearly coeval with roughly westward migration of the Gibraltar mountain front.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of environmental contamination and toxicology 52 (1994), S. 163-170 
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2048
    Keywords: Acylcyclohexanedione ; Epicotyl elongation ; Gibberellin (biosynthesis) ; Inhibitor (LAB 198 999) ; Vigna (epicotyl, gibberellin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of LAB 198 999 [3,5-dioxo-4-butyryl-cyclohexane carboxylic acid ethyl ester; a new plant growth retardant which competitively inhibits 2-oxoglutarate-dependent gibberellin (GA) dioxygenases] on elongation and in-vivo [3H]GA1 and [3H]GA20 metabolism in cowpea (Vigna sinensis L. cv Blackeye pea No. 5) epicotyls has been investigated. Gibberellins and LAB 198 999 were injected into the epicotyl at 25–30 mm from the apex. In intact seedlings, epicotyl elongation was inhibited by LAB 198 999 (25 μg · epicotyl-1), and the inhibition was counteracted by GA1 but not by GA20. In contrast to intact seedlings, the inhibitor enhanced epicotyl elongation in de-bladed seedlings and expiants, in the latter case proportionally to the amount of inhibitor applied (up to 50 μg · epicotyl-1), but not in explants made from paclobutrazol-treated seedlings. The inhibitor also enhanced dramatically the elongation induced in paclobutrazol-treated expiants by GA1, but not by GA20. The promotive effect of LAB 198 999 was associated with increased contents of GA1 and GA8 in the growing region of the epicotyl, indicating a dependence on endogenous GAs. The effect of LAB 198999 decreased progressively with the age of the seedlings, probably as a consequence of a decreased level of GAs in the epicotyl. Gibberellin substrates and metabolites in the growing region of the epicotyl (upper 20 mm) were fractionated and identified tentatively by high-performance liquid chromatography and radiocounting using a homogeneous on-line radioactivity detector. The metabolism of [3H]GA1(t) (tentative) to [3H]GA8(t), and that of [3H]GA20(t) to [3H]GA1(t) and [3H]GA29(t) in the epicotyl were blocked by LAB 198 999, that of the former more efficiently than the latter. The results presented support the hypothesis that GA1 is the active GA controlling elongation of cowpea epicotyls. They also show that both the promotion of epicotyl elongation in explants and the enhancement of the effect of exogenous GA1 by LAB 198 999 are the result of the inhibitor blocking the in-vivo 2β-hydroxylation of GA1.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 500-504 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Homoveratric acid (HVA) degradation was observed in cultures of Pleurotus eryngii lacking lignin peroxidase (LiP) activity. Extracellular enzymes seemed responsible for this transformation, and the lack of activity after ultrafiltration of the culture liquid suggests that the presence of some low-molecular-size compounds is required. This hypothesis is supported by rapid HVA transformation after addition of the synthetic laccase substrate 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) to the ultrafiltered liquid. HVA transformation by the extracellular enzymes from P. eryngii takes place via Cα-Cβ breakdown and formation of veratryl alcohol, which is further transformed into veratraldehyde. The same major compounds were found during HVA transformation by LiP from Phanerochaete chrysosporium, but this reaction was not stimulated by ABTS. Although the involvement of other enzymes cannot be ruled out, purified laccase from Pleurotus eryngii caused the same HVA transformation pattern in presence of ABTS. Moreover, veratryl alcohol oxidation by P. eryngii laccase was demonstrated in the presence of ABTS. These results suggest that enzymatic systems lacking LiP could be responsible for natural degradation of lignin.
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  • 6
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The kinetics of straw solid-state fermentation (SSF) with Trametes versicolor and Pleurotus ostreatus was investigated to characterize the delignification processes by these white-rot fungi. Two successive phases could be defined during straw transformation, characterized by changes in respiratory activity, changes in lignin and polysaccharide content and composition, increase in in-vitro digestibility, and enzymatic activities produced by the fungi. Lignin composition was analysed after CuO alkaline degradation, and decreases in syringyl/guaiacyl and syringyl/p-hydroxyphenyl ratios and cinnamic acid content were observed during the fungal treatment. An increase in the phenolic acid yield, revealing fungal degradation of side-chains in lignin, was produced by P. ostreatus. The highest xylanase level was produced by P. ostreatus, and exocellulase activity was nearly absent from straw treated with this fungus. Lactase activity was found in straw treated with both fungi, but lignin peroxidase was only detected during the initial phase of straw transformation with T. versicolor. High levels of H2O2-producing aryl-alcohol oxidase occurred throughout the straw SSF with P. ostreatus.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The effect of benzyl alcohol, benzaldehyde and benzoic acid on the production of extracellular hydrogen peroxide (H2O2) by the ligninolytic fungus Pleurotus eryngii was investigated. It was found that an equilibrium between oxidative and reductive reactions of these compounds is established, leading to the continuous production of H2O2. A multienzymatic cyclic system is proposed in which H2O2 is produced extracellularly by the action of aryl-alcohol oxidase on benzyl alcohol, the most abundant compound after redox reactions, and to a lower extent on benzaldehyde. The oxidation products of these reactions, benzaldehyde and benzoic acid, are reduced by intracellular dehydrogenases.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 32 (1990), S. 465-469 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Production of extracellular hydrogen peroxide by fungal oxidases is been investigated as a requirement for lignin degradation. Aryl-alcohol oxidase activity is described in extracellular liquid and mycelium ofPleurotus eryngii and studied under non-limiting nitrogen conditions. This aryl-alcohol oxidase catalyses conversion of primary aromatic alcohols to the corresponding aldehydes and H2O2, showing no activity with aliphatic and secondary aromatic alcohols. The enzyme is stable at pH 4.0–9.0, has maximal activity at 45°–50°C and pH 6.0–6.5, is inhibited by Ag+, Pb2+ and NaN3, and has aK m of 1.2 mM using veratryl alcohol as substrate. A single protein band with aryl-alcohol oxidase activity was found in zymograms of extracellular and intracellular crude enzyme preparations fromP. eryngii.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The effect of benzyl alcohol, benzaldehyde and benzoic acid on the production of extracellular hydrogen peroxide (H2O2) by the ligninolytic fungusPleurotus eryngii was investigated. It was found that an equilibrium between oxidative and reductive reactions of these compounds is established, leading to the continuous production of H2O2. A multienzymatic cyclic system is proposed in which H2O2 is produced extracellularly by the action of aryl-alcohol oxidase on benzyl alcohol, the most abundant compound after redox reactions, and to a lower extent on benzaldehyde. The oxidation products of these reactions, benzaldehyde and benzoic acid, are reduced by intracellular dehydrogenases.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 487-493 
    ISSN: 1432-0614
    Keywords: Key words Limonin ; Debittering ; Immobilization ; Rhodococcus fascians ; Continuous Stirred Tank Reactor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Limonin can be effectively degraded by Rhodococcus fascians cells. These bacteria can be entrapped in κ-carrageenan, and used in a continuous stirred tank reactor to degrade limonin in a continuous process. The effects of temperature, limonin concentration, dilution rate, and aeration on the reactor behaviour have been tested, and the results correlated with changes in limonin conversion, substrate degradation rate, and free and immobilized biomass. Results showed that the immobilized cells were able to debitter limonin-containing media and the immobilized biomass was quite stable throughout the operational conditions tested. A population of free biomass was present in the reactor, the quantity of which was dependent on dilution rate. The immobilized bacteria increased its limonin-degrading capability when the substrate concentration was increased. The aeration was not strictly necessary for limonin degradation. Additionally, the immobilized cells were active and stable for more than 2 months of continuous operation, and were able to recover their limonin-degrading capability when used intermittently. Finally, none of the main components of a juice was noticeably altered during limonin degradation, so the reactor response was good enough to consider its application.
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