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  • 1
    Electronic Resource
    Electronic Resource
    Chilean potato germplasm with resistance to potato viruses Y and S (1993)
    Springer
    Euphytica 69 (1993), S. 135-140 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ssp. tuberosum ; Chilean potato germplasm ; resistance to potato viruses Y and S
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four clones of the Chilota Potato Collection from the germplasm bank of the Universidad Austral de Chile, with potential combined resistance to PVX, PVY and PVS, were mechanically inoculated with these viruses in order to demonstrate wether they were truly resistant or not. Virus coat protein in the inoculated clones was determined by means of NCM-ELISA, DAS-ELISA, Western Blot and NASH. Two of the four inoculated clones were resistant to both PVY and PVS. This information will allow the utilization of these two clones in plant breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021737
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Two related, low-temperature-induced genes from Brassica napus are homologous to the human tumour bbc1 (breast basic conserved) gene (1993)
    Springer
    Plant molecular biology 23 (1993), S. 1211-1221 
    Details
    ISSN: 1573-5028
    Keywords: Brassica napus ; cold acclimation ; gene isolation ; human tumour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to identify genes involved in cold acclimation, we have constructed a cDNA library from Brassica napus (cv. Samouraï) cold-acclimated etiolated seedlings. By differential screening, a cDNA clone named pBnC24 (Brassica napus Cold), corresponding to a new cold-inducible plant gene, was isolated. Northern blot hybridizations using total RNA from acclimated and unacclimated seedlings confirmed that BnC24 represents a cold-regulated gene. In contrast with a number of cold-inducible plant genes, BnC24 does not seem to be responsive to abscisic acid (ABA). In addition, further screening of the ‘cold-acclimated’ cDNA library using pBnC24 cDNA as a probe, allowed the isolation of a second type of homologous cDNA. Sequence analysis showed that the two BnC24 genes encode basic 24 kDa proteins, which are highly hydrophilic and rich in alanine, lysine and arginine. The nucleotide and deduced amino acid sequences of these clones do not show any homology with other previously described cold-induced plants genes. However they have strong homology with a recently discovered human tumour gene, bbc1 (breast basic conserved), which seems to be highly conserved in eukaryotes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042354
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  • 3
    Electronic Resource
    Electronic Resource
    Nonsense suppression in thymine-requiring strains of Escherichia coli is a consequence of altered folate metabolism (1993)
    Springer
    Molecular genetics and genomics 238 (1993), S. 218-224 
    Details
    ISSN: 1617-4623
    Keywords: Thy−phenotype ; Suppression ; Folate levels ; Dihydrofolate ; Translational accuracy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thymine-requiring strains of Escherichia coli suppress nonsense and frameshift mutants of T4 phage. We proposed that these mutants make errors during translation because of an imbalance in folate metabolism. A thymine-requiring strain grown under suppressing conditions has elevated levels of reduced folates. We tested the effect of either mutational blocks or the inhibition of various steps in folate biosynthesis on suppression. Conditions which prevent the accumulation of 5-methyl tetrahydrofolate inhibit suppression, suggesting that elevated levels of this folate are required for suppression. Furthermore, conditions that result in an accumulation in dihydrofolate inhibit suppression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279550
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