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  • Springer  (116)
  • 1995-1999  (116)
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  • 1
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phylogenetic relationships among the seven species of deep-sea giant clams Calyptogena (Bivalvia: Vesicomyidae) collected around Japan were examined using parts of nucleotide sequences of mitochondrial genes for cytochrome oxidase I (COI) and cytochrome oxidase III (COIII) and the encoded amino acid sequences. The seven species were C. soyoae (Sagami Bay), C. fausta (Suruga Bay), C. kaikoi (Nankai Trough), C. nautilei (Nankai Trough), C. phaseoliformis (Japan Trench), C. solidissima (Minami-Ensei Knoll, Okinawa Trough) and Calyptogena sp. (Iheya Ridge, Okinawa Trough). A clear phylogenetic split was observed between one group of three species (C. kaikoi, C. phaseoliformis and C. fausta) and the remaining species. This clustering corresponds to the two previously described subgenera within the genus Calyptogena (Calyptogean and Ectenagena) with the exception of the placement of C. nautilei, which had been placed in the subgenus Ectenagena. Genetic distances between two haplotypes of C. soyoae were 0.043 for the COI region and 0.055 for the COIII region, and three amino acid substitutions were detected with the COIII region. Calyptogena sp. from the Iheya Ridge could be distinguished from one of the two haplotypes (type A) of C. soyoae by only a single nucleotide substitution, a result that suggests that Calyptogena sp. of the Iheya Ridge diverged from C. soyoae after the two haplotypes had diverged, and it is now isolated from C. soyoae in Sagami Bay.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Marine biology 126 (1996), S. 585-590 
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The pilchards (genus Sardinops), one of the major components of the world's harvest of fishes, is distributed sporadically in the temperate zone on a global scale. Mitochondrial DNA variation in 95 pilchards from nine worldwide localities in five current systems was examined using ten restriction enzymes. Although several opinions have been proposed on the global population structure of the genus Sardinops, our results clearly rejected the hypothesis of gene flow among geographically distant populations on a global scale. Results indicate that the Japanese pilchards and the Australian and South African pilchards were derived from the eastern Pacific populations, following the speciation of Sardinops in the Pacific, after the formation of the Isthmus of Panama. An intermixing of the northeastern and southeastern Pacific populations during the Pleistocene glacial maxima is suggested from their genetic affinities.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 69 (1999), S. S587 
    ISSN: 1432-0630
    Keywords: PACS: 81.15.Fg; 73.61.-r; 77.22.Ch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract. In this study, the pulsed laser ablation of RuO2 and SrRuO3 (SRO) is investigated by observing the fluorescence from excited atoms in the plume by using a framing streak camera. Vaporization, phase explosion and boiling are suggested to play the main roles in the processes for the interaction between the laser beam and target. Collisions and adiabatic expansion are also suggested before particles move forward with shifted Maxwellian spatial distribution. In O2 pressure, numerous collisions between fast and slow atoms occur and result in the exchange of speeds. The structural and electrical (conductivity and work-function) properties of RuO2 and SRO thin films are measured. Epitaxial SRO growth was obtained at growth temperatures down to 350 °C. Ferroelectric and high- dielectric thin-film capacitors with RuO2 or SRO thin film electrode are also studied.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of sol gel science and technology 16 (1999), S. 165-171 
    ISSN: 1573-4846
    Keywords: barium titanate ; sol-gel processing using high-concentration alkoxide solution ; optical measurements ; absorption edge ; crystallite size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Transparent barium titanate (BaTiO3) wet gels synthesized by sol-gel processing using high-concentration Ba,Ti-alkoxide solution (1.0 mol/l) were subjected to optical measurements, X-ray diffraction analysis and transmission electron microscope observations. A systematic shift of the optical absorption edge was observed for the transparent BaTiO3 wet gels shrinking during aging at 30 and 50°C, while no shift was observed for the wet gels aged at 5°C. The crystallinity and crystallite size of the gels increased with increasing aging time and temperature. The shift of the optical absorption edge of the BaTiO3 gels during aging was found to have a close relation to an increase in the crystallite size of the gels.
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  • 5
    ISSN: 1435-1536
    Keywords: Key words Cross-linking of polymer layer ; composite particles ; poly(maleic anhydride-styrene) ; monodisperse colloidal silica ; surface modification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary  The cross-linkings of the surface polymer layer on mono disperse, poly(maleic anhydride-styrene)-modified silica particles by the reaction with diisocyanate were studied. The extent of cross-linking was estimated by the weight decrease by immersing the particles in the buffer solution of pH 2.0, 4.0 and 9.0 at a room temperature for 24 h. The reaction of the polymer-modified silica with 1,6-diisocyanatohexane afforded relatively stable composite particles which lost less than 5 wt% of the polymer in aqueous solution in the pH range 2.0–9.0. The diisocyanate was a preferable cross-linker to 2,4-diisocyanatotoluene in terms of stability in acidic or basic aqueous solution. The flexibility of the cross-linker molecule possibly plays an important role in the cross-linking reaction. The carboxyl and amino groups were formed by treating the cross-linked composite particles with diluted HCl solution; 5–6 and 0.5–1.1 μmol g-1, respectively. The cross-linked composite particles exhibited the characteristic property of ζ-potential, −44 to −47 mV and −102 to −107 mV in a neutral aqueous solution and ethanol, respectively.
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  • 6
    ISSN: 1617-4623
    Keywords: Key wordsAspergillus  ;  Taka-amylase A  ;  CCAAT  ;  Nuclear protein  ;  Footprint
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using AnCP (Aspergillus nidulans CCAAT-binding protein) as a CCAAT-specific binding factor model, the possibility that one factor is able to recognize CCAAT sequences in several different genes in A.␣nidulans was examined. DNase I protection analysis showed that AnCP specifically bound to CCAAT sequence-containing regions comprising 21 to 36 bp of the taa, amdS and gatA genes. Furthermore, replacement of the CCAAT sequence with CGTAA was found to abolish the binding of AnCP and to have an inhibitory effect on taa promoter activity. This clearly demonstrates a positive function of the CCAAT element. However, amylase was induced by starch and repressed by glucose in a CCAAT-box disruptant, as in wild-type cells.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1617-4623
    Keywords: Key wordsDrosophila melanogaster  ;  Small GTPases rab  ;  rho  ;  rac
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated eight genes from Drosophila, small GTPases. They can be classified into three rab family genes (Drab2, Drab5, Drab11) and five rho family genes (Drac1a, Drac1b, Drac3, Dcdc42, DrhoA). While Drac3 is a novel type of rac gene, others are homologues of known mammalian genes for small GTPases. Northern blot analyses showed that all the genes are expressed throughout all developmental stages from embryo to adult. In situ hybridization to embryos revealed that Drab2, Drac1b, and Drac3 are highly expressed in the nervous system, in the trunk mesoderm, and in the cephalic mesoderm, respectively. Since hemocytes are derived from the cephalic mesoderm, we carried out double stainings using a hemocyte marker – anti-peroxidasin antibody – and Drac3 in situ hybridization. We found that Drac3 is expressed in hemocyte precursor cells. In the Drac3 deficiency embryos, the hemocyte precursor cells start to differentiate normally, but never develop into mature hemocytes, indicating that Drac3 is essential for their maturation. The DrhoA and Dcdc42 genes complemented S. cerevisiae rho1 and cdc42 mutations in the same manner as human rhoA and CDC42, respectively. These results suggest functional similarity between Drosophila and mammalian small GTPase genes.
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  • 8
    ISSN: 1617-4623
    Keywords: Key wordsAspergillus ; CCAAT ; HapC ; Hap complex ; DNA binding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Aspergillus nidulans hapC gene was expressed as a fusion protein with MalE or glutathione-S-transferase (GST) in Escherichia coli, and used for the purification of HapC and the preparation of anti-HapC antiserum. The CCAAT-binding factor AnCP/AnCF contains a component with an approximate molecular mass of 32 kDa that cross-reacts with the antibody. The MalE-HapC fusion protein was able to replace authentic HapC in AnCP when incubated under appropriate conditions. Furthermore, reconstitution experiments with recombinant HapC, yHAP2 and yHAP5 polypeptides showed that all three polypeptides were required for the assembly of a complex capable of binding to CCAAT-containing taaG2 promoter DNA. The relationship between AnCP/AnCF and the Saccharomyces cerevisiae HAP complex is discussed.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of environmental contamination and toxicology 54 (1995), S. 440-443 
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of environmental contamination and toxicology 57 (1996), S. 556 -559 
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
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