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  • Springer  (165)
  • 2000-2004  (162)
  • 1920-1924
  • 1890-1899
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  • 1
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    Unknown
    Drying dissipative structures of Chinese black ink on a cover glass and in a dish (2004)
    Springer
    Details
    Publication Date: 2004-04-01
    Print ISSN: 0372-820X
    Electronic ISSN: 1435-1536
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Published by Springer
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  • 2
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    Dissipative structures formed in the course of drying the colloidal crystals of monodispersed polystyrene spheres on a cover glass (2002)
    Springer
    Details
    Publication Date: 2002-11-01
    Print ISSN: 0372-820X
    Electronic ISSN: 1435-1536
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Published by Springer
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  • 3
    Electronic Resource
    Electronic Resource
    Mosaic horizontal distributions of three species of copepods in the subarctic Pacific during spring (2000)
    Springer
    Marine biology 137 (2000), S. 683-689 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Continuous abundance estimates (510 m resolution) of the copepods Neocalanus cristatus, N. flemingeri and Metridia pacifica were obtained with an electronic particle counter along cruise tracks in the subarctic western North Pacific in spring. For all three species, the number of patches decreased exponentially with increasing patch size. Most patches (63 to 83%) were dominated by one species, and patches of the same species more closely spaced than patches of different species. The patches of M. pacifica tended to coexist with those of N. cristatus. In contrast, patches of N. flemingeri rarely co-occurred with those of other copepods. These patterns were more clearly observed in fine-scale observations with sampling intervals of 〈31 m. Coherence analysis of copepod species pairs showed no characteristic scale at 2 to 50 km wave lengths. At shorter wave lengths (〈2 km), frequent positive correlations were observed between N. cristatus and M. pacifica. Thus, the distribution of copepods appears to be a mosaic assemblage of patches of each copepod species. These results suggest that copepods have a mechanism to form species-specific aggregations, and the aggregation and segregation processes are maintained at a scale of 〈2 km.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002270000338
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  • 4
    Electronic Resource
    Electronic Resource
    A novel truncated isoform of the mannose-binding lectin-associated serine protease (MASP) from the common carp (Cyprinus carpio) (2000)
    Springer
    Immunogenetics 51 (2000), S. 193-200 
    Details
    ISSN: 1432-1211
    Keywords: Key words MASP ; Lectin pathway ; Complement ; Truncated form ; Alternative polyadenylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The mannose-binding lectin (MBL) and MBL-associated serine proteases (MASPs) play crucial roles in activation of the lectin pathway of the complement system. Mammals and Xenopus possess two distinct MASPs, MASP1 and MASP2, with different substrate specificity. Recently, a truncated form named MAp19 or sMAP, composed of N-terminal C1r/C1s/Uegf/bone morphogenetic protein (CUB)-1 and epidermal growth factor domains of MASP2, has been shown to be generated by alternative polyadenylation and splicing from the MASP2 gene. In the present study, we isolated cDNA encoding a novel MASP-related protein, designated MRP, from carp. MRP is distinct from MAp19/sMAP in containing two additional domains, CUB-2 and short concensus repeat (SCR)-1, followed by a unique C-terminal 21 amino acids, but resembles it by also lacking the serine protease domain, suggesting that carp MRP is a functional homologue of human MAp19/sMAP. Analyses of polymerase chain reaction (PCR)-amplified carp genomic DNA, from CUB-2 to SCR-2 of MASP, indicated that carp possess duplicated MASP genes, designated MASP-A and MASP-B, both of which contain an exon encoding the MRP-specific C-terminal stretch between the exons coding for SCR-1 and SCR-2 domains. Reverse transcription-PCR analysis showed that both MASP genes of carp produce the two MASP isoforms, MASP and MRP, through alternative polyadenylation and splicing. The conservation of MASP isoforms that lack the catalytic domain in both carp and human implies that they meet an essential requirement in the MBL-MASP complex of the lectin pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050031
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  • 5
    Electronic Resource
    Electronic Resource
    Importance of GAD65 peptides and I-Ag7 in the development of insulitis in nonobese diabetic mice (2000)
    Springer
    Immunogenetics 51 (2000), S. 538-545 
    Details
    ISSN: 1432-1211
    Keywords: Key words NOD mouse ; GAD65 ; I-Ag7 ; Peptide ; Vaccine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Insulin-dependent diabetes mellitus (IDDM) develops in nonobese diabetic (NOD) mice through the destruction of the B cells in pancreatic Langerhans islets by islet autoantigen-specific T cells. The islet autoantigen glutamic acid decarboxylase 65 (GAD65) is thought to be a major target autoantigen in IDDM. In the present report, we established GAD65-specific T-cell clones using overlapping peptides that cover the amino acid sequences of mouse GAD65. T-cell epitopes of GAD65 were characterized by proliferation and binding assays using various analogue peptides and wild-type or mutant I-Ag7 transfectants. The efficacy of the peptide vaccine in IDDM was determined by administering T-cell epitope peptides to NOD mice and evaluating the histopathology of their insulitis. We obtained two types of T-cell clone, one specific for peptide p316–335 and another specific for p531–545 of GAD65. The p531–545 site has already been identified, but we report the p316–335 site for the first time. T-cell clones recognized those peptides in the wild-type I-Ag7 but not in the mutant I-Ag7 in which the serine at position 57 of the β-chain was replaced by an aspartic acid. Both the p316–335 and p531–545 peptides bound weakly to I-Ag7. Some peptides with amino acid substitutions had antagonistic activity, and administration of a large amount of wild-type peptide reduced the severity of insulitis in NOD mice. Our results suggest that peptide vaccine therapy may be useful in autoimmune diseases, including IDDM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510000173
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  • 6
    Electronic Resource
    Electronic Resource
    Purification and characterization of isoamyl acetate-hydrolyzing esterase encoded by the IAH1 gene of Saccharomyces cerevisiae from a recombinant Escherichia coli (2000)
    Springer
    Applied microbiology and biotechnology 53 (2000), S. 596-600 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The IAH1 gene of Saccharomyces cerevisiae encodes an esterase that preferentially acts on isoamyl acetate; however, the enzyme has not yet been completely purified from the yeast S. cerevisiae. We constructed the IAH1 gene expression system in Escherichia coli, and purified the IAH1 gene product (Iah1p). The amount of Iah1p produced by recombinant E. coli was more than 40% of total cellular proteins. The molecular size of Iah1p was 28 kDa by SDS-polyacrylamide gel electrophoresis. Judging from the molecular weight estimation by gel filtration of purified Iah1p, the enzyme was thought to be a homodimer. The K m values for isoamyl acetate and isobutyl acetate were 40.3 mM and 15.3 mM, respectively. The enzyme activity was inhibited by Hg2+, p-chloromercuribenzoate, and diisopropylfluorophosphate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051662
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  • 7
    Electronic Resource
    Electronic Resource
    Immobilization of Photosynthetic Pigments into Silica-Surfactant Nanocomposite Films (2000)
    Springer
    Journal of sol gel science and technology 19 (2000), S. 543-547 
    Details
    ISSN: 1573-4846
    Keywords: silica-surfactant nanocomposite film ; photosynthetic pigment ; immobilization ; chlorophyll ; pheophytin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Highly transparent silica-surfactant nanocomposite films containing photosynthetic pigments have been successfully formed through the solubilization of chlorophyll a (Chl a) into surfactant micelles. The UV-vis absorption spectra indicated that a large amount of Chl a were transformed into pheophytin a in the films. These photosynthetic pigments were well dispersed in the surfactant assemblies and their chlorin rings were exposed to the surface of silica layers. Even under an air atmosphere, the photostability of immobilized pigments was largely improved in comparison with that in a homogeneous Chl a solution. Because both Chl a and pheophytin a molecules are effective for the photosensitive charge separation, the present film system is very suitable for heterogeneous immobilizing media for photosynthetic pigments from the viewpoint of in vitro biomimetic devices for solar energy conversion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008740723019
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  • 8
    Electronic Resource
    Electronic Resource
    Controlling Factors for the Conversion of Trifluoroacetate Sols into Thin Metal Fluoride Coatings (2000)
    Springer
    Journal of sol gel science and technology 19 (2000), S. 311-314 
    Details
    ISSN: 1573-4846
    Keywords: fluorides ; thin film ; trifluoroacetic acid ; anti-reflective coatings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Metal fluoride films were prepared from trifluoroacetate sols by a spin-coating method. Effects of preparation conditions on the deposition of fluoride films have been examined in order to control the thickness and the refractive index of the films. It was suggested that the fluoride was formed by the thermal decomposition of the trifluoroacetate sols generating gaseous phases such as (CF3CO)2O, CO and CO2. This reaction mechanism seemed to largely effect the deposition of the fluoride films in terms of the formation of pores. Characteristics of the fluoride films could be changed by varying the heat treatment. The refractive index of the MgF2 films decreased with increasing heating temperature, which might result from the increased porosity of the films. Drying the film before the heat treatments was effective in the control of the refractive index.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008729531254
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  • 9
    Electronic Resource
    Electronic Resource
    Coexistence of drosophilid flies: Aggregation, patch size diversity and parasitism (2000)
    Springer
    Ecological research 15 (2000), S. 93-100 
    Details
    ISSN: 1440-1703
    Keywords: aggregation ; coexistence ; Drosophila ; parasitism ; patch size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: We carried out field experiments to investigate the coexistence of Drosophila species in domestic and forest areas on the basis of the aggregation model. Three cosmopolitan species Drosophila simulans Sturtevant, Drosophila melanogaster Meigen and Drosophila immigrans Sturtevant, and a native species, Drosophila auraria Peng, emerged abundantly from banana placed at the domestic station, while Drosophila immigrans and five native species, Drosophila lutescens Okada, Drosophila rufa Kikkawa and Peng, Drosophila bizonata Kikkawa and Peng, Drosophila sternopleuralis Okada and Kurokawa and Scaptodrosophila coracina (Kikkawa and Peng), were abundant at the forest station. The present analysis suggests that their coexistence was facilitated by the aggregation mechanism. In the cosmopolitan species, the density of individuals that emerged from patches increased with the increase of patch size, but the relationship between fly density and patch size was not clear in the native species. This difference in distribution patterns between the cosmopolitan and native species is likely to be due to the difference in the female visiting behavior. In the present analysis, however, it was not clear whether patch size diversity facilitated their coexistence or not. The effect of patch size diversity may have been masked, because the effect of aggregation was more prominent. The rate of parasitism by wasps was high in October at the domestic station, and in May and June at the forest station. The present result suggests that the rate of parasitism was density-dependent, at least at the domestic station, and therefore parasitism facilitates the coexistence of drosophilid species in domestic areas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1440-1703.2000.00328.x
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  • 10
    Electronic Resource
    Electronic Resource
    Interactive mesh dragging with an adaptive remeshing technique (2000)
    Springer
    The visual computer 16 (2000), S. 159-176 
    Details
    ISSN: 1432-2315
    Keywords: Key words: Mesh editing – Remeshing – Free-form modelling – Geometric modeling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003710050205
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