Publikationsdatum:
2015-05-21
Beschreibung:
:Background: Chromatin structure is implicated in regulating gene transcription in stressresponse. Transcription factors, transferases, and deacetylases, such as Msn2, Set1, and Snf1,have been identified as key regulators in stress response. Here, we reported the dynamics ofnucleosome occupancy, Htz1 deposition, and histone H3 lysine 4 dimethylation (H3K4me2)and histone H3 lysine 79 trimethylation (H3K79me3) in Saccharomyces cerevisiae underoleate stress.Results: Our results indicated that citrate cycle-associated genes are enhanced and ribosomegenes are repressed during the glucose-oleate shift. Importantly, Htz1 acts as a sensor foroleate stress. High-throughput ChIP-chip analysis showed that Htz1 has redistributed acrossthe genome during oleate stress. The number of Htz1-bound genes increases with stress, andthe number of Htz1-bound ribosome genes decreases with stress. The dynamics of Htz1 andH3K79me3 around transcription factor-binding sites correlate with transcriptional changes.Moreover, we found that nucleosome dynamics are coupled with Htz1 binding changes uponstress. In unstressed conditions (2% glucose), nucleosome occupancy is comparable betweenHtz1-bound genes and Htz1-depleted genes; in stressed conditions (0.2% oleate for 8 hours),the nucleosome occupancy of Htz1-depleted genes is significantly lower than that ofHtz1-bound genes. We also found that Msn2 acts an important role in response to the oleatestress and Htz1 is dynamic in Msn2-target genes.Conclusions: Htz1 senses the oleate stress and undergoes a global redistribution, and thischange couples dynamics of nucleosome occupancy. Our analysis suggests that Htz1 andnucleosome dynamics change in response to oleate stress.
Print ISSN:
0144-8463
Digitale ISSN:
1573-4935
Thema:
Biologie
,
Chemie und Pharmazie
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