ALBERT

Description: In prokaryotes, genome size is associated with metabolic versatility, regulatory complexity, effective population size, and horizontal transfer rates. We therefore analyzed the covariation of genome size and operon conservation to assess the evolutionary models of operon formation and maintenance. In agreement with previous results, intraoperonic pairs of essential and of highly expressed genes are more conserved. Interestingly, intraoperonic pairs of genes are also more conserved when they encode proteins at similar cell concentrations, suggesting a role of cotranscription in diminishing the cost of waste and shortfall in gene expression. Larger genomes have fewer and smaller operons that are also less conserved. Importantly, lower conservation in larger genomes was observed for all classes of operons in terms of gene expression, essentiality, and balanced protein concentration. We reached very similar conclusions in independent analyses of three major bacterial clades (α- and β-Proteobacteria and Firmicutes). Operon conservation is inversely correlated to the abundance of transcription factors in the genome when controlled for genome size. This suggests a negative association between the complexity of genetic networks and operon conservation. These results show that genome size and/or its proxies are key determinants of the intensity of natural selection for operon organization. Our data fit better the evolutionary models based on the advantage of coregulation than those based on genetic linkage or stochastic gene expression. We suggest that larger genomes with highly complex genetic networks and many transcription factors endure weaker selection for operons than smaller genomes with fewer alternative tools for genetic regulation.

Description: Stellar intensity interferometers will achieve stellar imaging with a tenth of a milli-arcsecond resolution in the optical band by taking advantage of the large light collecting area and broad range of intertelescope distances offered by future gamma-ray Air Cherenkov Telescope (ACT) arrays. Up to now, studies characterizing the capabilities of intensity interferometers using ACTs have not accounted for realistic effects such as telescope mirror extension, detailed photodetector time response, excess noise and night sky contamination. In this paper, we present the semiclassical quantum optics Monte Carlo simulation we developed in order to investigate these experimental limitations. In order to validate the simulation algorithm, we compare our first results to models for sensitivity and signal degradation resulting from mirror extension, pulse shape, detector excess noise and night sky contamination.

Description: Optical interferometry has been successful at achieving milliarcsecond resolution on bright stars. Imaging performance can improve greatly by increasing the number of baselines, which has motivated proposals to build large (~100 m) optical interferometers with tens to hundreds of telescopes. It is also desirable to adaptively correct atmospheric turbulence to obtain direct phased images of astrophysical sources. When a natural guide star is not available, we investigate the feasibility of using a modified laser-guide-star technique that is suitable for large diluted apertures. The method consists of using subsets of apertures to create an array of artificial stars in the sodium layer and collecting back-scattered light with the same subapertures. We present some numerical and laboratory simulations that quantify the requirements and sensitivity of the technique.

Description: Future large arrays of telescopes, used as intensity interferometers, can be used to image the surfaces of stars with unprecedented angular resolution. Fast-rotating, hot stars are particularly attractive targets for intensity interferometry since shorter (blue) wavelength observations do not pose additional challenges. Starting from realistic surface brightness simulations of fast-rotating stars, we discuss the capabilities of future intensity interferometers for imaging effects such as gravity darkening and rotational deformation. We find that two-telescope intensity correlation data allow reasonably good imaging of these phenomena, but can be improved with additional higher order (e.g. three-telescope) correlation data, which contain some Fourier phase information.