ALBERT

Description: We present MUSE observations of the debated ultrafaint stellar system Crater. We spectroscopically confirm 26 member stars of this system via radial velocity measurements. We derive the systematic instrumental velocity uncertainty of MUSE spectra to be 2.27 km s – 1 . This new data set increases the confirmed member stars of Crater by a factor of 3. One out of three bright blue stars and a fainter blue star just above the main-sequence turn-off are also found to be likely members of the system. The observations reveal that Crater has a systemic radial velocity of $v_{\rm sys}=148.18^{\rm +1.08}_{\rm -1.15}\rm \,km\,s^{-1}$ , whereas the most likely velocity dispersion of this system is $\sigma _{\rm v}=2.04^{\rm +2.19}_{\rm -1.06} \rm \,km\,s^{-1}$ . The total dynamical mass of the system, assuming dynamical equilibrium is then $M_{\rm tot}=1.50^{+4.9}_{-1.2}\times \ 10^{\rm 5}\,\mathrm{M}_{{\odot }}$ implying a mass-to-light ratio of M/L V = 8.52 $^{+28.0}_{-6.5}\, \,\mathrm{M}_{{\odot }}/\mathrm{L}_{{\odot }}$ , which is consistent with a purely baryonic stellar population within its errors and no significant evidence for the presence of dark matter was found. We also find evidence for a velocity gradient in the radial velocity distribution. We conclude that our findings strongly support that Crater is a faint intermediate-age outer halo globular cluster and not a dwarf galaxy.

Description: The cone photoreceptor cyclic nucleotide-gated (CNG) channel is essential for central and color vision and visual acuity. Mutations in the channel subunits CNGA3 and CNGB3 are associated with achromatopsia and cone dystrophy. We investigated the gene expression profiles in mouse retina with CNG channel deficiency using whole genome expression microarrays. As cones comprise only 2 to 3% of the total photoreceptor population in the wild-type mouse retina, the mouse lines with CNG channel deficiency on a cone-dominant background, i.e. Cnga3 –/– /Nrl –/– and Cngb3 –/– /Nrl –/– mice, were used in our study. Comparative data analysis revealed a total of 105 genes altered in Cnga3 –/– /Nrl –/– and 92 in Cngb3 –/– /Nrl –/– retinas, relative to Nrl –/– retinas, with 27 genes changed in both genotypes. The differentially expressed genes primarily encode proteins associated with cell signaling, cellular function maintenance and gene expression. Ingenuity pathway analysis (IPA) identified 26 and 9 canonical pathways in Cnga3 –/– /Nrl –/– and Cngb3 –/– /Nrl –/– retinas, respectively, with 6 pathways being shared. The shared pathways include phototransduction, cAMP/PKA-mediated signaling, endothelin signaling, and EIF2/endoplasmic reticulum (ER) stress, whereas the IL-1, CREB, and purine metabolism signaling were found to specifically associate with Cnga3 deficiency. Thus, CNG channel deficiency differentially regulates genes that affect cell processes such as phototransduction, cellular survival and gene expression, and such regulations play a crucial role(s) in the retinal adaptation to impaired cone phototransduction. Though lack of Cnga3 and Cngb3 shares many common pathways, deficiency of Cnga3 causes more significant alterations in gene expression. This work provides insights into how cones respond to impaired phototransduction at the gene expression levels.

Description: In recent years, a growing zoo of compact stellar systems (CSSs) have been found whose physical properties (mass, size, velocity dispersion) place them between classical globular clusters (GCs) and true galaxies, leading to debates about their nature. Here we present results using a so far underutilized discriminant, their stellar population properties. Based on new spectroscopy from 8–10m telescopes, we derive ages, metallicities, and [α/Fe] of 29 CSSs. These range from GCs with sizes of merely a few parsec to compact ellipticals (cEs) larger than M32. Together with a literature compilation, this provides a panoramic view of the stellar population characteristics of early-type systems. We find that the CSSs are predominantly more metal rich than typical galaxies at the same stellar mass. At high mass, the cEs depart from the mass–metallicity relation of massive early-type galaxies, which forms a continuous sequence with dwarf galaxies. At lower mass, the metallicity distribution of ultracompact dwarfs (UCDs) changes at a few times 10 7 M , which roughly coincides with the mass where luminosity function arguments previously suggested the GC population ends. The highest metallicities in CSSs are paralleled only by those of dwarf galaxy nuclei and the central parts of massive early types. These findings can be interpreted as CSSs previously being more massive and undergoing tidal interactions to obtain their current mass and compact size. Such an interpretation is supported by CSSs with direct evidence for tidal stripping, and by an examination of the CSS internal escape velocities.

Description: Alternation of generations, in which the haploid and diploid stages of the life cycle are each represented by multicellular forms that differ in their morphology, is a defining feature of the land plants (embryophytes). Anciently derived lineages of embryophytes grow predominately in the haploid gametophytic generation from apical cells that give rise to the photosynthetic body of the plant. More recently evolved plant lineages have multicellular shoot apical meristems (SAMs), and photosynthetic shoot development is restricted to the sporophyte generation. The molecular genetic basis for this evolutionary shift from gametophyte-dominant to sporophyte-dominant life cycles remains a major question in the study of land plant evolution. We used laser microdissection and next generation RNA sequencing to address whether angiosperm meristem patterning genes expressed in the sporophytic SAM of Zea mays are expressed in the gametophytic apical cells, or in the determinate sporophytes, of the model bryophytes Marchantia polymorpha and Physcomitrella patens . A wealth of upregulated genes involved in stem cell maintenance and organogenesis are identified in the maize SAM and in both the gametophytic apical cell and sporophyte of moss, but not in Marchantia . Significantly, meiosis-specific genetic programs are expressed in bryophyte sporophytes, long before the onset of sporogenesis. Our data suggest that this upregulated accumulation of meiotic gene transcripts suppresses indeterminate cell fate in the Physcomitrella sporophyte, and overrides the observed accumulation of meristem patterning genes. A model for the evolution of indeterminate growth in the sporophytic generation through the concerted selection of ancestral meristem gene programs from gametophyte-dominant lineages is proposed.

Description: Human noroviruses cause recurrent epidemics of gastroenteritis known to be dominated by the clinically important GII.4 genotype which recognizes human Secretor gene-dependent ABH histo-blood group antigens (HBGAs) as attachment factors. There is increasing evidence that GII.4 noroviruses have undergone evolutionary changes to recognize Lewis antigens and non-Secretor saliva. In this study, we have investigated the possibilities of the Lewis α1,3/α1,4 fucoses as mediators of binding of GII.4 noroviruses to Lewis antigens. The study was carried out using molecular dynamics simulations of Lewis type-1 and type-2 chain HBGAs in complex with VA387 P domain dimers in explicit water. Based on the computer simulations, we suggest the possibility of two receptor binding modes for Lewis HBGAs: the "Secretor pose" with the Secretor Fucα1,2 in the binding site and the "Lewis pose" with the Lewis Fucα1,3/α1,4 residues in the binding site. This was further supported by an extensive GlyVicinity analysis of the Protein Data Bank with respect to the occurrence of the Lewis and Secretor poses in complexes of Lewis antigens with lectins and antibodies as well as GII norovirus strains. The Lewis pose can also explain the interactions of GII.4 norovirus strains with Le x and SLe x structures. Moreover, the present model suggests binding of complex branched polysaccharides, with the Lewis antigens at the nonreducing end, to P domain dimers of GII.4 strains. Our results are relevant for understanding the evolution of norovirus binding specificities and for in silico design of future antiviral therapeutics.

Description: We use direct N -body calculations to study the evolution of the unusually extended outer halo globular cluster Palomar 4 (Pal 4) over its entire lifetime in order to reproduce its observed mass, half-light radius, velocity dispersion and mass function slope at different radii. We find that models evolving on circular orbits, and starting from a non-mass segregated, canonical initial mass function (IMF) can reproduce neither Pal 4s overall mass function slope nor the observed amount of mass segregation. Including either primordial mass segregation or initially flattened IMFs does not reproduce the observed amount of mass segregation and mass function flattening simultaneously. Unresolved binaries cannot reconcile this discrepancy either. We find that only models with both a flattened IMF and primordial segregation are able to fit the observations. The initial (i.e. after gas expulsion) mass and half-mass radius of Pal 4 in this case are about 57 000 M and 10 pc, respectively. This configuration is more extended than most globular clusters we observe, showing that the conditions under which Pal 4 formed must have been significantly different from that of the majority of globular clusters. We discuss possible scenarios for such an unusual configuration of Pal 4 in its early years.

Description: We present evidence for mass segregation in the outer halo globular cluster Palomar 14, which is intuitively unexpected since its present-day two-body relaxation time significantly exceeds the Hubble time. Based on archival Hubble Space Telescope imaging, we analyse the radial dependence of the stellar mass function in the cluster's inner 39.2 pc in the mass range of 0.53 ≤ m ≤ 0.80 M , ranging from the main-sequence turn-off down to a V -band magnitude of 27.1 mag. The mass function at different radii is well approximated by a power law and rises from a shallow slope of 0.6 ± 0.2 in the cluster's core to a slope of 1.6 ± 0.3 beyond 18.6 pc. This is seemingly in conflict with the finding by Beccari et al., who interpret the cluster's non-segregated population of (more massive) blue straggler stars, compared to (less massive) red giants and horizontal branch stars, as evidence that the cluster has not experienced dynamical segregation yet. We discuss how both results can be reconciled. Our findings indicate that the cluster was either primordially mass segregated and/or used to be significantly more compact in the past. For the latter case, we propose tidal shocks as the mechanism driving the cluster's expansion, which would imply that Palomar 14 is on a highly eccentric orbit. Conversely, if the cluster formed already extended and with primordial mass segregation, this could support an accretion origin of the cluster.

Description: Mammalian cell surfaces are decorated with a variety of glycan chains that orchestrate development and defense and are exploited by pathogens for cellular attachment and entry. While glycosidic linkages are, in principle, flexible, the conformational space that a given glycan can sample is subject to spatial and electrostatic restrictions imposed by its overall chemical structure. Here, we show how the glycan moiety of the GM1 ganglioside, a branched, monosialylated pentasaccharide that serves as a ligand for various proteins, undergoes differential conformational selection in its interactions with different lectins. Using STD NMR and X-ray crystallography, we found that the innate immune regulator complement Factor H (FH) binds a previously not reported GM1 conformation that is not compatible with the GM1-binding sites of other structurally characterized GM1-binding lectins such as the Simian Virus 40 (SV40) capsid. Molecular dynamics simulations of the free glycan in explicit solvent on the 10 μs timescale reveal that the FH-bound conformation nevertheless corresponds to a minimum in the Gibbs free energy plot. In contrast to the GM1 conformation recognized by SV40, the FH-bound GM1 conformation is associated with poor NOE restraints, explaining how it escaped 1 H– 1 H NOE-restrained modeling in the past and highlighting the necessity for ensemble representations of glycan structures.