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  • 1
    Electronic Resource
    Electronic Resource
    The nomenclature of crystallographic symmetry groups (1966)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 21 (1966), S. 1004-1007 
    Details
    ISSN: 0001-5520
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0365110X66004389
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Crystallization and preliminary X-ray study of the edema factor exotoxin adenylyl cyclase domain from Bacillus anthracis in the presence of its activator, calmodulin (2001)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 57 (2001), S. 1881-1884 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Edema factor from Bacillus anthracis is a 92 kDa secreted adenylyl cyclase exotoxin and is activated by the host-resident protein calmodulin. Calmodulin is a ubiquitous intracellular calcium sensor in eukaryotes and activates edema factor nearly 1000-fold upon binding. While calmodulin has many known effectors, including kinases, phosphodiesterases, motor proteins, channels and type 1 adenylyl cyclases, no structures of calmodulin in complex with a functional enzyme have been solved. The crystallization and initial experimental phasing of crystals containing a complex of edema factor adenylyl cyclase domain and calmodulin are reported here. The edema factor–calmodulin complex crystallizes in three different space groups. A native data set in the I222 space group has been collected to 2.7 Å and the self-rotation function solution suggests three edema factor–calmodulin complexes in each asymmetric unit. Initial 4 Å phases were obtained by selenomethionyl MAD in combination with two heavy-atom derivatives. These phases were successfully extended to 2.7 Å using NCS averaging.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444901014937
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  • 3
    Electronic Resource
    Electronic Resource
    High-resolution crystals and preliminary X-ray diffraction studies of a catalytic RNA (1994)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 50 (1994), S. 290-292 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: High-resolution single crystals of a catalytic RNA molecule derived from the sequence of the satellite RNA of tobacco ringspot virus have been obtained. The unit-cell volumes of the RNA crystals vary depending on the crystallization conditions and temperature. The best crystal form, when flash frozen, has space group P1 with unit-cell dimensions a = 53.08, b = 71.81, c = 28.03 Å, α = 98.43, β = 104.32 and γ = 74.54°. This form diffracts to a resolution of 2.4 Å. A heavy-atom derivative search is in progress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444993013071
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  • 4
    Electronic Resource
    Electronic Resource
    Geometrical symbols for all crystallographic symmetry groups up to three dimensions (1967)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 23 (1967), S. 913-933 
    Details
    ISSN: 0001-5520
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0365110X67004050
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  • 5
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    Joint inversion of active and passive seismic data in Central Java (2007)
    Oxford University Press
    Details
    Publication Date: 2007-08-01
    Print ISSN: 0956-540X
    Electronic ISSN: 1365-246X
    Topics: Geosciences
    Published by Oxford University Press on behalf of The Royal Astronomical Society.
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  • 6
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    The B-WICH chromatin-remodelling complex regulates RNA polymerase III transcription by promoting Max-dependent c-Myc binding (2015)
    Oxford University Press
    Details
    Publication Date: 2015-05-20
    Description: The chromatin-remodelling complex B-WICH, comprised of William syndrome transcription factor, the ATPase SNF2h and nuclear myosin, specifically activates RNA polymerase III transcription of the 5S rRNA and 7SL genes. However, the underlying mechanism is unknown. Using high-resolution MN walking we demonstrate here that B-WICH changes the chromatin structure in the vicinity of the 5S rRNA and 7SL RNA genes during RNA polymerase III transcription. The action of B-WICH is required for the binding of the RNA polymerase machinery and the regulatory factors c-Myc at the 5S rRNA and 7SL RNA genes. In addition to the c-Myc binding site at the 5S genes, we have revealed a novel c-Myc and Max binding site in the intergenic spacer of the 5S rDNA. This region also contains a region remodelled by B-WICH. We demonstrate that c-Myc binds to both sites in a Max-dependent way, and thereby activate transcription by acetylating histone H3. The novel binding patterns of c-Myc and Max link transcription of 5S rRNA to the Myc/Max/Mxd network. Since B-WICH acts prior to c-Myc and other factors, we propose a model in which the B-WICH complex is required to maintain an open chromatin structure at these RNA polymerase III genes. This is a prerequisite for the binding of additional regulatory factors.
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
    Published by Oxford University Press
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  • 7
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    OMEGA - OSIRIS Mapping of Emission-line Galaxies in A901/2 - I. Survey description, data analysis, and star formation and AGN activity in the highest density regions (2015)
    Oxford University Press
    Details
    Publication Date: 2015-05-24
    Description: We present an overview of and first results from the OMEGA (OSIRIS Mapping of Emission-line Galaxies in the multicluster system A901/2) survey. The ultimate goal of this project is to study star formation and active galactic nucleus (AGN) activity across a broad range of environments at a single redshift. Using the tuneable-filter mode of the Optical System for Imaging and low-Intermediate-Resolution Integrated Spectroscopy (OSIRIS) instrument on Gran Telescopio Canarias, we target H α and $[\mathrm{N}\,\small {II}]$ emission lines over an ~0.5 0.5 deg 2 region containing the z  ~ 0.167 multicluster system A901/2. In this paper, we describe the design of the survey, the observations and the data analysis techniques developed. We then present early results from two OSIRIS pointings centred on the cores of the A901a and A902 clusters. AGN and star-forming (SF) objects are identified using the $[\mathrm{N}\,\small {II}]\,/\,\mathrm{H}\,\alpha$ versus W H α diagnostic diagram. The AGN hosts are brighter, more massive, and possess earlier type morphologies than SF galaxies. Both populations tend to be located towards the outskirts of the high-density regions we study. The typical H α luminosity of these sources is significantly lower than that of field galaxies at similar redshifts, but greater than that found for A1689, a rich cluster at z  ~ 0.2. The H α luminosities of our objects translate into star formation rates (SFRs) between ~0.02 and 6 M yr –1 . Comparing the relationship between stellar mass and H α-derived SFR with that found in the field indicates a suppression of star formation in the cores of the clusters. These findings agree with previous investigations of this multicluster structure, based on other star formation indicators, and demonstrate the power of tuneable filters for this kind of study.
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
    Published by Oxford University Press
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  • 8
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    Epigenetic control of alternative mRNA processing at the imprinted Herc3/Nap1l5 locus (2012)
    Oxford University Press
    Details
    Publication Date: 2012-10-10
    Description: Alternative polyadenylation increases transcriptome diversity by generating multiple transcript isoforms from a single gene. It is thought that this process can be subject to epigenetic regulation, but few specific examples of this have been reported. We previously showed that the Mcts2 / H13 locus is subject to genomic imprinting and that alternative polyadenylation of H13 transcripts occurs in an allele-specific manner, regulated by epigenetic mechanisms. Here, we demonstrate that allele-specific polyadenylation occurs at another imprinted locus with similar features. Nap1l5 is a retrogene expressed from the paternally inherited allele, is situated within an intron of a ‘host’ gene Herc3 , and overlaps a CpG island that is differentially methylated between the parental alleles. In mouse brain, internal Herc3 polyadenylation sites upstream of Nap1l5 are used on the paternally derived chromosome, from which Nap1l5 is expressed, whereas a downstream site is used more frequently on the maternally derived chromosome. Ablating DNA methylation on the maternal allele at the Nap1l5 promoter increases the use of an internal Herc3 polyadenylation site and alters exon splicing. These changes demonstrate the influence of epigenetic mechanisms in regulating Herc3 alternative mRNA processing. Internal Herc3 polyadenylation correlates with expression levels of Nap1l5 , suggesting a possible role for transcriptional interference. Similar mechanisms may regulate alternative polyadenylation elsewhere in the genome.
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
    Published by Oxford University Press
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  • 9
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    The interaction of Pcf11 and Clp1 is needed for mRNA 3'-end formation and is modulated by amino acids in the ATP-binding site (2012)
    Oxford University Press
    Details
    Publication Date: 2012-02-17
    Description: Polyadenylation of eukaryotic mRNAs contributes to stability, transport and translation, and is catalyzed by a large complex of conserved proteins. The Pcf11 subunit of the yeast CF IA factor functions as a scaffold for the processing machinery during the termination and polyadenylation of transcripts. Its partner, Clp1, is needed for mRNA processing, but its precise molecular role has remained enigmatic. We show that Clp1 interacts with the Cleavage–Polyadenylation Factor (CPF) through its N-terminal and central domains, and thus provides cross-factor connections within the processing complex. Clp1 is known to bind ATP, consistent with the reported RNA kinase activity of human Clp1. However, substitution of conserved amino acids in the ATP-binding site did not affect cell growth, suggesting that the essential function of yeast Clp1 does not involve ATP hydrolysis. Surprisingly, non-viable mutations predicted to displace ATP did not affect ATP binding but disturbed the Clp1–Pcf11 interaction. In support of the importance of this interaction, a mutation in Pcf11 that disrupts the Clp1 contact caused defects in growth, 3'-end processing and transcription termination. These results define Clp1 as a bridge between CF IA and CPF and indicate that the Clp1–Pcf11 interaction is modulated by amino acids in the conserved ATP-binding site of Clp1.
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
    Published by Oxford University Press
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  • 10
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    A search for weak or complex magnetic fields in the B3V star {iota} Herculis (2014)
    Oxford University Press
    Details
    Publication Date: 2014-09-10
    Description: We obtained 128 high signal-to-noise ratio Stokes V spectra of the B3V star Her on five consecutive nights in 2012 with the ESPaDOnS spectropolarimeter at the Canada–France–Hawaii Telescope, with the aim of searching for the presence of weak and/or complex magnetic fields. Least-squares deconvolution (LSD) mean profiles were computed from individual spectra, averaged over individual nights and over the entire run. No Zeeman signatures are detected in any of the profiles. The longitudinal magnetic field in the grand average profile was measured to be –0.24 ± 0.32 G, as compared to –0.22 ± 0.32 G in the null profile. Our observations therefore provide no evidence for the presence of Zeeman signatures analogous to those observed in the A0V star Vega by Lignières et al. We interpret these observations in three ways. First, we compare the LSD profiles with synthetic Stokes V profiles corresponding to organized (dipolar) magnetic fields, for which we find an upper limit of about 8 G on the polar strength of any surface dipole present. Secondly, we compare the grand average profile with calculations corresponding to the random magnetic spot topologies of Kochukhov & Sudnik, inferring that spots, if present, of 2° radius with strengths of 2–4 G and a filling factor of 50 per cent should have been detected in our data. Finally, we compare the observations with synthetic V profiles corresponding to the surface magnetic maps of Vega (Petit et al.) computed for the spectral characteristics of Her. We conclude that while it is unlikely we would have detected a magnetic field identical to Vega's, we would have likely detected one with a peak strength of about 30 G, i.e. approximately four times as strong as that of Vega.
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
    Published by Oxford University Press
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