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  • Springer  (500)
  • Oxford University Press  (108)
  • 2000-2004  (178)
  • 1990-1994  (430)
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  • 1
    Electronic Resource
    Electronic Resource
    Metal substitution in transferrins: specific binding of cerium(IV) revealed by the crystal structure of cerium-substituted human lactoferrin (2000)
    Springer
    JBIC 5 (2000), S. 692-698 
    Details
    ISSN: 1432-1327
    Keywords: Metal substitution Cerium binding Lactoferrin Crystal structure Transferrin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. Proteins of the transferrin family play a key role in iron homeostasis through their extremely strong binding of iron, as Fe3+. They are nevertheless able to bind a surprisingly wide variety of other metal ions. To investigate how metal ions of different size, charge and coordination characteristics are accommodated, we have determined the crystal structure of human lactoferrin (Lf) complexed with Ce4+. The structure, refined at 2.2 Å resolution (R=20.2%, R free=25.7%) shows that the two Ce4+ ions occupy essentially the same positions as do Fe3+, and that the overall protein structure is unchanged; the same closed structure is formed for Ce2Lf as for Fe2Lf. The larger metal ion is accommodated by small shifts in the protein ligands, made possible by the presence of water molecules adjacent to each binding site. The two Ce4+ sites are equally occupied, indicating that the known difference in the pH-dependent release of Ce4+ arises from a specific protonation event, possibly of the His ligand in one of the binding sites. Comparing the effects of binding Ce4+ with those for the binding of other metal ions, we conclude that the ability of transferrins to accommodate metal ions other than Fe3+ depends on an interplay of charge, size, coordination and geometrical preferences of the bound metal ion. However, it is the ability to accept the six-coordinate, approximately octahedral, site provided by the protein that is of greatest importance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007750000157
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  • 2
    Electronic Resource
    Electronic Resource
    Technical Opportunities for International Collaborations by the U.S. Fusion Program (2000)
    Springer
    Journal of fusion energy 19 (2000), S. 65-79 
    Details
    ISSN: 1572-9591
    Keywords: Fusion energy ; international collaboration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract This report was prepared by a Working Group at the request of the U.S. Department of Energy, Office of Fusion Energy Sciences in 1997. The report addresses technical opportunities for mutually beneficial collaboration between the United States and other international fusion research programs. A number of outstanding opportunities are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1012281826821
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  • 3
    Electronic Resource
    Electronic Resource
    Ecological and evolutionary implications of digestive processes: Bird preferences and the sugar constituents of floral nectar and fruit pulp (1992)
    Springer
    Cellular and molecular life sciences 48 (1992), S. 544-551 
    Details
    ISSN: 1420-9071
    Keywords: Bird feeding preferences ; digestion ; intestinal enzymes ; sucrase ; fruit ; nectar
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Plants pollinated and dispersed by different groups of birds offer different kinds of sugars in nectar and fruit pulp. The preferences and physiological traits of avian pollinators and seed dispersers are broadly correlated with the sugar composition of the nectar and fruit that they feed on and appear to have influenced the evolution of the sugar composition of the rewards that plants offer. Hummingbirds prefer sucrose whereas many nectar- and fruit-eating passerines prefer glucose and fructose. Preference for hexoses in passerines seems to be associated with poor sucrose assimilation resulting from two physiological mechanisms: lack of intestinal sucrase activity and fast passage rates. Sucrase activity absence appears to be restricted to a single phylogenetic group (the sturnid-muscicapid lineage). Fast passage rates seem to be characteristic of many small frugivores and to hinder the assimilation of complex nutrients that require hydrolysis before absorption. Hummingbirds have extremely specialized digestive traits that allow them to assimilate sucrose at high rates and with extremely high efficiency. These specialized digestive traits appear not to be present in many nectar-feeding passerines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01920237
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  • 4
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    Ecological and evolutionary implications of digestive processes: Bird preferences and the sugar constituents of floral nectar and fruit pulp (1992)
    Springer
    Details
    Publication Date: 1992-06-01
    Print ISSN: 0014-4754
    Topics: Biology , Medicine
    Published by Springer
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    PAPER CURRENT
  • 5
    Electronic Resource
    Electronic Resource
    Membrane-associated echinocandin B deacylase of Actinoplanes utahensis: purification, characterization, heterologous cloning and enzymatic deacylation reaction (2000)
    Springer
    Journal of industrial microbiology and biotechnology 24 (2000), S. 173-180 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: echinocandin B deacylase; substrate specificity; evolution/technology; antifungal agent
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aspergillus nidulans produces echinocandin B, a neutral lipopeptide. A deacylase from Actinoplanes utahensis catalyzes cleavage of the linoleoyl group from echinocandin B, a key step in generating a potential antifungal agent. Virtually all (99.8%) deacylase activity was cell-associated. The deacylase was salt-solubilized, heat-treated and purified to apparent homogeneity by a 3-step chromatographic procedure. The enzyme was a heterodimer consisting of 63- and 18-to-20-kDa subunit, optimally active at pH 6.0, and at 60°C with salt. The K m of the deacylase for echinocandin B was 50 μM and its V max was 14.6 μmol cyclic hexapeptide min−1 mg−1protein. The substrate specificity of the enzyme was broad with respect to both acyl and cyclic peptide analogues of echinocandin B. The two deacylase subunit genes were cloned and over-expressed in Streptomyces lividans. The recombinant deacylase was purified from the culture filtrate to apparent homogeneity by a 1-step chromatographic procedure. Using the recombinant deacylase, an enzymatic deacylation of immobilized echinocandin B resulted in the generation of cyclic hexapeptide at gram-level. Journal of Industrial Microbiology & Biotechnology (2000) 24, 173–180.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900796
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  • 6
    Electronic Resource
    Electronic Resource
    Sums of cubes of square-free numbers (1991)
    Springer
    Monatshefte für Mathematik 111 (1991), S. 1-21 
    Details
    ISSN: 1436-5081
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Almost all positive integers may be represented as the sum of four cubes of positive square-free integers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01299273
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  • 7
    Electronic Resource
    Electronic Resource
    Sums of cubes of square-free numbers II (1991)
    Springer
    Monatshefte für Mathematik 112 (1991), S. 177-207 
    Details
    ISSN: 1436-5081
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Letr s (n) denote the number of representations ofn as the sum ofs cubes of square-free numbers. We prove an asymptotic formula forr s (n) and a strong lower bound forr 7 (n).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01297338
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  • 8
    Electronic Resource
    Electronic Resource
    A Method for Architecting Product Platforms (2000)
    Springer
    Research in engineering design 12 (2000), S. 61-72 
    Details
    ISSN: 1435-6066
    Keywords: Key words:Product families – Product platforms, Product portfolio architecture.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract: Consider a group of products sharing common parts and assemblies. The products in question we call a product family, and the common elements, the platform. In this paper, we present a method for designing product platforms and the derived family that takes into consideration both the technical performance requirements as well as the cost of the product family. The design of a platform-based product family is formulated as a general optimization problem in which the advantages of designing a common platform must be balanced against the constraints of the individual product variants and constraints of the family as a whole. This optimization approach forms the basis for a practical implementation as an interactive, team-based negotiation model for designing a family of interplanetary spacecraft based on a common platform. The approach is used to consider and specify different subsystems that could be made common to all the missions. It is also used to evaluate the impact of those platform design decisions on the performance of the product family, and thus be able to select from among feasible platform designs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001630050024
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  • 9
    Electronic Resource
    Electronic Resource
    Unraveling the intraspecific phylogeography of snotsCalidris canutus: a progress report on the search for genetic markers (1994)
    Springer
    Journal of ornithology 135 (1994), S. 599-608 
    Details
    ISSN: 1439-0361
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mitochondrial DNA control region sequences of 25 Knots sampled from 10 populations and possibly four subspecies (canutus, islandica, rogersi, rufa) were obtained by PCR and direct sequencing. Only 7 haplotypes were found worldwide, all closely related to one another and differing by 1–3 substitutions. Knots have most probably expanded to their current population size from a refugial population that was severely bottlenecked late in the Pleistocene. Preliminary results from RAPDs are consistent with this prediction, in that Knots from North America appear to be genetically distinct from Knots elsewhere.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01649854
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  • 10
    Electronic Resource
    Electronic Resource
    Antiproton-proton annihilation at rest into two-body final states (1993)
    Springer
    The European physical journal 58 (1993), S. 175-189 
    Details
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We report measurements of branching ratios for production of a series of two meson final states in $$\bar p$$ p annihilations at rest in liquid hydrogen. We find: $$\begin{gathered} BR(\bar pp \to \pi ^ + \pi ^ - ) = (3.07 \pm 0.13) \cdot 10^{ - 3} \hfill \\ BR(\bar pp \to K^ + K^ - ) = (0.99 \pm 0.05) \cdot 10^{ - 3} \hfill \\ BR(\bar pp \to \pi ^0 \pi ^0 ) = (6.93 \pm 0.43) \cdot 10^{ - 4} \hfill \\ BR(\bar pp \to \pi ^0 \eta ) = (2.12 \pm 0.12) \cdot 10^{ - 4} \hfill \\ BR(\bar pp \to \pi ^0 \omega ) = (5.73 \pm 0.47) \cdot 10^{ - 3} \hfill \\ BR(\bar pp \to \pi ^0 \eta ') = (1.23 \pm 0.13) \cdot 10^{ - 4} \hfill \\ BR(\bar pp \to \eta \eta ) = (1.64 \pm 0.10) \cdot 10^{ - 4} \hfill \\ BR(\bar pp \to \eta \omega ) = (1.51 \pm 0.12) \cdot 10^{ - 2} \hfill \\ BR(\bar pp \to \eta \eta ') = (2.16 \pm 0.25) \cdot 10^{ - 4} \hfill \\ BR(\bar pp \to \omega \omega ) = (3.32 \pm 0.34) \cdot 10^{ - 2} \hfill \\ BR(\bar pp \to \omega \eta ') = (0.78 \pm 0.08) \cdot 10^{ - 2} \hfill \\ \end{gathered}$$ These are the first measurements of the channels ηη′ and ωη′ and in almost all the other channels are more precise than previous results. We also obtain, in a more precise fashion, the following ratios of branching ratios:K + K −/π+π−=0.323±0.013, π0η′/π0η=0.548±0.056, ηη′/ηη=0.31±0.15, ωη′/ωη=0.515±0.040, π0η/π0π0=0.303±0.010, ηη/π0π0=0.232±0.011 and π0ω/ηω=0.377±0.12. The measurements are made for different η and η′ decays, and we thus obtain Γη→3π 0/Γη→γγ=0.841±0.034, and $$\Gamma _{\eta ' \to \gamma \gamma } /\Gamma _{\eta ' \to \pi ^0 \pi ^0 \eta } = 0.091 \pm 0.009$$ .
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01560336
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