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  • American Geophysical Union  (4)
  • Oxford University Press  (4)
  • Paleontological Society
  • American Meteorological Society (AMS)
  • 2010-2014  (8)
  • 1990-1994
  • 2012  (6)
  • 2011  (2)
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  • 2010-2014  (8)
  • 1990-1994
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  • 1
    Publikationsdatum: 2012-04-05
    Beschreibung: Immune responses induced by glycans upon infection with Schistosoma mansoni may be mediated by either schistosomal glycoproteins or glycosphingolipids. In this study, we have elucidated the structural features of both carbohydrate moieties and respective ceramide units of complex glycosphingolipids from adult S. mansoni . Obtained data revealed a vast structural heterogeneity due to manifold combinations of different oligosaccharides and ceramide entities. Observed carbohydrate moieties included Lewis X (Le X ; Gal(β1-4)[Fuc(α1-3)]GlcNAc) as well as, in part, multiply fucosylated LacdiNAc (LDN; GalNAc(β1-4)GlcNAc) carbohydrate epitopes. Corresponding lipid portions comprised predominantly C18-sphingosine as well as C18- and C20-phytosphingosine derivatives. Intriguingly, glycosphingolipids carrying an Le X epitope contained predominantly C18-sphingosine, whereas LDN-based species exhibited mostly phytosphingosine derivatives, in addition to C18-sphingosine, indicating that the two classes of glycosphingolipids might be synthesized via different biosynthetic routes. Compared with literature data, adult worm glycosphingolipids with Le X epitopes revealed clear structural differences in comparison to corresponding cercarial species which have been shown to exhibit mainly sphinganine bases with 18–21 carbon atoms. Therefore, it may be hypothesized that the divergent structural features of the respective ceramide moieties are responsible for the published observation that only adult worm, but not cercarial glycosphingolipids are able to induce dendritic cell activation skewing the T-cell response toward a Th1 profile.
    Print ISSN: 0959-6658
    Digitale ISSN: 1460-2423
    Thema: Biologie , Medizin
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Publikationsdatum: 2012-06-28
    Beschreibung: The Drosophila Suppressor of Hairy-wing [Su(Hw)] protein is a globally expressed, multi-zinc finger (ZnF) DNA-binding protein. Su(Hw) forms a classic insulator when bound to the gypsy retrotransposon and is essential for female germline development. These functions are genetically separable, as exemplified by Su(Hw) f that carries a defective ZnF10, causing a loss of insulator but not germline function. Here, we completed the first genome-wide analysis of Su(Hw)-binding sites (SBSs) in the ovary, showing that tissue-specific binding is not responsible for the restricted developmental requirements for Su(Hw). Mapping of ovary Su(Hw) f SBSs revealed that female fertility requires binding to only one third of the wild-type sites. We demonstrate that Su(Hw) f retention correlates with binding site affinity and partnership with Modifier of (mdg4) 67.2 protein. Finally, we identify clusters of co-regulated ovary genes flanked by Su(Hw) f bound sites and show that loss of Su(Hw) has limited effects on transcription of these genes. These data imply that the fertility function of Su(Hw) may not depend upon the demarcation of transcriptional domains. Our studies establish a framework for understanding the germline Su(Hw) function and provide insights into how chromatin occupancy is achieved by multi-ZnF proteins, the most common transcription factor class in metazoans.
    Print ISSN: 0305-1048
    Digitale ISSN: 1362-4962
    Thema: Biologie
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Publikationsdatum: 2012-01-11
    Print ISSN: 0959-6658
    Digitale ISSN: 1460-2423
    Thema: Biologie , Medizin
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Publikationsdatum: 2012-02-09
    Print ISSN: 0305-1048
    Digitale ISSN: 1362-4962
    Thema: Biologie
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Publikationsdatum: 2012-10-01
    Print ISSN: 0148-0227
    Digitale ISSN: 2156-2202
    Thema: Geologie und Paläontologie
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Publikationsdatum: 2011-02-15
    Print ISSN: 0096-3941
    Digitale ISSN: 2324-9250
    Thema: Geologie und Paläontologie
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Publikationsdatum: 2022-05-25
    Beschreibung: Author Posting. © American Geophysical Union, 2010. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 115 (2010): C12024, doi:10.1029/2009JC006061.
    Beschreibung: Turbulent mixing of salt is examined in a shallow salt wedge estuary with strong fluvial and tidal forcing. A numerical model of the Merrimack River estuary is used to quantify turbulent stress, shear production, and buoyancy flux. Little mixing occurs during flood tides despite strong velocities because bottom boundary layer turbulence is dislocated from stratification elevated in the water column. During ebbs, bottom salinity fronts form at a series of bathymetric transitions. At the fronts, near-bottom velocity and shear stress are low, but shear, stress, and buoyancy flux are elevated at the pycnocline. Internal shear layers provide the dominant source of mixing during the early ebb. Later in the ebb, the pycnocline broadens and moves down such that boundary layer turbulence dominates mixing. Mixing occurs primarily during ebbs, with internal shear mixing accounting for about 50% of the total buoyancy flux. Both the relative contribution of internal shear mixing and the mixing efficiency increase with discharge, with bulk mixing efficiencies between 0.02 and 0.07. Buoyancy fluxes in the estuary increase with discharge up to about 400 m3 s−1 above which a majority of the mixing occurs offshore. Observed buoyancy fluxes were more consistent with the k-ɛ turbulence closure than the Mellor-Yamada closure, and more total mixing occurred in the estuary with k-ɛ. Calculated buoyancy fluxes were sensitive to horizontal grid resolution, as a lower resolution grid yielded less integrated buoyancy flux in the estuary and exported lower salinity water but likely had greater numerical mixing.
    Beschreibung: This research was funded by National Science Foundation Grant OCE‐0452054. Ralston also received support from The Penzance Endowed Fund in Support of Assistant Scientists and The John F. and Dorothy H. Magee Fund in Support of Scientific Staff at Woods Hole Oceanographic Institution.
    Schlagwort(e): Mixing ; Turbulence ; Salt wedge estuary
    Repository-Name: Woods Hole Open Access Server
    Materialart: Article
    Format: application/pdf
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Publikationsdatum: 2022-05-25
    Beschreibung: Author Posting. © American Geophysical Union, 2012. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 117 (2012): C10013, doi:10.1029/2012JC008124.
    Beschreibung: Analyses of field observations and numerical model results have identified that sediment transport in the Hudson River estuary is laterally segregated between channel and shoals, features frontal trapping at multiple locations along the estuary, and varies significantly over the spring-neap tidal cycle. Lateral gradients in depth, and therefore baroclinic pressure gradient and stratification, control the lateral distribution of sediment transport. Within the saline estuary, sediment fluxes are strongly landward in the channel and seaward on the shoals. At multiple locations, bottom salinity fronts form at bathymetric transitions in width or depth. Sediment convergences near the fronts create local maxima in suspended-sediment concentration and deposition, providing a general mechanism for creation of secondary estuarine turbidity maxima at bathymetric transitions. The lateral bathymetry also affects the spring-neap cycle of sediment suspension and deposition. In regions with broad, shallow shoals, the shoals are erosional and the channel is depositional during neap tides, with the opposite pattern during spring tides. Narrower, deeper shoals are depositional during neaps and erosional during springs. In each case, the lateral transfer is from regions of higher to lower bed stress, and depends on the elevation of the pycnocline relative to the bed. Collectively, the results indicate that lateral and along-channel gradients in bathymetry and thus stratification, bed stress, and sediment flux lead to an unsteady, heterogeneous distribution of sediment transport and trapping along the estuary rather than trapping solely at a turbidity maximum at the limit of the salinity intrusion.
    Beschreibung: This research was funded by a grant from the Hudson River Foundation (#002/07A). D.R. was partially supported by the Office of Naval Research (N00014-08-1-0846).
    Beschreibung: 2013-04-17
    Schlagwort(e): Estuarine turbidity maximum ; Lateral sediment distribution ; Salinity fronts ; Sediment flux ; Sediment trapping ; Stratification
    Repository-Name: Woods Hole Open Access Server
    Materialart: Article
    Format: application/pdf
    Standort Signatur Erwartet Verfügbarkeit
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