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  • Nature Publishing Group  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 219 (1968), S. 172-173 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Lymphocytes were prepared from human venous blood as previously described8. The erythrocytes were allowed to settle and the phagocytic cells were removed from the supernatant by passing it through a nylon fibre column. The cells which eluted from the column (more than 98 per cent lymphocytes) were ...
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 215 (1967), S. 77-78 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] DL-l-14C-leucine (32 mc./mmole) was used in these experiments. Solid PHA was reconstituted in tissue culture medium TC199, and used at a concentration of 0.015 ml./ml. of lymphocyte suspension. Lymphocytes were prepared from human blood by sedimentation with gelatine8. The supernatant was diluted ...
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 215 (1967), S. 737-738 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Lymphocyte cultures were prepared and incubated as previously described4. After incubation of cultures for the time required, medium containing either tritiated thymidine (3.3 μc./ml.) or 14C leucine (1 μmc./ml.) was added and isotope incorporation was determined after incubation of the ...
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