ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Munksgaard International Publishers  (1)
Collection
Publisher
Years
  • 1
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 102 (1998), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Previously, B12D and B22E have been characterized as Barley aleurone and embryo (Balem) transcripts, expressed during seed maturation and embryo germination. The open reading frame of B12D cDNAs encodes a protein of unknown function highly conserved in mono- and dicotyledonous species, while B22E encodes a metallothionein-like protein. Several slightly different B22E transcripts have earlier been identified. Our objective was to investigate the number of B12D genes, and B12D and B22E expression patterns in mature aleurone. Genomic Southern hybridization and primer extension experiments suggest the presence of a B12D gene family in barley with at least 8 or 9 members. B12D transcripts can also be identified in the starchy endosperm, and a primer extension analysis indicates that some of these genes are expressed in the starchy endosperm only. A number of genes appear to be transcribed in all tissues investigated; starchy endosperm, pericarp, immature and mature embryos and aleurone, and mature aleurone incubated with GA3. One B12D gene, HvB12Dg1, was isolated and shown by particle bombardment with a promoter-GUS construct to be transcriptionally active. The HvB12Dg1 promoter contains elements similar to those of the gibberellic acid response complex (GARC). B12D transcripts are found in the aleurone of imbibed embryoless grains, while B22E transcripts are barely detectable. However, both transcripts are up-regulated by the presence of the germinating embryo. For B22E this effect is not mimicked by applying GA3 exogenously to imbibed embryoless grains, while the B12D transcript level increases 2- to 3-fold, at most. On the other hand, ABA can suppress B12D expression. Our investigations indicate that gibberellic acid may not be directly involved in the up-regulation of all transcripts induced in the aleurone during germination.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...