ALBERT

Description: The intensity and frequency of droughts in Poyang Lake Basin have been increasing due to global warming. To properly manage water resources and mitigate drought disasters, it is important to understand the long-term characteristics of drought and its possible link with large-scale climate indices. Based on the monthly meteorological data of 41 meteorological stations in Poyang Lake Basin from 1958 to 2017, the spatiotemporal variations of drought were investigated using the standardized precipitation evapotranspiration index (SPEI). Ensemble empirical mode decomposition (EEMD) methods and the modified Mann–Kendall (MMK) trend test were used to explore the spatiotemporal characteristics and trends of drought. Furthermore, to reveal possible links between drought variations and large-scale climate indices in Poyang Lake Basin, the relationships between SPEI and large-scale climate indices, such as North Atlantic Oscillation (NAO), El Niño–Southern Oscillation (ENSO), Arctic Oscillation (AO), Indian Ocean Dipole (IOD) and Pacific Decadal Oscillation (PDO) were examined using cross-wavelet transform. The results showed that the SPEI in Poyang Lake Basin exhibited relatively stable quasi-periodic oscillation, with approximate quasi-3-year and quasi-6-year periods at the inter-annual scale and quasi-15-year and quasi-30-year periods at the inter-decadal scale from 1958 to 2017. Moreover, the Poyang Lake Basin experienced an insignificantly wetter trend as a whole at the annual and seasonal scales during the period of 1958–2017, except for spring, which had a drought trend. The special characteristics of the trend variations were markedly different in the basin. The areas in which drought was most likely to occur were mainly located in the Poyang Lake region, northwest and south of the basin, respectively. Furthermore, relationships between the drought and six climate indices showed that the drought exhibited a significant temporal correlation with five climate indices at restricted intervals, except for IOD. The dominant influences of the large-scale climate indices on the drought evolutions shifted in the Poyang Lake Basin during 1958–2017, from the NAO, Niño 3.4, and the Southern Oscillation Index (SOI) before the late 1960s and early 1970s, to the AO and PDO during the 1980s, then to the NAO, AO and SOI after the early 2000s. The NAO, AO and SOI exerted a significant influence on the drought events in the basin. The results of this study will benefit regional water resource management, agriculture production, and ecosystem protection in the Poyang Lake Basin.

Description: Vitellogenin receptors (VgRs) play critical roles in egg formation by transporting vitellogenin (Vg) into oocytes in insects. Although the function of VgR in insects is well studied, the transcriptional regulation of this gene is still unclear. Here, we cloned the promoter of the VgR gene from Bombyx mori (BmVgR), and predicted many POU cis-response elements (CREs) in its promoter. Electrophoretic mobility shift and chromatin immunoprecipitation assays showed that the POU transcription factor POU-M2 bound directly to the CREs of the promoter. Overexpression of POU-M2 in an ovarian cell line (BmNs) enhanced BmVgR transcription and promoter activity detected by quantitative reverse transcription PCR and luciferase reporter assays. Analyses of expression patterns indicated that POU-M2 was expressed in ovary at day two of wandering stage initially, followed by BmVgR. RNA interference of POU-M2 significantly reduced the transcription of BmVgR in ovary and egg-laying rate. Our results suggest a novel function for the POU factor in silkworm oogenesis by its involvement in BmVgR regulation and expands the understanding of POU factors in insect VgR expression.

Description: While the use of topical drops for the delivery of drugs to the anterior of the eye is well accepted, it is far from efficient with as little as 5% of the drug instilled on the eye actually reaching the target tissue. The ability to prolong the residence time on the eye is desirable. Based on the acceptability of 2-hydroxyethyl methacrylate based polymers in contact lens applications, the current work focuses on the development of a poly(2-hydroxyethyl methacrylate (HEMA)) nanoparticle system. The particles were modified to allow for degradation and to permit mucoadhesion. Size and morphological analysis of the final polymer products showed that nano-sized, spherical particles were produced. FTIR spectra demonstrated that the nanoparticles comprised poly(HEMA) and that 3-(acrylamido)phenylboronic acid (3AAPBA), as a mucoadhesive, was successfully incorporated. Degradation of nanoparticles containing N,N′-bis(acryloyl)cystamine (BAC) after incubation with DL-dithiothreitol (DTT) was confirmed by a decrease in turbidity and through transmission electron microscopy (TEM). Nanoparticle mucoadhesion was shown through an in-vitro zeta potential analysis.

Description: Wooden structures in China’s ancient buildings hold highly historical and cultural values. There is an urgent need to repair and replace the damaged wooden structures after hundreds and thousands of years of exposure to weather. Unfortunately, to date there is still a lack of insightful understanding on how the chemical structure, composition, and micro-morphology evolve over the long-term natural aging before artificial ancient timbers can be developed. This work aims to systematically examine the outer surface, middle layer, and inner surface of the same piece of Chinese fir (Cunninghamia lanceolate) collected from an ancient Chinese building. Based on qualitative and quantitative analysis, both cellulose and hemicellulose in aged woods are found to experience significant degrees of degradation. The crystalline regions of cellulose are also determined to undergo moderate degradation as compared to the control fresh wood. In comparison, the lignin basically remains unchanged and its content in the inner layer slightly increases, as evidenced by more free phenol groups determined. Relative to the outer and inner layer, the middle layer of the ancient wood shows the lowest degree of degradation close to that of the fresh wood. This work offers guidelines for fabricating artificial ancient woods to repair the destroyed ones in China’s ancient architectures.

Description: The household sector, which plays a critical role in emission reduction, is a main source of greenhouse gas (GHG) emissions. Although numerous academic journals have published papers on household CO2 emissions (HCEs), great challenges remain in research on assessments, determinants, and further research prospects. This work reviews and projects HCEs using a bibliometric analysis and a systematic review based on the data from the Web of Science (WOS) platform from 1991 to 2020. Over the last 30 years, there has been a rapid and active trend of research on HCEs. We find that (1) the scale of the bibliometric analysis shows that research on HCEs is interdisciplinary and must consider overall cognition of the environment, the economy, society, and technology. It also needs to strengthen cooperation between different countries/territories to emphasize the quality and influence of papers on HCEs. (2) A review of previous literature shows that research on HCEs mainly focuses on the research object, mainstream assessments, and influencing factors. The following six main aspects impact HCEs: demographic, income, social, technological, policy, and natural factors. (3) The research discussion suggests that more micro-level research needs to be conducted, such as research on the city level and the individual level, which is important for sustainable development and low consumption. A comparative analysis of the differences in HCEs is a future research direction. Additionally, localized carbon emission reduction measures need to be implemented.

Description: The Chinese government launched policies supporting biodiesel production and innovation to meet sustainable energy demands under rapid economic and social development. This study systematically investigates the dynamic transitions of biodiesel policies in China regarding policy contents and policy networks. We performed a content analysis in this study and used NVivo12 software to support the analysis process. The results highlighted some issues. First, the Chinese government supported the development of biodiesel from multiple policy objectives and adopted various policy instruments, eventually transforming biodiesel in China from the first- to second-generation. Second, the State Council was the main institution to promote biodiesel policies, and the National Development and Reform Commission under the State Council burdened most of the responsibilities of policy implementation. Most of the policies were issued at a lower level of administration rather than a higher level of decision-making. Biodiesel policies in China were gradually detailed, and they constantly established a system of technology and product innovation.

Description: MYB transcription factors (TFs) are one of the largest TF families in plants to regulate numerous biological processes. However, our knowledge of the MYB family in Physcomitrella patens is limited. We identified 116 MYB genes in the P. patens genome, which were classified into the R2R3-MYB, R1R2R3-MYB, 4R-MYB, and MYB-related subfamilies. Most R2R3 genes contain 3 exons and 2 introns, whereas R1R2R3 MYB genes contain 10 exons and 9 introns. N3R-MYB (novel 3RMYB) and NR-MYBs (novel RMYBs) with complicated gene structures appear to be novel MYB proteins. In addition, we found that the diversity of the MYB domain was mainly contributed by domain shuffling and gene duplication. RNA-seq analysis suggested that MYBs exhibited differential expression to heat and might play important roles in heat stress responses, whereas CCA1-like MYB genes might confer greater flexibility to the circadian clock. Some R2R3-MYB and CCA1-like MYB genes are preferentially expressed in the archegonium and during the transition from the chloronema to caulonema stage, suggesting their roles in development. Compared with that of algae, the numbers of MYBs have significantly increased, thus our study lays the foundation for further exploring the potential roles of MYBs in the transition from aquatic to terrestrial environments.

Description: Manganese, as an essential trace element, participates in many physiological reactions by regulating Mn associated enzymes. Magnaporthe oryzae is a serious pathogen and causes destructive losses for rice production. We identified a cytochrome P450 gene, MoMCP1, involving the alleviation of manganese toxin and pathogenicity. To identify the underlying mechanisms, transcriptomics were performed. The results indicated that many pathogenicity related genes were regulated, especially hydrophobin related genes in ∆Momcp1. Furthermore, the Mn2+ toxicity decreased the expressions of genes involved in the oxidative phosphorylation and energy production, and increased the reactive oxygen species (ROS) levels, which might impair the functions of mitochondrion and vacuole, compromising the pathogenicity and development in ∆Momcp1. Additionally, our results provided further information about Mn associated the gene network for Mn metabolism in cells.

Description: There is a strong correlation between the geometry of the weld pool surface and the degree of penetration in arc welding. To measure the geometry of the weld pool surface robustly, many structured light laser line based monitoring systems have been proposed in recent years. The geometry of the specular weld pool could be computed from the reflected laser lines based on different principles. The prerequisite of accurate computation of the weld pool surface is to segment the reflected laser lines robustly and efficiently. To find the most effective segmentation solutions for the images captured with different welding parameters, different image processing algorithms are combined to form eight approaches and these approaches are compared both qualitatively and quantitatively in this paper. In particular, the gradient detection filter, the difference method and the GLCM (grey level co-occurrence matrix) are used to remove the uneven background. The spline fitting enhancement method is used to remove the fuzziness. The slope difference distribution-based threshold selection method is used to segment the laser lines from the background. Both qualitative and quantitative experiments are conducted to evaluate the accuracy and the efficiency of the proposed approaches extensively.