ALBERT

Beschreibung: This study investigates the possible involvement of maize aquaporins which are regulated by arbuscular mycorrhizae (AM) in the transport in planta of ammonium and/or urea under well-watered and drought stress conditions. The study also aims to better understand the implication of the AM symbiosis in the uptake of urea and ammonium and its effect on plant physiology and performance under drought stress conditions. AM and non-AM maize plants were cultivated under three levels of urea or ammonium fertilization (0, 3 µM or 10 mM) and subjected or not to drought stress. Plant aquaporins and physiological responses to these treatments were analyzed. AM increased plant biomass in absence of N fertilization or under low urea/ ammonium fertilization, but no effect of the AM symbiosis was observed under high N supply. This effect was associated with reduced oxidative damage to lipids and increased N accumulation in plant tissues. High N fertilization with either ammonium or urea enhanced net photosynthesis (AN) and stomatal conductance (gs) in plants maintained under well-watered conditions, but 14 days after drought stress imposition these parameters declined in AM plants fertilized with high N doses. The aquaporin ZmTIP1;1 was up-regulated by both urea and ammonium and could be transporting these two N forms in planta. The differential regulation of ZmTIP4;1 and ZmPIP2;4 with urea fertilization and of ZmPIP2;4 with NH4+ supply suggests that these two aquaporins may also play a role in N mobilization in planta. At the same time, these aquaporins were also differentially regulated by the AM symbiosis, suggesting a possible role in the AM-mediated plant N homeostasis that deserves future studies.

Beschreibung: Boron (B) is an essential micronutrient for higher plants, having structural roles in primary cell walls, but also other functions in cell division, membrane integrity, pollen germination or metabolism. Both high and low B levels negatively impact crop performance. Thus, plants need to maintain B concentration in their tissues within a narrow range by regulating transport processes. Both active transport and protein-facilitated diffusion through aquaporins have been demonstrated. This study aimed at elucidating the possible involvement of some plant aquaporins, which can potentially transport B and are regulated by the arbuscular mycorrhizal (AM) symbiosis in the plant B homeostasis. Thus, AM and non-AM plants were cultivated under 0, 25 or 100 μM B in the growing medium and subjected or not subjected to drought stress. The accumulation of B in plant tissues and the regulation of plant aquaporins and other B transporters were analyzed. The benefits of AM inoculation on plant growth (especially under drought stress) were similar under the three B concentrations assayed. The tissue B accumulation increased with B availability in the growing medium, especially under drought stress conditions. Several maize aquaporins were regulated under low or high B concentrations, mainly in non-AM plants. However, the general down-regulation of aquaporins and B transporters in AM plants suggests that, when the mycorrhizal fungus is present, other mechanisms contribute to B homeostasis, probably related to the enhancement of water transport, which would concomitantly increase the passive transport of this micronutrient.