Publication Date:
2023-02-08
Description:
An endophytic actinomycete, strain 3MP-10 T, isolated from the root of Mimosa pudica was taxonomically studied based upon polyphasic approaches. This strain formed spiral spore chains on aerial mycelia. ll -Diaminopimelic acid, glucose and ribose were found in the whole-cell hydrolysates. It belonged to the genus Streptomyces and was closely related to Streptomyces zhaozhouensis DSM 42101 T (98.9 %) and Streptomyces sedi JCM 16909 T (98.6 %) based on 16S rRNA gene sequence analysis results. The major menaquinones were MK-10(H 8 ), MK-10(H 6 ) and MK-9(H 8 ). The predominant cellular fatty acids were iso - C 16 : 0 , anteiso-C 15 : 0 and anteiso - C 17 : 0 . The detected phospholipids were diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phosphatidylethanolamine and phosphatidylglycerol. Strain 3MP-10 T had a genome size of 7.2 Mb with a genome G+C content of 73.4 mol%. Results of in silico genome-based similarity analysis revealed ANIb values of 84.94 and 84.77 %, ANIm values of 88.01 and 87.92 %, and dDDH values of 29.9 and 29.6 % when compared with S. zhaozhouensis DSM 42101 T and S. sedi JCM 16909 T , respectively. Based on the polyphasic approach, digital DNA–DNA relatedness and average nucleotide identity, we propose that the novel actinomycete represents a novel species, Streptomyces mimosae , with type strain 3MP-10 T (=JCM 33328 T =TISTR 2646 T ).
Type:
Article
,
PeerReviewed
Format:
text
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