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  • 1
    Publication Date: 2015-12-10
    Description: This study aimed to explore the effects of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) on apoptosis and cell cycle in a zebrafish (Danio rerio) liver cell line (ZFL). Treatment groups included a control group, PFOA-IC50, PFOA-IC80, PFOS-IC50 and PFOS-IC80 groups. IC50 and IC80 concentrations were identified by cellular modeling and MTT assays. mRNA levels of p53, Bcl-2, Bax, Caspase-3 and NF-κB p65 were detected by qPCR. Cell apoptosis and cell cycle were detected by flow cytometry and the protein levels of p53, Bcl-2, Bax, Caspase-3 and NF-κB p65 were determined by western blotting. Both PFOA and PFOS inhibited the growth of zebrafish liver cells, and the inhibition rate of PFOS was higher than that of PFOA. Bcl-2 expression levels in the four groups were significantly higher than the control group and Bcl-2 increased significantly in the PFOA-IC80 group. However, the expression levels of Bax in the four treatment groups were higher than the control group. The percentage of cell apoptosis increased significantly with the treatment of PFOA and PFOS (p 〈 0.05). Cell cycle and cell proliferation were blocked in both the PFOA-IC80 and PFOS-IC80 groups, indicating that PFOA-IC80 and PFOS-IC50 enhanced apoptosis in ZFL cells.
    Print ISSN: 1661-7827
    Electronic ISSN: 1660-4601
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Published by MDPI Publishing
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  • 2
    Publication Date: 2016-12-07
    Description: Nature reserves are established to protect ecosystems and rare flora and fauna. However, with the rapid development of the social economy, many nature reserves are facing enormous pressures from human activities. The assessment of the sustainability of nature reserves is a fundamental task for the planning and management of such areas. In this study, the sustainability of China’s 319 national nature reserves (NRRs) was evaluated based on an ecological footprint (EF) method. The results indicated that the per capita ecological footprints of all national nature reserves increased 85.86% from 2000 to 2010. Meanwhile, the per capita biocapacity (BC) of all national nature reserves increased slightly, with a rate of increase of 1.79%. The ‘traffic light’ method was adopted to identify the sustainability status of those national nature reserves. It was found that currently (2010) 45% of NRRs were in the condition of ecological deficit. In terms of dynamic changes in EF and BC, only 16% of NRRs were sustainable. The 124 national nature reserves that were in the red light state were mainly distributed in Anhui Province, Chongqing City, Hunan, Guizhou, Fujian, Shandong Province, and Inner Mongolia. The percentage of nature reserves at the red light state in these areas were 83.3%, 66.7%, 64.7%, 62.5%, 58.3%, 57.1%, and 56.5%, respectively. The reserves in the red light state should be included in the priority concern level and should be strictly controlled in terms of population growth and the intensity of exploitation. The results of this study will provide more effective data for reference and for decision making support in nature reserve protection.
    Electronic ISSN: 2071-1050
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by MDPI Publishing
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