ALBERT

Description: Information on potential cough triggers including environmental irritants is vital for successful management of chronic cough in patients. We investigated the relationship between ambient levels of particulate polycyclic aromatic hydrocarbons (PAH), nitrogen dioxide (NO2) and sulphur dioxide (SO2) exposures with cough prevalence. Eighty-three adult patients, who had been physician diagnosed with at least asthma, cough variant asthma and/or atopic cough, were divided into asthma and non-asthma groups. They recorded daily cough symptoms during 4 January–30 June 2011 study period while daily samples of total suspended particles were simultaneously collected by use of glass fiber filters and the particulate PAH content determined by high performance liquid chromatography coupled with a fluorescence detector. Ambient concentrations of NO2 and SO2 were obtained from a local monitoring site. Logistic regression models using generalized estimating equations were used to determine population-averaged estimates of association between cough prevalence and ambient pollutant exposures for the two groups. Fully adjusted odds ratios from single pollutant models were 1.083 (95% confidence interval (CI): 1.029, 1.140) and 1.097 (95% CI: 1.016, 1.185) per 0.57 ng/m3 for lag2 PAH exposure, while only for asthma group had significant associations with NO2 and SO2 exposures for both lag2 and lag02. Similar associations were observed in multipollutant models. This finding suggests that ambient PAH, NO2, and SO2 exposure even at low levels is related to cough prevalence in adult chronic cough patients and may be considered as aggravating factor during clinical management of the condition.

Description: Options to tackle the sustainability challenges faced in the production of rice, including global and local environmental perspectives, need to be discussed. Here, the global warming potential, water consumption and cumulative energy demand were analyzed using a life-cycle assessment to highlight the sustainability aspects of rice production in Taiwan, where a mixed organic and conventional rice production with a dual cropping system is practiced. The results show that the conventional farming method practiced in Houbi district contributes less to global warming and annual water consumption and consumes less energy than the organic method practiced in Luoshan village on a grain weight basis. It is also more lucrative for farmers because of the higher rice yield. Considering the yield ratio based on the data from two districts, the regional characteristics are more responsible for these differences. Giving up dual cropping to avail water to other sectors by fallowing during the second cropping season is preferable from the GHG emission and productivity perspectives. However, because water shortages usually occur in the first cropping season, it is more realistic to fallow during the first cropping season when domestic and other industrial users have the higher priority. The results presented here can serve as the foundation for exploring the possibilities of options, such as new biorefinery technologies and water allocation policies, in relation to influences on GHG emissions and the national self-sufficiency of rice.

Description: A shrimp farmer in Taiwan practices innovation through trial-and-error for better income and a better environment, but such farmer-based innovation sometimes fails because the biological mechanism is unclear. Systematic field experimentation and laboratory research are often too costly, and simulating ground conditions is often too challenging. To solve this dilemma, we propose a decision support framework that explicitly utilizes farmer experiential knowledge through a participatory approach to alternatively estimate prospective change in shrimp farming productivity, and to co-design options for improvement. Data obtained from the farmer enable us to quantitatively analyze the production cost and greenhouse gas (GHG) emission with a life cycle assessment (LCA) methodology. We used semi-quantitative graphical representations of indifference curves and mixing triangles to compare and show better options for the farmer. Our results empower the farmer to make decisions more systematically and reliably based on the frequency of heterotrophic bacteria application and the revision of feed input. We argue that experiential knowledge may be less accurate due to its dependence on varying levels of farmer experience, but this knowledge is a reasonable alternative for immediate decision-making. More importantly, our developed framework advances the scope of LCA application to support practically important yet scientifically uncertain cases.

Description: HIV-1 epidemics in Caribbean countries, including Puerto Rico, have been reported to be almost exclusively associated with the subtype B virus (HIV-1B). However, while HIV infections associated with other clades have been only sporadically reported, no organized data exist to accurately assess the prevalence of non-subtype B HIV-1 infection. We analyzed the nucleotide sequence data of the HIV pol gene associated with HIV isolates from Puerto Rican patients. The sequences (n = 945) were obtained from our “HIV Genotyping” test file, which has been generated over a period of 14 years (2001–2014). REGA subtyping tool found the following subtypes: B (90%), B-like (3%), B/D recombinant (6%), and D/B recombinant (0.6%). Though there were fewer cases, the following subtypes were also found (in the given proportions): A1B (0.3%), BF1 (0.2%), subtype A (01-AE) (0.1%), subtype A (A2) (0.1%), subtype F (12BF) (0.1%), CRF-39 BF-like (0.1%), and others (0.1%). Some of the recombinants were identified as early as 2001. Although the HIV epidemic in Puerto Rico is primarily associated with HIV-1B virus, our analysis uncovered the presence of other subtypes. There was no indication of subtype C, which has been predominantly associated with heterosexual transmission in other parts of the world.

Description: Coastal areas in Minami-soma City, Fukushima, Japan, were seriously damaged by radioactive contamination from the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident that caused multiple pollution by tsunami and radionuclide exposure, after the Great East Japan Earthquake, on 11 March 2011. Some areas will remain no-go zones because radiation levels remain high. In Minami-soma, only 26 percent of decontamination work had been finished by the end of July in 2015. Here, we report the characterization of microbial mats and salt found on flooded paddy fields at Karasuzaki, Minami-soma City, Fukushima Prefecture, Japan which have been heavily contaminated by radionuclides, especially by Cs (134Cs, 137Cs), 40K, Sr (89Sr, 90Sr), and 91 or 95Zr even though it is more than 30 km north of the FDNPP. We document the mineralogy, the chemistry, and the micro-morphology, using a combination of micro techniques. The microbial mats were found to consist of diatoms with mineralized halite and gypsum by using X-ray diffraction (XRD). Particular elements concentrated in microbial mats were detected using scanning electron microscopy equipped with energy dispersive spectroscopy (SEM-EDS) and X-ray fluorescence (XRF). The objective of this contribution is to illustrate the ability of various diatoms associated with minerals and microorganisms which are capable of absorbing both radionuclides and stable isotopes from polluted paddy soils in extreme conditions. Ge semiconductor analysis of the microbial mats detected 134Cs, 137Cs, and 40K without 131I in 2012 and in 2013. Quantitative analysis associated with the elemental content maps by SEM-EDS indicated the possibility of absorption of radionuclide and stable isotope elements from polluted paddy soils in Fukushima Prefecture. In addition, radionuclides were detected in solar salts made of contaminated sea water collected from the Karasuzaki ocean bath, Minami-soma, Fukushima in 2015, showing high Zr content associated with 137Cs and 40K without 131I. The results obtained here provide evidence of the ability of microorganisms to grow in this salty contaminated environment and to immobilize radionuclides. It is possible that the capability of radioactive immobilization can be used to counteract the disastrous effects of radionuclide-polluted paddy soils.

Description: We propose a novel fast-responding and paintable pressure-sensitive paint (PSP) based on polymer particles, i.e. polymer-particle (pp-)PSP. As a fast-responding PSP, polymer-ceramic (PC-)PSP is widely studied. Since PC-PSP generally consists of titanium (IV) oxide (TiO2) particles, a large reduction in the luminescent intensity will occur due to the photocatalytic action of TiO2. We propose the usage of polymer particles instead of TiO2 particles to prevent the reduction in the luminescent intensity. Here, we fabricate pp-PSP based on the polystyrene particle with a diameter of 1 μm, and investigate the pressure- and temperature-sensitives, the response time, and the photostability. The performances of pp-PSP are compared with those of PC-PSP, indicating the high photostability with the other characteristics comparable to PC-PSP.

Description: IJERPH, Vol. 15, Pages 1697: Sanger and Next Generation Sequencing Approaches to Evaluate HIV-1 Virus in Blood Compartments International Journal of Environmental Research and Public Health doi: 10.3390/ijerph15081697 Authors: Andrea Arias Pablo López Raphael Sánchez Yasuhiro Yamamura Vanessa Rivera-Amill The implementation of antiretroviral treatment combined with the monitoring of drug resistance mutations improves the quality of life of HIV-1 positive patients. The drug resistance mutation patterns and viral genotypes are currently analyzed by DNA sequencing of the virus in the plasma of patients. However, the virus compartmentalizes, and different T cell subsets may harbor distinct viral subsets. In this study, we compared the patterns of HIV distribution in cell-free (blood plasma) and cell-associated viruses (peripheral blood mononuclear cells, PBMCs) derived from ART-treated patients by using Sanger sequencing- and Next-Generation sequencing-based HIV assay. CD4+CD45RA−RO+ memory T-cells were isolated from PBMCs using a BD FACSAria instrument. HIV pol (protease and reverse transcriptase) was RT-PCR or PCR amplified from the plasma and the T-cell subset, respectively. Sequences were obtained using Sanger sequencing and Next-Generation Sequencing (NGS). Sanger sequences were aligned and edited using RECall software (beta v3.03). The Stanford HIV database was used to evaluate drug resistance mutations. Illumina MiSeq platform and HyDRA Web were used to generate and analyze NGS data, respectively. Our results show a high correlation between Sanger sequencing and NGS results. However, some major and minor drug resistance mutations were only observed by NGS, albeit at different frequencies. Analysis of low-frequency drugs resistance mutations and virus distribution in the blood compartments may provide information to allow a more sustainable response to therapy and better disease management.

Description: Remote Sensing, Vol. 10, Pages 1430: Monitoring of Thermal Activity at the Hatchobaru–Otake Geothermal Area in Japan Using Multi-Source Satellite Images—With Comparisons of Methods, and Solar and Seasonal Effects Remote Sensing doi: 10.3390/rs10091430 Authors: Md. Bodruddoza Mia Yasuhiro Fujimitsu Jun Nishijima The Hatchobaru–Otake (HO) geothermal field is proximal to the Kuju volcano on Kyushu, Japan. There are currently three geothermal power plants operating within this geothermal field. Herein, we explore the thermal status of the HO geothermal area using ASTER thermal infrared data to monitor heat losses from 2009 to 2017. We assessed the solar effects and seasonal variation on heat losses based on day- and night-time Landsat thermal infrared images, and compared three conventional methods of land surface temperature (LST) measurements. The normalized difference vegetation index threshold method of emissivity, the split window algorithm for LST, and the Stefan–Boltzmann equation for radiative heat flux (RHF) were used to determine the heat loss within the study area. The radiative heat loss (RHL) was 0.36 MW, 38.61 MW, and 29.14 MW in 2009, 2013, and 2017, respectively, from the HO geothermal field. The highest anomaly in RHF was recorded in 2013, while the lowest was in 2009. The RHLs were higher from Otake than from the Hatchobaru thermal area in the year of 2013 (~31%) and 2017 (~78%). The seasonal variation in the RHLs based on all three LST estimation methods had a similar pattern, with the highest RHL (about 383–451 MW) in spring and the lowest (about 10–222 MW) in autumn for the daytime images from the HO geothermal field. In the nighttime images, the highest RHL was about 35–67 MW in autumn and the lowest was about 1–3 MW in spring, based on the three LST methods for RHFs. The highest RHL was about 35–42 MW in spring (day) and 3–7 MW in autumn (night) from the Hatchobaru thermal area, analyzed separately. Similarly, the highest RHL was about 22–25 MW in spring (day) and 4–5 MW in winter (night) from the Otake thermal area. The seasonal variation was greatly influenced by the regional ambient temperature. We also observed that clouds had a huge effect, with the highest values for both LST and RHF recorded below clouds on an autumn day. Overall, we obtained higher LSTs at nighttime and lower LSTs during the day from the improved mono-window algorithm than the split window algorithms for all of the seasons. The heat losses were also higher for the improved mono-window algorithm than the split window algorithms, based on the LST nighttime thermal infrared data. Considering the error level of the LST methods and Landsat 8 band 11, this study recommends the IWM method for LST using the Landsat 8 band 10 data. This study also suggests that both the nighttime ASTER and Landsat 8 thermal infrared data could be effective for monitoring the thermal status of the HO geothermal area, given that data is available for the entire period.

Description: IJERPH, Vol. 15, Pages 1417: Effects of Railway Elevation, Operation of a New Station, and Earthquakes on Railway Noise Annoyance in Kumamoto, Japan International Journal of Environmental Research and Public Health doi: 10.3390/ijerph15071417 Authors: Yasuhiro Murakami Takashi Yano Makoto Morinaga Shigenori Yokoshima This study investigated the effects of railway elevation, operation of a new station, and earthquakes on railway noise annoyance in two areas along a conventional railway line (CRL) adjacent to the Kyushu Shinkansen line: the north area with the CRL elevation and the south area with the operation of the new station, both of which occurred in March 2016. In April 2016, Kumamoto region was struck by a series of large earthquakes, prompting their inclusion in this study, as frequent aftershocks with loud ground rumbling might make people more sensitive to railway noise and vibration. Socioacoustic surveys were performed in both areas before and after the earthquakes. Because very few respondents in the north area reported that they were “highly annoyed,” further analysis was conducted on data from the south area. The exposure–annoyance relationship was found to be significantly higher in 2017 than in 2011 despite lower noise exposure. Multiple logistic regression analysis showed that Lden, noise sensitivity, and serious damage by the earthquakes in addition to the operation of the new station significantly affected the annoyance in both detached and apartment houses. However, when the earthquakes caused minimal damage, they did not significantly affect annoyance.

Description: Sensors, Vol. 17, Pages 1752: Development of Wearable Sheet-Type Shear Force Sensor and Measurement System that is Insusceptible to Temperature and Pressure Sensors doi: 10.3390/s17081752 Authors: Shigeru Toyama Yasuhiro Tanaka Satoshi Shirogane Takashi Nakamura Tokio Umino Ryo Uehara Takuma Okamoto Hiroshi Igarashi A sheet-type shear force sensor and a measurement system for the sensor were developed. The sensor has an original structure where a liquid electrolyte is filled in a space composed of two electrode-patterned polymer films and an elastic rubber ring. When a shear force is applied on the surface of the sensor, the two electrode-patterned films mutually move so that the distance between the internal electrodes of the sensor changes, resulting in current increase or decrease between the electrodes. Therefore, the shear force can be calculated by monitoring the current between the electrodes. Moreover, it is possible to measure two-dimensional shear force given that the sensor has multiple electrodes. The diameter and thickness of the sensor head were 10 mm and 0.7 mm, respectively. Additionally, we also developed a measurement system that drives the sensor, corrects the baseline of the raw sensor output, displays data, and stores data as a computer file. Though the raw sensor output was considerably affected by the surrounding temperature, the influence of temperature was drastically decreased by introducing a simple arithmetical calculation. Moreover, the influence of pressure simultaneously decreased after the same calculation process. A demonstrative measurement using the sensor revealed the practical usefulness for on-site monitoring.