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  • 1
    Publication Date: 2015-12-30
    Description: Gram-positive bacteria Bacillus subtilis B068150 has been used as a biocontrol agent against the pathogen Fusarium oxysporum cucumerinum. Cucumber was grown in three soils with strain B068150 inoculated in a greenhouse for 90 days, and the colonization ability of strain B068150 in cucumber rhizosphere and non-rhizosphere soils was determined. Changes in total bacteria and fungi community composition and structures using denaturing gradient gel electrophoresis (DGGE) and sequencing were determined. Colony counts showed that B068150 colonization in the rhizosphere was significantly higher (p 〈 0.001) than in non-rhizosphere soils. Based on our data, the introduction of B. bacillus B068150 did not change the diversity of microbial communities significantly in the rhizosphere of three soils. Our data showed that population density of B068150 in clay soil had a significant negative correlation on bacterial diversity in cucumber rhizosphere in comparison to loam and sandy soils, suggesting that the impact of B068150 might be soil specific.
    Electronic ISSN: 2077-0472
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by MDPI
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  • 2
    Publication Date: 2019
    Description: Oilseed rape (Brassica napus) is the second largest oilseed crop worldwide. As an architecture component of B. napus, thickness of pod canopy (TPC) plays an important role in yield formation, especially under high-density cultivation conditions. However, the mechanisms underlying the regulation of TPC remain unclear. RNA and microRNA (miRNA) profiling of two groups of B. napus lines with significantly different TPC at the bolting with a tiny bud stage revealed differential expressions of numerous genes involved in nitrogen-related pathways. Expression of several nitrogen-related response genes, including ASP5, ASP2, ASN3, ATCYSC1, PAL2, APT2, CRTISO, and COX15, was dramatically changed in the thick TPC lines compared to those in the thin TPC lines. Differentially expressed miRNAs also included many involved in nitrogen-related pathways. Expression of most target genes was negatively associated with corresponding miRNAs, such as miR159, miR6029, and miR827. In addition, 12 (including miR319, miR845, and miR158) differentially expressed miRNAs between two plant tissues sampled (stem apex and flower bud) were identified, implying that they might have roles in determining overall plant architecture. These results suggest that nitrogen signaling may play a pivotal role in regulating TPC in B. napus.
    Electronic ISSN: 2073-4425
    Topics: Biology
    Published by MDPI
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  • 3
    Publication Date: 2018
    Description: Noise reduction of underwater acoustic signals is of great significance in the fields of military and ocean exploration. Based on the adaptive decomposition characteristic of uniform phase empirical mode decomposition (UPEMD), a noise reduction method for underwater acoustic signals is proposed, which combines amplitude-aware permutation entropy (AAPE) and Pearson correlation coefficient (PCC). UPEMD is a recently proposed improved empirical mode decomposition (EMD) algorithm that alleviates the mode splitting and residual noise effects of EMD. AAPE is a tool to quantify the information content of nonlinear time series. Unlike permutation entropy (PE), AAPE can reflect the amplitude information on time series. Firstly, the original signal is decomposed into a series of intrinsic mode functions (IMFs) by UPEMD. The AAPE of each IMF is calculated. The modes are separated into high-frequency IMFs and low-frequency IMFs, and all low-frequency IMFs are determined as useful IMFs (UIMFs). Then, the PCC between the high-frequency IMF with the smallest AAPE and the original signal is calculated. If PCC is greater than the threshold, the IMF is also determined as a UIMF. Finally, all UIMFs are reconstructed and the denoised signal is obtained. Chaotic signals with different signal-to-noise ratios (SNRs) are used for denoising experiments. Compared with EMD and extreme-point symmetric mode decomposition (ESMD), the proposed method has higher SNR and smaller root mean square error (RMSE). The proposed method is applied to noise reduction of real underwater acoustic signals. The results show that the method can further eliminate noise and the chaotic attractors are smoother and clearer.
    Electronic ISSN: 1099-4300
    Topics: Chemistry and Pharmacology , Physics
    Published by MDPI
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  • 4
    Publication Date: 2018
    Description: Owing to the problems that imperfect decomposition process of empirical mode decomposition (EMD) denoising algorithm and poor self-adaptability, it will be extremely difficult to reduce the noise of signal. In this paper, a noise reduction method of underwater acoustic signal denoising based on complete ensemble empirical mode decomposition with adaptive noise (CEEMDAN), effort-to-compress complexity (ETC), refined composite multiscale dispersion entropy (RCMDE) and wavelet threshold denoising is proposed. Firstly, the original signal is decomposed into several IMFs by CEEMDAN and noise IMFs can be identified according to the ETC of IMFs. Then, calculating the RCMDE of remaining IMFs, these IMFs are divided into three kinds of IMFs by RCMDE, namely noise-dominant IMFs, real signal-dominant IMFs, real IMFs. Finally, noise IMFs are removed, wavelet soft threshold denoising is applied to noise-dominant IMFs and real signal-dominant IMFs. The denoised signal can be obtained by combining the real IMFs with the denoised IMFs after wavelet soft threshold denoising. Chaotic signals with different signal-to-noise ratio (SNR) are used for denoising experiments by comparing with EMD_MSE_WSTD and EEMD_DE_WSTD, it shows that the proposed algorithm has higher SNR and smaller root mean square error (RMSE). In order to further verify the effectiveness of the proposed method, which is applied to noise reduction of real underwater acoustic signals. The results show that the denoised underwater acoustic signals not only eliminate noise interference also restore the topological structure of the chaotic attractors more clearly, which lays a foundation for the further processing of underwater acoustic signals.
    Electronic ISSN: 1099-4300
    Topics: Chemistry and Pharmacology , Physics
    Published by MDPI
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  • 5
    Publication Date: 2018
    Description: Land surface temperature (LST) is an important parameter to evaluate environmental changes. In this paper, time series analysis was conducted to estimate the interannual variations in global LST from 2001 to 2016 based on moderate resolution imaging spectroradiometer (MODIS) LST, and normalized difference vegetation index (NDVI) products and fine particulate matter (PM2.5) data from the Atmospheric Composition Analysis Group. The results showed that LST, seasonally integrated normalized difference vegetation index (SINDVI), and PM2.5 increased by 0.17 K, 0.04, and 1.02 μg/m3 in the period of 2001–2016, respectively. During the past 16 years, LST showed an increasing trend in most areas, with two peaks of 1.58 K and 1.85 K at 72°N and 48°S, respectively. Marked warming also appeared in the Arctic. On the contrary, remarkable decrease in LST occurred in Antarctic. In most parts of the world, LST was affected by the variation in vegetation cover and air pollutant, which can be detected by the satellite. In the Northern Hemisphere, positive relations between SINDVI and LST were found; however, in the Southern Hemisphere, negative correlations were detected. The impact of PM2.5 on LST was more complex. On the whole, LST increased with a small increase in PM2.5 concentrations but decreased with a marked increase in PM2.5. The study provides insights on the complex relationship between vegetation cover, air pollution, and land surface temperature.
    Electronic ISSN: 2072-4292
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Published by MDPI
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  • 6
    Publication Date: 2019
    Description: Sea environment complexity and underwater acoustic channels make it hard to extract features of ship-radiated noise signals. This paper presents a novel feature extraction method using the advantages of variational mode decomposition (VMD), fluctuation-based dispersion entropy (FDE) and self-organizing feature map (SOM). Firstly, VMD decomposition of the original signal is used to get a group of bandwidth-limited intrinsic mode functions (IMFs). Then, the difference between the FDE of each IMF and the original signal is calculated, respectively; the IMF with the smallest difference (SIMF) is selected to calculate the FDE as the feature vector. Finally, the characteristic vectors are sent to the SOM classifier to categorize the original signal. The proposed method is applied to feature extraction of real ship-radiated noise signals. The results show that this method is more precise for ship-radiated noise signals feature extraction.
    Electronic ISSN: 1099-4300
    Topics: Chemistry and Pharmacology , Physics
    Published by MDPI
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  • 7
    Publication Date: 2019
    Description: The approach to improve the output power of piezoelectric energy harvester is one of the current research hotspots. In the case where some sources have two or more discrete vibration frequencies, this paper proposed three types of magnetically coupled multi-frequency hybrid energy harvesters (MHEHs) to capture vibration energy composed of two discrete frequencies. Electromechanical coupling models were established to analyze the magnetic forces, and to evaluate the power generation characteristics, which were verified by the experimental test. The optimal structure was selected through the comparison. With 2 m/s2 excitation acceleration, the optimal peak output power was 2.96 mW at 23.6 Hz and 4.76 mW at 32.8 Hz, respectively. The superiority of hybrid energy harvesting mechanism was demonstrated. The influences of initial center-to-center distances between two magnets and length of cantilever beam on output power were also studied. At last, the frequency sweep test was conducted. Both theoretical and experimental analyses indicated that the proposed MHEH produced more electric power over a larger operating bandwidth.
    Electronic ISSN: 1424-8220
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Published by MDPI
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  • 8
    Publication Date: 2019
    Description: Hemp is a Pb-tolerant and Pb-accumulating plant and the study of its tolerance mechanisms could facilitate the breeding of hemp with enhanced Pb tolerance and accumulation. In the present study, we took advantage of sequential window acquisition of all theoretical mass spectra (SWATH) technology to study the difference in proteomics between the leaves of Pb-tolerant seed-type hemp variety Bamahuoma (BM) and the Pb-sensitive fiber-type hemp variety Yunma 1 (Y1) under Pb stress (3 g/kg soil). A total of 63 and 372 proteins differentially expressed under Pb stress relative to control conditions were identified with liquid chromatography electro spray ionization tandem mass spectrometry in BM and Y1, respectively; with each of these proteins being classified into 14 categories. Hemp adapted to Pb stress by: accelerating adenosine triphosphate (ATP) metabolism; enhancing respiration, light absorption and light energy transfer; promoting assimilation of intercellular nitrogen (N) and carbon (C); eliminating reactive oxygen species; regulating stomatal development and closure; improving exchange of water and CO2 in leaves; promoting intercellular transport; preventing aggregation of unfolded proteins; degrading misfolded proteins; and increasing the transmembrane transport of ATP in chloroplasts. Our results provide an important reference protein and gene information for future molecular studies into the resistance and accumulation of Pb in hemp.
    Electronic ISSN: 2073-4425
    Topics: Biology
    Published by MDPI
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  • 9
    Publication Date: 2019
    Description: To improve the recognition accuracy of ship-radiated noise, a feature extraction method based on regenerated phase-shifted sinusoid-assisted empirical mode decomposition (RPSEMD), mutual information (MI), and differential symbolic entropy (DSE) is proposed in this paper. RPSEMD is an improved empirical mode decomposition (EMD) that alleviates the mode mixing problem of EMD. DSE is a new tool to quantify the complexity of nonlinear time series. It not only has high computational efficiency, but also can measure the nonlinear complexity of short time series. Firstly, the ship-radiated noise is decomposed into a series of intrinsic mode functions (IMFs) by RPSEMD, and the DSE of each IMF is calculated. Then, the MI between each IMF and the original signal is calculated; the sum of MIs is taken as the denominator; and each normalized MI (norMI) is obtained. Finally, each norMI is used as the weight coefficient to weight the corresponding DSE, and the weighted DSE (WDSE) is obtained. The WDSEs are sent into the support vector machine (SVM) classifier to classify and recognize three types of ship-radiated noise. The experimental results demonstrate that the recognition rate of the proposed method reaches 98.3333%. Consequently, the proposed WDSE method can effectively achieve the classification of ships.
    Electronic ISSN: 1099-4300
    Topics: Chemistry and Pharmacology , Physics
    Published by MDPI
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  • 10
    Publication Date: 2018
    Description: The matter of success in forecasting precipitation is of great significance to flood control and drought relief, and water resources planning and management. For the nonlinear problem in forecasting precipitation time series, a hybrid prediction model based on variational mode decomposition (VMD) coupled with extreme learning machine (ELM) is proposed to reduce the difficulty in modeling monthly precipitation forecasting and improve the prediction accuracy. The monthly precipitation data in the past 60 years from Yan’an City and Huashan Mountain, Shaanxi Province, are used as cases to test this new hybrid model. First, the nonstationary monthly precipitation time series are decomposed into several relatively stable intrinsic mode functions (IMFs) by using VMD. Then, an ELM prediction model is established for each IMF. Next, the predicted values of these components are accumulated to obtain the final prediction results. Finally, three predictive indicators are adopted to measure the prediction accuracy of the proposed hybrid model, back propagation (BP) neural network, Elman neural network (Elman), ELM, and EMD-ELM models: mean absolute error (MAE), root mean squared error (RMSE), and mean absolute percentage error (MAPE). The experimental simulation results show that the proposed hybrid model has higher prediction accuracy and can be used to predict the monthly precipitation time series.
    Electronic ISSN: 2078-2489
    Topics: Computer Science
    Published by MDPI
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