ALBERT

Description: Increasing evidence suggests that overlapping genes are much more common in eukaryotic genomes than previously thought. These different-strand overlapping genes are potential sense–antisense (SAS) pairs, which might have regulatory effects on each other. In the present study, we identified the SAS loci in the equine genome using previously generated stranded, paired-end RNA sequencing data from the equine chorioallantois. We identified a total of 1261 overlapping loci. The ratio of the number of overlapping regions to chromosomal length was numerically higher on chromosome 11 followed by chromosomes 13 and 12. These results show that overlapping transcription is distributed throughout the equine genome, but that distributions differ for each chromosome. Next, we evaluated the expression patterns of SAS pairs during the course of gestation. The sense and antisense genes showed an overall positive correlation between the sense and antisense pairs. We further provide a list of SAS pairs with both positive and negative correlation in their expression patterns throughout gestation. This study characterizes the landscape of sense and antisense gene expression in the placenta for the first time and provides a resource that will enable researchers to elucidate the mechanisms of sense/antisense regulation during pregnancy.

Description: High blood urea nitrogen (BUN) decreases fertility of several mammals; however, the mechanisms have not been investigated in mares. We developed an experimental model to elevate BUN, with urea and control treatments (7 mares/treatment), in a crossover design. Urea-treatment consisted of a loading dose of urea (0.03 g/kg of body weight (BW)) and urea injections over 6 hours (0.03 g/kg of BW/h). Control mares received the same volume of saline solution. Blood samples were collected to measure BUN. Uterine and vaginal pH were evaluated after the last intravenous infusion, then endometrial biopsies were collected for RNA-sequencing with a HiSeq 4000. Cuffdiff (2.2.1) was used to identify the differentially expressed genes (DEG) between urea and control groups (false discovery rate-adjusted p-value 〈 0.1). There was a significant increase in BUN and a decrease of uterine pH in the urea group compared to the control group. A total of 193 genes were DEG between the urea and control groups, with five genes identified as upstream regulators (ETV4, EGF, EHF, IRS2, and SGK1). The DEG were predicted to be related to cell pH, ion homeostasis, changes in epithelial tissue, and solute carriers. Changes in gene expression reveal alterations in endometrial function that could be associated with adverse effects on fertility of mares.

Description: The different generations of calc-silicate assemblages formed during sequential metasomatic events make the Campiglia Marittima magmatic–hydrothermal system a prominent case study to investigate the mobility of rare earth element (REE) and other trace elements. These mineralogical assemblages also provide information about the nature and source of metasomatizing fluids. Petrographic and geochemical investigations of granite, endoskarn, and exoskarn bodies provide evidence for the contribution of metasomatizing fluids from an external source. The granitic pluton underwent intense metasomatism during post-magmatic fluid–rock interaction processes. The system was initially affected by a metasomatic event characterized by circulation of K-rich and Ca(-Mg)-rich fluids. A potassic metasomatic event led to the complete replacement of magmatic biotite, plagioclase, and ilmenite, promoting major element mobilization and crystallization of K-feldspar, phlogopite, chlorite, titanite, and rutile. The process resulted in significant gain of K, Rb, Ba, and Sr, accompanied by loss of Fe and Na, with metals such as Cu, Zn, Sn, W, and Tl showing significant mobility. Concurrently, the increasing fluid acidity, due to interaction with Ca-rich fluids, resulted in a diffuse Ca-metasomatism. During this stage, a wide variety of calc-silicates formed (diopside, titanite, vesuvianite, garnet, and allanite), throughout the granite body, along granite joints, and at the carbonate–granite contact. In the following stage, Ca-F-rich fluids triggered the acidic metasomatism of accessory minerals and the mobilization of high-field-strength elements (HFSE) and REE. This stage is characterized by the exchange of major elements (Ti, Ca, Fe, Al) with HFSE and REE in the forming metasomatic minerals (i.e., titanite, vesuvianite) and the crystallization of HFSE-REE minerals. Moreover, the observed textural disequilibrium of newly formed minerals (pseudomorphs, patchy zoning, dissolution/reprecipitation textures) suggests the evolution of metasomatizing fluids towards more acidic conditions at lower temperatures. In summary, the selective mobilization of chemical components was related to a shift in fluid composition, pH, and temperature. This study emphasizes the importance of relating field studies and petrographic observations to detailed mineral compositions, leading to the construction of litho-geochemical models for element mobilization in crustal magmatic-hydrothermal settings.

Description: We aimed to describe and evaluate the implementation of a home-based exercise program among community-dwelling adults aged ≥65 years. In an observational study, the twelve-week program was implemented in a community setting. The implementation plan consisted of dialogues with healthcare professionals and older adults, development of an implementation protocol, recruitment of participants, program implementation, and implementation evaluation. The dialogues consisted of a Delphi survey among healthcare professionals, and of individual and group meetings among older adults. The implementation of the program was evaluated using the framework model RE-AIM. In the dialogues with healthcare professionals and older adults, it was found that negative consequences of a fall and positive effects of preventing a fall should be emphasized to older adults, in order to get them engaged in fall prevention activities. A total of 450 older adults enrolled in the study, of which 238 started the program. The process evaluation showed that the majority of older adults were recruited by a community nurse. Also, a good collaboration between the research team and the local primary healthcare providers was accomplished, which was important in the recruitment. Future fall prevention studies may use this information in order to translate an intervention in a research project into a community-based program.

Description: This observational study was conducted to determine which factors are associated with frequent participation in a home-based exercise program. The effects of frequent participation on health-related outcomes over time are investigated, as well. Community-dwelling adults aged ≥65 years participated in a twelve-week home-based exercise program. The program consisted of an instruction book with exercises that were performed individually at home. Frequent participation was classified as performing exercises of the instruction book daily or a few days a week during the study period. A logistic regression analysis was performed to determine the association between factors (i.e., demographic and health-related characteristics) and frequent participation. Furthermore, to investigate the effects of frequent participation on health-related outcomes, generalized linear and logistic regression models were built. A total of 238 participants (mean age 81.1 years (SD ± 6.7), 71% female) were included in the study. Frequent participation during the study period was indicated by fifty-two percent of participants. Analyses showed that a higher degree of pain (OR: 1.02, 95% CI: 1.–1.04) was associated with frequent participation. In addition, the effect of frequent participation over time was a significant improvement in current health perceptions (B: 4.46, SE: 1.99).

Description: The Baccu Locci mine area is located in a sector of the Variscan Nappe zone of Sardinia (the Baccu Locci shear zone) that hosts several type of ore deposits mined until the first half of the last century. The orebodies consist of lenses of Zn–Cu sulphides, once interpreted as stratabound, and Qtz–As–Pb sulphide ± gold veins; the implication of structural controls in their origin were previously misinterpreted or not considered. Detailed field mapping, structural analyses, and ore mineralogy allowed for unraveling how different ore parageneses are superimposed each other and to recognize different relationships with the Variscan structures. The sulphide lenses are parallel to the mylonitic foliation, hosted in the hinges of minor order upright antiforms that acted as traps for hydrothermal fluids. The Qtz–As–Pb sulphide veins crosscut the sulphide lenses and are hosted in large dilatational jogs developed in the hanging wall of dextral-reverse faults, whose geometry is influenced by the attitude of reverse limbs of late Variscan folds. The ores in the Baccu Locci shear zone are best interpreted as Variscan orogenic gold-type; veins display mutual crosscutting relationships with mafic dikes dated in the same district at 302 ± 0.2 Ma, a reliable age for the mineralizing events in the area.

Description: Muon absorption radiography is an imaging technique based on the measurement of the absorption of cosmic ray muons. This technique has recently been used successfully to investigate the presence of unknown cavities in the Bourbon Gallery in Naples and in the Chephren Pyramid at Cairo. The MIMA detector (Muon Imaging for Mining and Archaeology) is a prototype muon tracker for muon radiography for application in the fields of archaelogy and mining. It is made of three pairs of X-Y planes each consisting of 21 scintillator bars with a silicon photomultiplier readout. The detector is compact, robust, easily transportable, and has a low power consumption: all of which makes the detector ideal for measurements in confined and isolated environments. With this detector, a measurement from inside the Temperino mine in the San Silvestro archaeo-mining park in Tuscany was performed. The park includes about 25 km of mining tunnels arranged on several levels that have been exploited from the Etruscan time. The measured muon absorption was compared to the simulated one, obtained from the information provided by 3D laser scanner measurements and cartographic maps of the mountain above the mine, in order to obtain information about the average density of the rock. This allowed one to confirm the presence of a partially accessible exploitation opening and provided some hints regarding the presence of a high-density body within the rock.

Description: This work addresses the problem of mitigating the effects of the cogging torque in permanent magnet synchronous motors, particularly brushless motors, which is a main issue in precision electric drive applications. In this work, a method for mitigating the effects of the cogging torque is proposed, based on the use of a nonlinear automatic control technique known as feedback linearization that is ideal for underactuated dynamic systems. The aim of this work is to present an alternative to classic solutions based on the physical modification of the electrical machine to try to suppress the natural interaction between the permanent magnets and the teeth of the stator slots. Such modifications of electric machines are often expensive because they require customized procedures, while the proposed method does not require any modification of the electric drive. With respect to other algorithmic-based solutions for cogging torque reduction, the proposed control technique is scalable to different motor parameters, deterministic, and robust, and hence easy to use and verify for safety-critical applications. As an application case example, the work reports the reduction of the oscillations for the angular position control of a permanent magnet synchronous motor vs. classic PI (proportional-integrative) cascaded control. Moreover, the proposed algorithm is suitable to be implemented in low-cost embedded control units.

Description: A hybrid solid desiccant cooling system (SDCS), which combines a solid desiccant system and a vapor compression system, is considered to be an excellent alternative for commercial and residential air conditioning systems. In this study, a solar-assisted hybrid SDCS system was developed in which solar-heated water is used as an additional heat source for the regeneration process, in addition to recovering heat from the condenser of an integrated heat pump. A solar thermal collector sub-system is used to generate solar regeneration water. Experiments were conducted in the typically hot and humid weather of Taichung, Taiwan, from the spring to fall seasons. The experimental results show that the overall performance of the system in terms of power consumption can be enhanced by approximately 10% by integrating a solar-heated water heat exchanger in comparison to the hybrid SDCS system. The results show that the system performs better when the outdoor humidity ratio is large. In addition, regarding the effect of ambient temperature on the coefficient of performance (COP) of the systems, a critical value of outdoor temperature exists. The COP of the systems gradually rises with the increase in ambient temperature. However, when the ambient temperature is greater than the critical value, the COP gradually decreases with the increase in ambient temperature. The critical outdoor temperature of the hybrid SDCS is from 26 °C to 27 °C, and the critical temperature of the solar-assisted hybrid SDCS is from 27 °C to 30 °C.