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  • International Union of Crystallography (IUCr)  (28)
  • PANGAEA
  • Copernicus
  • 1995-1999  (40)
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Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 55 (1999), S. 403-407 
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Human lactoferrin (hLf) has considerable potential as a therapeutic agent. Overexpression of hLf in the fungus Aspergillus awamori has resulted in the availability of very large quantities of this protein. Here, the three-dimensional structure of the recombinant hLf has been determined by X-ray crystallography at a resolution of 2.2 Å. The final model, comprising 5339 protein atoms (residues 1–691, 294 solvent molecules, two Fe3+and two CO_3^{2-} ions), gives an R factor of 0.181 (free R = 0.274) after refinement against 32231 reflections in the resolution range 10–2.2 Å. Superposition of the recombinant hLf structure onto the native milk hLf structure shows a very high level of correspondence; the main-chain atoms for the entire polypeptide can be superimposed with an r.m.s. deviation of only 0.3 Å and there are no significant differences in side-chain conformations or in the iron-binding sites. Dynamic properties, as measured by B-value distributions or iron-release kinetics, also agree closely. This shows that the structure of the protein is not affected by the mode of expression, the use of strain-improvement procedures or the changes in glycosylation due to the fungal system.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The iron-dependent alcohol dehydrogenase from Zymomonas mobilis has been crystallized in a form suitable for X-ray diffraction studies. The crystals grew in hanging drops by vapor diffusion, equilibrating with a solution comprising 25–27% methoxypolyethylene glycol 5000 and 1 mM Co2+ in a 0.2 M succinic acid/potassium hydroxide buffer at pH 5.5–5.7 at 281 K. Crystals are tetragonal, P4122 (or P4322), with unit-cell dimensions a = b = 125.7, c = 248.1 Å. Four molecules comprise the asymmetric unit, and a self-rotation function indicates twofold local symmetry perpendicular to the unique axis and 15° from a crystallographic twofold axis. Diffraction data to 3.0 Å have been collected.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2023-06-27
    Keywords: 16 km ENE Cape Roberts; Cape Roberts Project; Comment; Core wireline system; CRP; CRP-1; CWS; DEPTH, sediment/rock; off Cape Roberts, Ross Sea, Antarctica; Sampling/drilling ice; δ13C, carbonate; δ18O, carbonate; δ18O, water
    Type: Dataset
    Format: text/tab-separated-values, 28 data points
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  • 4
    Publication Date: 2023-06-27
    Keywords: 16 km ENE Cape Roberts; Calcareous fossils; Calcium carbonate; Cape Roberts Project; Chert; Clay minerals; Core wireline system; CRP; CRP-1; CWS; DEPTH, sediment/rock; Heavy minerals; Intrusive, igneous rock; Iron carbonate, siderite; Kalifeldspar; Metamorphite; Mica; off Cape Roberts, Ross Sea, Antarctica; Opaque minerals; Plagioclase; Point counting with SEM/EDAX; Porosity; Pyroxene; Quartz; Sampling/drilling ice; Sedimentary rock; Sulfate; Volcanic glass; Volcanite
    Type: Dataset
    Format: text/tab-separated-values, 171 data points
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  • 5
    Publication Date: 2023-06-27
    Keywords: 16 km ENE Cape Roberts; Calcium carbonate; Cape Roberts Project; Comment; Core wireline system; CRP; CRP-1; CWS; DEPTH, sediment/rock; Energy dispersive X-ray analysis, EDAX; Iron carbonate, siderite; Magnesium carbonate, magnesite; Manganese carbonate, rhodochrosite; off Cape Roberts, Ross Sea, Antarctica; Sampling/drilling ice; Strontium carbonate, strontianite
    Type: Dataset
    Format: text/tab-separated-values, 120 data points
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  • 6
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    Unknown
    PANGAEA
    In:  Supplement to: Fielding, Christopher R; Baker, Julian C; Woolfe, Ken; Howe, John; Lavelle, Mark (1998): Reassessment of the Miocene-Quaternary boundary in CRP-1, Cape Roberts Project, Antarctica. Terra Antartica, 5(3), 425-426, hdl:10013/epic.28334.d001
    Publication Date: 2023-07-10
    Description: During the course of the 1997 drilling campaign, lithostratigraphic boundaries were assigned to the CRP-l core on the basis of perceived changes in lithology. The geologically most important boundary in the core, between the Miocene and overlying Quaternary sections, was placed at 43.55 mbsf. This horizon was described in the core logs (Cape Roberts Science Team, 1998) as a contact between muddy, finegrained sandstone (which were assigned a Lower Miocene age based on diatom biostratigsaphy) and overlying diamictons containing Quaternary diatoms. This boundary is a major unconformity, recognisable on seismic reflection records. As such, it has considerable significance in the ongoing scientific analysis of the drillcore. During a re-examination of the core, focusing on the archive half held at the Antarctic Geology Research Facility of the Florida State University at Tallahassee; the authors noted that the core across the published boundary (43.55 mbsf) did not show any lithological change, but logged a sharp contact between dark olive grey, muddy sandstone and overlying diamicton at 43.15 mbsf (Fig. l). We suggest, therefore, that the core log in appendix l of Cape Roberts Science Team (1998) is misleading over this interval. In order to test the veracity of the suggested boundary revision, a series of thin-sections was examined and point-counted for framework grain abundances.
    Keywords: 16 km ENE Cape Roberts; Calcite; Cape Roberts Project; Chert; Claystone; Core wireline system; CRP; CRP-1; CWS; DEPTH, sediment/rock; Epoch; Grain size, mean radius; Kalifeldspar; Micrite; off Cape Roberts, Ross Sea, Antarctica; Opaque minerals; Plagioclase; Porosity; Pyroxene; Quartz; Sampling/drilling ice; Sorting description; Thin section analysis/measurements; Volcanic glass; Volcanite
    Type: Dataset
    Format: text/tab-separated-values, 75 data points
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  • 7
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    PANGAEA
    In:  EPIC3Terra Antartica, Bremerhaven, PANGAEA, 5(3), pp. 425-426
    Publication Date: 2019-07-17
    Repository Name: EPIC Alfred Wegener Institut
    Type: PANGAEA Documentation , notRev
    Format: application/pdf
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  • 8
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    PANGAEA
    In:  EPIC3Terra Antartica, Bremerhaven, PANGAEA, 5(3), pp. 647-653
    Publication Date: 2019-07-17
    Repository Name: EPIC Alfred Wegener Institut
    Type: PANGAEA Documentation , notRev
    Format: application/pdf
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  • 9
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The NAD(P)-dependent glutamate dehydrogenase from Pyrococcus furiosus has been crystallized by the hanging-drop method of vapour diffusion using lithium sulfate as the precipitant. The crystals belong to the tetragonal system and are in space group P42212 with unit-cell dimensions of a = b = 167.2, c = 172.9 Å. Consideration of the values of Vm and possible packing of the molecules within the cell suggest that the asymmetric unit contains a trimer. P. furiosus belongs to the family of Archaea and is one of the most thermostable organisms known, having an optimal growth temperature of 376 K. The glutamate dehydrogenase isolated from this organism has a half-life of 12 h at 373 K and, therefore, the determination of the structure of this enzyme will be important in advancing our understanding of how proteins are adapted to enable them to survive at such extreme temperatures.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The structure of cytochrome c′ from two bacterial species, Alcaligenes sp and Alcaligenes denitrificans, have been determined from X-ray diffraction data to 3.0 Å resolution using the anomalous scattering of the single Fe atom in each to identify and refine a weak molecular-replacement solution. Molecular-replacement studies, with the program AMORE, used two isomorphous data sets (from the two species), two independent search models (the cytochromes c′ from Rhodospirillum molischianum and Rhodospirillum rubrum), both with and without side chains, and two different resolution ranges (10.0–4.0 and 15.0–3.5Å) to generate a large number of potential solutions. No single solution stood out and none appeared consistently. The Fe-atom position in each structure was then determined from its anomalous-scattering contribution and all molecular- replacement solutions were discarded which did not (i) place the Fe atom correctly and (ii) orient the molecule such that a crystallographic twofold axis generated a dimer like those of the two search models. Finally, electron-density maps phased solely by the Fe-atom anomalous scattering were calculated. As these were combined and subjected to solvent flattening and histogram matching (with the program SQUASH), correlation with the remaining molecular-replacement solutions identified one as correct and enabled it to be improved and subjected to preliminary refinement. The correctness of the solution is confirmed by parallel isomorphous-replacement studies.
    Type of Medium: Electronic Resource
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