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  • Wiley  (228)
  • Institute of Physics  (164)
  • Wiley-Blackwell  (48)
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  • 1
    Electronic Resource
    Electronic Resource
    Distribution of acetylated α-tubulin in retina and in in vitro - assembled microtubules (1988)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 9 (1988), S. 243-253 
    Details
    ISSN: 0886-1544
    Keywords: neurons ; posttranslational modification ; tubulin isoforms ; rod and cone photoreceptors ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We have used the mouse monoclonal antibody 6-11B-1, specific for acetylated α-tubulin, to determine the distribution of acetylated α-tubulin in in vitro-assembled microtubules and retinal tissue. Analysis by immunoblots revealed that microtubules assembled from bovine brain extracts contain both acetylated and nonacetylated α-tubulin. Immunofluorescence, using 6-11B-1 and antitubulin B-5-1-2, a monoclonal antibody specific for α-tubulin, demonstrated the colocalization of both α-tubulin species in neurons of the retina and that acetylated microtubules are relatively abundant in neurons. However, analysis at higher resolution revealed that rod photoreceptors contain spatially distinct microtubule arrays which differ in content of acetylated α-tubulin and differ in stability. Acetylated microtubules which composed those of the rod outer segment and connecting cilium were resistant to depolymerization in nocodazole or colchicine. In contrast, the nonacetylated microtubules which composed those of the rod-inner segment were depolymerized in nocodazole or colchicine. Therefore, these acetylated microtubules are more resistant to depolymerization than non-acetylated microtubules.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970090306
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  • 2
    Electronic Resource
    Electronic Resource
    Transmembrane assemblage of the photoreceptor connecting cilium and motile cilium transition zone contain a common immunologic epitope (1990)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 17 (1990), S. 329-344 
    Details
    ISSN: 0886-1544
    Keywords: cytoskeleton ; glycoconjugates ; axoneme ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The photoreceptor connecting cilium bears a unique transmembrane assemblage which stably links cell surface glycoconjugates with the underlying axonemal cytoskeleton. Structural similarities between the photoreceptor connecting cilium and the transition zone of motile cilia suggests that this assemblage may also be present in motile cilia. Using a subcellular fraction enriched in detergent-extracted photoreceptor axonemes, three high molecular mass glycoconjugates (425, 600, and 700 kD) were previously identified as potential components of the assemblage. Through oligosaccharide characterization and binding of a specific monoclonal antibody, we have verified the localization of the 425 kD glycoconjugate to the transmembrane assemblage. Binding of the lectin peanut agglutinin (PNA) to the 425 kD glycoconjugate on nitrocellulose blots, and to isolated detergentextracted axonemes, was assessed following treatment with the enzymes neuraminidase and O-glycanase. Changes in binding to the 425 kD glycoconjugate precisely paralleled changes in binding to intact axonemes, supporting the hypothesis that the 425 kD glycoconjugate is a component of the transmembrane assemblage. Furthermore, the results suggest that the 425 kD glycoconjugate contains sialated galactose-N-acetylgalactosamine oligosaccharides which are Olinked to the protein backbone. To directly assess the distribution of the 425 kD glycoconjugate, we produced a monoclonal antibody directed against this glycoconjugate. The antibody, K26, recognizes only the 425 kD on transblots of the axoneme fraction. K26 immunoreactivity of intact axonemes is identical to that seen by PNA staining. K26 staining of isolated photoreceptors and whole retina is uniquely localized to the region of the connecting cilium. Thus, in the photoreceptor, the 425 kD is not only a component of the transmembrane assemblage but is also completely restricted to the connecting cilium.Based on morphological similarities, the photoreceptor connecting cilium is thought to be homologous to the transition zone of the motile cilium. As such, we have stained oviduct epithelium with the K26 monoclonal antibody. Immunoreactivity is restricted to the region of the transition zone at the base of motile cilia. This demonstrates that the photoreceptor connecting cilium and motile cilium transition zone are immunologically related.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970170408
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  • 3
    Electronic Resource
    Electronic Resource
    Cytoskeletal alterations in cultured cardiomyocytes following exposure to the lipid peroxidation product, 4-hydroxynonenal (1994)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 28 (1994), S. 119-134 
    Details
    ISSN: 0886-1544
    Keywords: microtubules ; vinculin ; desmin ; sarcolemmal damage ; free radicals ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Damage to the cardiac myocyte sarcolemma following any of several pathological insults such as ischemia (anoxia) alone or followed by reperfusion (reoxygenation), is most apparent as progressive sarcolemmal blebbing, an event attributed by many investigators to a disruption in the underlying cytoskeletal scaffolding. Scanning electron microscopic observation of tissue cultured rat neonatal cardiomyocytes indicates that exposure of these cells to the toxic aldehyde 4-hydroxynonenal (4-HNE), a free radical--induced, lipid peroxidation product, results in the appearance of sarcolemmal blebs, whose ultimate rupture leads to cell death. Indirect immunofluorescent localization of a number of cytoskeletal components following exposure to 4-HNE reveals damage to several, but not all, key cytoskeletal elements, most notably microtubules, vinculin-containing costameres, and intermediate filaments. The exact mechanism underlying the selective disruption of these proteins cannot be ascertained at this time. Colocalization of actin indicated that whereas elements of the cytoskeleton were disrupted by increasing length of exposure to 4-HNE, neither the striated appearance of the myofibrils nor the lateral register of neighboring myofibrils was altered. Monitoring systolic and diastolic levels of intracellular calcium ([Ca2+]i) indicated that increases in [Ca2+]i occurred after considerable cytoskeletal changes had already taken place, suggesting that damage to the cytoskeleton, at least in early phases of exposure to 4-HNE, does not involve Ca2+ -dependent proteases. However, 4-HNE-induced cytoskeletal alterations coincide with the appearance of, and therefore suggest linkage to, sarcolemmal blebs in cardiac myocytes.Although free radicals produced by reperfusion or reoxygenation of ischemic tissue have been implicated in cellular damage, these studies represent the first evidence linking cardiomyocyte sarcolemmal damage to cytoskeletal disruption produced by a free radical product. © 1994 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970280204
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  • 4
    Electronic Resource
    Electronic Resource
    Complex intermolecular interactions maintain a stable linkage between the photoreceptor connecting cilium axoneme and plasma membrane (1994)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 28 (1994), S. 213-230 
    Details
    ISSN: 0886-1544
    Keywords: axoneme-plasmalemma cross-linkers ; cytoskeleton-linked glycoconjugates ; cytoskeleton-membrane interactions ; hydrophobic interactions ; lectin cytochemistry ; SDS-resistance ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Microtubule-membrane cross-linkers in motile and nonmotile cilia are supramolecular structures, held together by strong interactions between the constituent molecules. We have characterized these interactions in the photoreceptor connecting cilium, where cross-linkers co-fractionate and maintain their in situ location after Triton X-100 extraction of axonemes. In bovine photoreceptor cells, the transmembrane assemblage that is cross-linked to the connecting cilium axoneme contains three high molecular mass glycoconjugates of 425, 600, and 700 kDa (Horst et al., 1987). The relative amounts of the three glycoconjugates, as judged from band intensity in electrophoretograms, depend strongly on sample treatment prior to electrophoresis. The electrophoretic pattern was reproducible after several weeks of storage of the axoneme fraction in extraction buffer containing 50% sucrose. Removal of sucrose from the buffer by dialysis eliminated the 600 kDa and 700 kDa, and decreased the detected amount of the 425 kDa glycoconjugate. When samples were incubated in Laemmli sample buffer at increasing temperatures (23°, 60°, 95°C), a gradual reduction in the intensity of the three bands was observed. The quantitative reduction of high molecular mass glycoconjugates was accompanied by the appearance of novel protein species of lower molecular mass, as detected by lectin and antibody overlays of axonemal transblots. These results suggest that the previously characterized cross-linker glycoconjugates are complex, SDS-resistant multi-molecular conglomerates. We have further used fluorescent lectins to monitor the presence of glycoconjugates on whole-mounted axonemes, in conditions aimed to selectively solubilize the cross-linkers. The cross-linker complexes could not be dissociated from the axoneme by incubation with buffers containing 1 M of either Na2SO4 or NaI. The results indicate that the connecting cilium-specific cross-linker complexes are bound via high-affinity interactions to both axoneme and overlying plasma membrane. © 1994 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970280305
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  • 5
    Electronic Resource
    Electronic Resource
    Separation of amniotic fluid phospholipids by one-dimensional TLC (1979)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 2 (1979), S. 199-200 
    Details
    ISSN: 0935-6304
    Keywords: Thin-layer chromatography, TLC ; Phosolipid separation, L/S ratio determination ; Quantitation above 100 ng, optimized ; Cupric acetate-phosphoric reagent superior to 3 other reagents ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240020413
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  • 6
    Electronic Resource
    Electronic Resource
    Bimolecular reactions of ·CH2CH2OCH2+: A comparison with the chemical properties of CH3CH2OCH2+ and ionized trimethylene oxide (1992)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 6 (1992), S. 734-740 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: The chemical properties of the distonic radical cation ·CH2CH2OCH2+ are compared with those of its conventional isomer, ionized trimethylene oxide, and its even-electron analog, the ethoxymethyl cation (CH3CH2OCH2+). The rate constants and branching rations have been determined for the gas-phase reactions of the three ions with several neutral reagents in a dual-cell Fourier-transform ion cyclotron resonance device. The reactivity of each ion is found to be quite distinct. Fast electron transfer dominates most of the bimolecular reactions of ionized trimethyleene oxide. The ethoxymethyl cation reacts slowly or not at all with the reagents studied, and the only reaction observed is ethyl cation transfer. The distonic ion ·CH2CH2OCH2+ undergoes vedry fast reactions with all the neutral reagents, and, in sharp contrast to the other two ions, shows remarkably versatile reactivity. This ion rapidly transfers ionized ethylene to most of the neutral molecules studied. Facile electron transfer occurs with some of the neutral reagents while others abstract a pproton from the ion. Abstraction of a radical by the distonic ion-a reaction reported earlier for dimethyl disulfide - is not observed for any other reagent.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290061205
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  • 7
    Electronic Resource
    Electronic Resource
    Complexes of tricoordinate hypervalent pnictogens with pentamethylcyclopentadienylrutheniumContribution No. 4422 (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Heteroatom Chemistry 1 (1990), S. 407-417 
    Details
    ISSN: 1042-7163
    Keywords: Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The complexes of a pentamethylcyclopentadienylruthenium moiety with hypervalent tricoordinate pnictogens are reported. A unique mode of complexation is observed for each of the different pnictogens (P, As, Sb). The phosphorus derived complex exhibits an 8-electron tetrahedral bonding environment at phosphorus. The antimony derived complex maintains a 10-electron bonding system at antimony with a pseudo-trigonalbipyramidal geometry at antimony. The arsenic-containing complex is formed with destruction of the original arsenic heterocycle and formation of a trinuclear Ru-Ru-As ring. Remarkably, the formation of the arsenic ruthenium complex can be reversed to reconstruct the original arsenic heterocycle.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hc.520010509
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  • 8
    Electronic Resource
    Electronic Resource
    The syntheses and structures of iron carbonyl adducts of tricoordinated hypervalent phosphorus compoundsDedicated to Professor Dr. Marianne Baudler on the occasion of her seventieth birthday. (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Heteroatom Chemistry 2 (1991), S. 395-407 
    Details
    ISSN: 1042-7163
    Keywords: Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The hypervalent phosphorus compound 3,7-di-t-butyl-5-aza-2,8-dioxa-1-phosphabicyclo[3.3.0]octa-2,4,6-triene(ADPO), forms a monosubstituted adduct, ADPO·Fe(CO)4, by direct reaction of 10-P-3 ADPO with Fe2(CO)9 or Fe(CO)5, as well as by reaction of 1,1-dichloro-3,7-di-t-butyl-5-aza-2,8-dioxa-1-phosphabicyclo[3.3.0]octa-3, 6-diene(ADPO·Cl2) with Na2[Fe(CO)4]. The X-ray crystal structure of ADPO·Fe(CO)4 shows that ADPO is coordinated to the iron through the phosphorus. The phosphorus of the adduct has a tetrahedral 8-P-4 geometry in contrast to the planar T-shaped geometry of uncomplexed 10-P-3 ADPO. Ultraviolet photolysis of ADPO·Fe(CO)4 yields the disubstituted species (ADPO)2·e(CO)3 wherein ADPO has dimerized via P-O bond cleavage to form a bidentate (ADPO)2 ligand containing a 10-membered ring that bridges axial and equatorial positions at the trigonal bipyramidal iron center.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hc.520020307
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  • 9
    Electronic Resource
    Electronic Resource
    Effects of continuous light and darkness on the eyes of the troglobitic salamander Typhlotriton spelaeusThis paper is based in part on a dissertation submitted by the senior author in partial fulfillment of requirements for a Ph.D. degree at Southern Dlinois University at Carbondale. (1976)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 149 (1976), S. 527-545 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Larval Typhlotriton spelaeus collected from five caves in Pulaski Co., Missouri, were kept as larvae or induced to transform in darkness or continuous fluorescent illumination. Larvae maintained in darkness for 215 and 279 days had smaller eyes, smaller rod inner and outer segments, and fewer metaphase figures in the genninative zone of the neural retina than comparable larvae maintained in light (258 lux). Except for visual cell size, differences were small and for each characteristic exceptions were observed. One larva kept in light showed early retinal degeneration comparable to that in transformed adults of T. spelaeus. All larvae exhibited optomotor behavior both before and after the experiment.Among animals induced to transform by L-thyroxin and maintained in darkness 111 to 366 days, visual cell and pigment epithelium degeneration was more extensive and more frequent than in animals kept for the same length of time in light (237-298 lux). In darkness the frequency of animals with retinal degeneration increased between 111 and 366 days. In light some animals exhibited pigment epithelium reduction with normal visual cells, and others had free, pigmented cells in the subretinal space. These effects were not comparable to degeneration in darkness. Eyelids covered the eyes of only a few animals in both light and dark treatments. The extent of eyelid encroachment over the eye was greater in darkness than in light. Most animals exhibited optomotor responses after experiments, but responses of animals kept in darkness were impaired in comparison to those of animals kept in light.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051490406
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  • 10
    Electronic Resource
    Electronic Resource
    Venting of runaway reactions with gas generation (1992)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 38 (1992), S. 723-732 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Pressure relief venting of a runway reaction with gas generation is examined in terms of the overall system response and the associated venting requirement. This article presents an exact formulation as well as an approximate analytical approach. The latter is shown to be particularly useful in vent-sizing applications. Using an aqueous hydrogen peroxide decomposition example, the analytical results are demonstrated to agree well with the exact numerical results over a wide range of overpressure. In addition, the analytical result is shown to reduce to the correct limit for a pure vapor system and offers a useful vent-sizing equation for a pure-gassy system.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690380509
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