ALBERT

Description: The Southern California Bight is adjacent to one of the world's largest urban areas, Los Angeles. As a consequence, anthropogenic impacts could disrupt local marine ecosystems due to municipal and industrial waste discharge, pollution, flood control measures, and global warming. Santa Monica Basin (SMB), due to its unique setting in a low-oxygen and high-sedimentation environment, can provide an excellent sedimentary paleorecord of these anthropogenic changes. This study examined 10 sediment cores, collected from different parts of the SMB between spring and summer 2016, and compared them to existing cores in order to document changes in sedimentary dynamics during the last 250 years, with an emphasis on the last 40 years. The 210Pb-based mass accumulation rates (MARs) for the deepest and lowest oxygen-containing parts of the SMB basin (900–910 m) have been remarkably consistent during the past century, averaging 17.1±0.6 mg cm−2 yr−1. At slightly shallower sites (870–900 m), accumulation rates showed more variation but yield the same accumulation rate, 17.9±1.9 mg cm−2 yr−1. Excess 210Pb sedimentation rates were consistent with rates established using bomb test 137Cs profiles. We also examined 14C profiles from two cores collected in the deepest part of the SMB, where fine laminations are present up to about 450 yr BP. These data indicate that the MAR was slower prior to ∼1900 CE (rates obtained were 9 and 12 mg cm−2 yr−1). The δ13Corg profiles show a relatively constant value where laminations are present, suggesting that the change in sediment accumulation rate is not accompanied by a change in organic carbon sources to the basin. The increase in sedimentation rate towards the Recent occurs at about the time previous studies predicted an increase in siltation and the demise of a shelly shelf benthic fauna on the SMB shelf. X-radiographs show finely laminated sediments in the deepest part of the basin only, with centimeter-scale layering of sediments or no layering whatsoever in shallower parts of the SMB basin. The absence of finely laminated sediments in cores MUC 10 (893 m) and MUC 3 (777 m) suggests that the rate at which anoxia is spreading has not increased appreciably since cores were last analyzed in the 1980s. Based on core top data collected during the past half century, sedimentary dynamics within SMB have changed minimally during the last 40 years. Specifically, mass accumulation rates, laminated sediment fabric, extent of bioturbation and % Corg have not changed. The only parameter that appeared to have changed in the last 450 years was the MAR, with an apparent 〉 50 % increase occurring between ∼1850 CE and the early 1900s. The post-1900 CE constancy of sedimentation through a period of massive urbanization in Los Angeles is surprising.

Description: Coastal seas may account for more than 75 % of global oceanic methane emissions. There, methane is mainly produced microbially in anoxic sediments from which it can escape to the overlying water column. Aerobic methane oxidation (MOx) in the water column acts as a biological filter, reducing the amount of methane that eventually evades to the atmosphere. The efficiency of the MOx filter is potentially controlled by the availability of dissolved methane and oxygen, as well as temperature, salinity, and hydrographic dynamics, and all of these factors undergo strong temporal fluctuations in coastal ecosystems. In order to elucidate the key environmental controls, specifically the effect of oxygen availability, on MOx in a seasonally stratified and hypoxic coastal marine setting, we conducted a 2-year time-series study with measurements of MOx and physico-chemical water column parameters in a coastal inlet in the south-western Baltic Sea (Eckernförde Bay). We found that MOx rates generally increased toward the seafloor, but were not directly linked to methane concentrations. MOx exhibited a strong seasonal variability, with maximum rates (up to 11.6 nmol L−1 d−1) during summer stratification when oxygen concentrations were lowest and bottom-water temperatures were highest. Under these conditions, 2.4–19.0 times more methane was oxidized than emitted to the atmosphere, whereas about the same amount was consumed and emitted during the mixed and oxygenated periods. Laboratory experiments with manipulated oxygen concentrations in the range of 0.2–220 µmol L−1 revealed a submicromolar oxygen optimum for MOx at the study site. In contrast, the fraction of methane–carbon incorporation into the bacterial biomass (compared to the total amount of oxidized methane) was up to 38-fold higher at saturated oxygen concentrations, suggesting a different partitioning of catabolic and anabolic processes under oxygen-replete and oxygen-starved conditions, respectively. Our results underscore the importance of MOx in mitigating methane emission from coastal waters and indicate an organism-level adaptation of the water column methanotrophs to hypoxic conditions.

Description: The presence of surface methanogenesis, located within the sulfate-reducing zone (0–30 centimeters below seafloor, cmbsf), was investigated in sediments of the seasonally hypoxic Eckernförde Bay, southwestern Baltic Sea. Water column parameters like oxygen, temperature and salinity together with porewater geochemistry and benthic methanogenesis rates were determined in the sampling area ''Boknis Eck'' quarterly from March 2013 to September 2014, to investigate the effect of seasonal environmental changes on the rate and distribution of surface methanogenesis and to estimate its potential contribution to benthic methane emissions. The metabolic pathway of methanogenesis in the presence or absence of sulfate reducers and after the addition of a non-competitive substrate was studied in four experimental setups: 1) unaltered sediment batch incubations (net methanogenesis), 2) 14C-bicarbonate labeling experiments (hydrogenotrophic methanogenesis), 3) manipulated experiments with addition of either molybdate (sulfate reducer inhibitor), 2-bromoethane-sulfonate (methanogen inhibitor), or methanol (non-competitive substrate, potential methanogenesis), 4) addition of 13C-labeled methanol (potential methylotrophic methanogenesis). After incubation with methanol in the manipulated experiments, molecular analyses were conducted to identify key functional methanogenic groups. Hydrogenotrophic methanogenesis in sediments below the sulfate-reducing zone (〉 30 cmbsf) was determined by 14C-bicarbonate radiotracer incubation in samples collected in September 2013. Surface methanogenesis changed seasonally in the upper 30 cmbsf with rates increasing from March (0.2 nmol cm−3 d−1) to November (1.3 nmol cm−3 d−1) 2013 and March (0.2 nmol cm−3 d−1) to September (0.4 nmol cm−3 d−1) 2014, respectively. Its magnitude and distribution appeared to be controlled by organic matter availability, C / N, temperature, and oxygen in the water column, revealing higher rates in warm, stratified, hypoxic seasons (September/November) compared to colder, oxygenated seasons (March/June) of each year. The majority of surface methanogenesis was likely driven by the usage of non-competitive substrates (e.g., methanol and methylated compounds), to avoid competition with sulfate reducers, as it was indicated by the 1000–3000-fold increase in potential methanogenesis activity observed after methanol addition. Accordingly, competitive hydrogenotrophic methanogenesis increased in the sediment only below the depth of sulfate penetration (〉 30 cmbsf). Members of the family Methanosarcinaceae, which are known for methylotrophic methanogenesis, were detected by PCR using Methanosarcinaceae-specific primers and are likely to be responsible for the observed surface methanogenesis. The present study indicated that surface methanogenesis makes an important contribute to the benthic methane budget of Eckernförde Bay sediments as it could directly feed into methane oxidation above the sulfate-methane transition zone.

Description: Coastal seas may account for more than 75 % of global oceanic methane emissions. There, methane is mainly produced microbially in anoxic sediments from where it can escape to the overlying water column. Aerobic methane oxidation (MOx) in the water column acts as a biological filter reducing the amount of methane that eventually evades to the atmosphere. The efficiency of the MOx filter is potentially controlled by the availability of dissolved methane and oxygen, as well as temperature, salinity, and hydrographic dynamics, and all of these factors undergo strong temporal fluctuations in coastal ecosystems. In order to elucidate the key environmental controls, specifically the effect of oxygen availability, on MOx in a seasonally stratified and hypoxic coastal marine setting, we conducted a 2-year time-series study with measurements of MOx and physico-chemical water column parameters in a coastal inlet in the southwestern Baltic Sea (Eckernförde Bay). We found that MOx rates always increased toward the seafloor, but were not directly linked to methane concentrations. MOx exhibited a strong seasonal variability, with maximum rates (up to 11.6 nmol l−1 d−1) during summer stratification when oxygen concentrations were lowest and bottom-water temperatures were highest. Under these conditions, 70–95 % of the sediment-released methane was oxidized, whereas only 40–60 % were consumed during the mixed and oxygenated periods. Laboratory experiments with manipulated oxygen concentrations in the range of 0.2–220 µmol l−1 revealed a sub-micromolar oxygen-optimum for MOx at the study site. In contrast, the fraction of methane-carbon incorporation into the bacterial biomass (compared to the total amount of oxidised methane) was up to 38-fold higher at saturated oxygen concentrations, suggesting a different partitioning of catabolic and anabolic processes under oxygen-replete and oxygen-starved conditions, respectively. Our results underscore the importance of MOx in mitigating methane emission from coastal waters and indicate an organism-level adaptation of the water column methanotrophs to hypoxic conditions.

Description: The potential coupling of nitrogen (N2) fixation and sulfate reduction (SR) was explored in sediments of the Peruvian oxygen minimum zone (OMZ). Sediment samples were retrieved by a multiple corer at six stations along a depth transect (70–1025 m water depth) at 12° S, covering anoxic and hypoxic bottom water conditions. Benthic N2 fixation, determined by the acetylene reduction assay, was detected at all sites, with highest rates between 70 and 253 m and lower rates at greater depth. SR rates decreased with increasing water depth. N2 fixation and SR overlapped in sediments, suggesting a potential coupling of both processes. However, a weak positive correlation of their activity distribution was detected by principle component analysis. A potential link between N2 fixation and sulfate-reducing bacteria was indicated by the molecular analysis of nifH genes. Detected nifH sequences clustered with the sulfate-reducing bacteria Desulfonema limicola at the 253 m station. However, nifH sequences of other stations clustered with uncultured organisms, Gammaproteobacteria, and Firmicutes (Clostridia) rather than with known sulfate reducers. The principle component analysis revealed that benthic N2 fixation in the Peruvian OMZ is controlled by organic matter (positive) and free sulfide (negative). No correlation was found between N2 fixation and ammonium concentrations (even at levels 〉 2022 µM). N2 fixation rates in the Peruvian OMZ sediments were in the same range as those measured in other organic-rich sediments.

Description: Benthic microbial methanogenesis is a known source of methane in marine systems. In most sediments, the majority of methanogenesis is located below the sulfate-reducing zone, as sulfate reducers outcompete methanogens for the major substrates hydrogen and acetate. The coexistence of methanogenesis and sulfate reduction has been shown before and is possible through the usage of noncompetitive substrates by methanogens such as methanol or methylated amines. However, knowledge about the magnitude, seasonality, and environmental controls of this noncompetitive methane production is sparse. In the present study, the presence of methanogenesis within the sulfate reduction zone (SRZ methanogenesis) was investigated in sediments (0–30 cm below seafloor, cm b.s.f.) of the seasonally hypoxic Eckernförde Bay in the southwestern Baltic Sea. Water column parameters such as oxygen, temperature, and salinity together with porewater geochemistry and benthic methanogenesis rates were determined in the sampling area Boknis Eck quarterly from March 2013 to September 2014 to investigate the effect of seasonal environmental changes on the rate and distribution of SRZ methanogenesis, to estimate its potential contribution to benthic methane emissions, and to identify the potential methanogenic groups responsible for SRZ methanogenesis. The metabolic pathway of methanogenesis in the presence or absence of sulfate reducers, which after the addition of a noncompetitive substrate was studied in four experimental setups: (1) unaltered sediment batch incubations (net methanogenesis), (2) 14C-bicarbonate labeling experiments (hydrogenotrophic methanogenesis), (3) manipulated experiments with the addition of either molybdate (sulfate reducer inhibitor), 2-bromoethanesulfonate (methanogen inhibitor), or methanol (noncompetitive substrate, potential methanogenesis), and (4) the addition of 13C-labeled methanol (potential methylotrophic methanogenesis). After incubation with methanol, molecular analyses were conducted to identify key functional methanogenic groups during methylotrophic methanogenesis. To also compare the magnitudes of SRZ methanogenesis with methanogenesis below the sulfate reduction zone (〉 30 cm b.s.f.), hydrogenotrophic methanogenesis was determined by 14C-bicarbonate radiotracer incubation in samples collected in September 2013.SRZ methanogenesis changed seasonally in the upper 30 cm b.s.f. with rates increasing from March (0.2 nmol cm−3 d−1) to November (1.3 nmol cm−3 d−1) 2013 and March (0.2 nmol cm−3 d−1) to September (0.4 nmol cm−3 d−1) 2014. Its magnitude and distribution appeared to be controlled by organic matter availability, C / N, temperature, and oxygen in the water column, revealing higher rates in the warm, stratified, hypoxic seasons (September–November) compared to the colder, oxygenated seasons (March–June) of each year. The majority of SRZ methanogenesis was likely driven by the usage of noncompetitive substrates (e.g., methanol and methylated compounds) to avoid competition with sulfate reducers, as was indicated by the 1000–3000-fold increase in potential methanogenesis activity observed after methanol addition. Accordingly, competitive hydrogenotrophic methanogenesis increased in the sediment only below the depth of sulfate penetration (〉 30 cm b.s.f.). Members of the family Methanosarcinaceae, which are known for methylotrophic methanogenesis, were detected by PCR using Methanosarcinaceae-specific primers and are likely to be responsible for the observed SRZ methanogenesis.The present study indicates that SRZ methanogenesis is an important component of the benthic methane budget and carbon cycling in Eckernförde Bay. Although its contributions to methane emissions from the sediment into the water column are probably minor, SRZ methanogenesis could directly feed into methane oxidation above the sulfate–methane transition zone.

Description: In the current era of rapid climate change, accurate characterization of climate-relevant gas dynamics – namely production, consumption, and net emissions – is required for all biomes, especially those ecosystems most susceptible to the impact of change. Marine environments include regions that act as net sources or sinks for numerous climate-active trace gases including methane (CH4) and nitrous oxide (N2O). The temporal and spatial distributions of CH4 and N2O are controlled by the interaction of complex biogeochemical and physical processes. To evaluate and quantify how these mechanisms affect marine CH4 and N2O cycling requires a combination of traditional scientific disciplines including oceanography, microbiology, and numerical modeling. Fundamental to these efforts is ensuring that the datasets produced by independent scientists are comparable and interoperable. Equally critical is transparent communication within the research community about the technical improvements required to increase our collective understanding of marine CH4 and N2O. A workshop sponsored by Ocean Carbon and Biogeochemistry (OCB) was organized to enhance dialogue and collaborations pertaining to marine CH4 and N2O. Here, we summarize the outcomes from the workshop to describe the challenges and opportunities for near-future CH4 and N2O research in the marine environment.