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  • 1
    Electronic Resource
    Electronic Resource
    Photofission with linearly polarized photons (1982)
    Springer
    The European physical journal 308 (1982), S. 63-71 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The formalism for fragment angular distributions in photofission with unpolarized and linearly polarized photons is summarized. First experiments on232Th using linearly polarized bremsstrahlung are described. The results for the analyzing power show for the first time in a model independent way that the low energy photofission can be explained byelectric dipole excitation. The possibilities of this new method are discussed, in particular when using monoenergetic tagged photons, which are available soon at new high duty cycle electron accelerators.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01415850
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  • 2
    Electronic Resource
    Electronic Resource
    Electrophoretic techniques for detection of Glypta fumiferanae, an endoparasitoid of western spruce budworm (1981)
    Springer
    Entomologia experimentalis et applicata 30 (1981), S. 176-180 
    Details
    ISSN: 1570-7458
    Keywords: Western Spruce Budworm ; Choristoneura occidentalis ; Glypta fumiferanae ; endoparasitoids ; electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Raupen von Choristoneura occidentalis ohne und mit dem Endoparasitoiden Glypta fumiferanae (Vier.) (Hymenoptera: Ichneumonidae) wurden elektrophoretisch auf Isoenzymbänder untersucht zum Nachweis des Parasitoiden. Für G. fumiferanae typische Bänder wurden in 4 der untersuchten 15 Enzymen beobachtet. Dies waren Aconitase, Esterase, Leucinaminopeptidase und 6-Phosphoglucose-dehydrogenase. Das Vorkommen dieser Isoenzymbänder ist ein zuverlässiges Zeichen der Parasitierung durch G. fumiferanae. Die Brauchbarkeit der Elektrophorese als Mittel zum Routinenachweis von Parasitoiden wird diskutiert.
    Notes: Abstract Parasitoid-free western spruce budworms and western spruce budworm larvae containing the endoparasitoid Glypta fumiferanae (Vier.) (Hymenoptera: Ichneumonidae) were assayed electrophoretically to identify isozyme bands diagnostic for the parasitoid. Bands characteristic of G. fumiferanae were observed with 4 of 15 enzymes tested. These were aconitase, esterase, leucine aminopeptidase and 6-phosphoglucose dehydrogenase. Presence of these isozyme bands is a reliable indication of parasitism by G. fumiferanae. The efficiency of electrophoresis as a tool for routine detection of parasitoids is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300884
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  • 3
    Electronic Resource
    Electronic Resource
    Pore size and properties of spherical Ca-alginate biocatalysts (1983)
    Springer
    Applied microbiology and biotechnology 18 (1983), S. 86-91 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A method for the production of spherical alginate gels from few μm up to several mm diameter is described. Alginate beads with diameters from 0.5 to 3 mm are used to immobilize microorganisms as biocatalysts. Micro size alginate gels are used as column packing material for inverse steric exclusion chromatography to determine their pore sizes from dextran standards of known molecular weight. The results indicate that alginate gels have pores which are large enough to release catalytic acting enzymes out of the matrix.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00500829
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  • 4
    Electronic Resource
    Electronic Resource
    Chromosomal evolution in serpentes; a comparison of G and C chromosome banding patterns of some colubrid and boid genera (1980)
    Springer
    Chromosoma 79 (1980), S. 53-64 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract G and C-chromosome banding techniques have been used to compare the structure of the karyotype in a variety of colubrid and boid snakes. The comparison of G-band patterns indicates that while some band sequences have been conserved, either as whole chromosomes or entire arms, there is also evidence of considerable rearrangement especially in the smaller chromosomes. In the colubrid Elaphe subocularis there is also evidence that there has been a relocation of the centromere on chromosome 2 without any accompanying inversion in the sequence of G-bands. Finally, G-banding has facilitated the demonstration of a simple pericentric inversion distinguishing the Z and W chromosomes in Acrantophis dumereli. This represents the first report of differentiated sex chromosomes in a boid snake. The combined banding data thus indicates that snake chromosomes are certainly not lacking in variability. The use of C-banding to detect constitutive heterochromatin has confirmed that in some boids and colubrids macrochromosomes have been derived from microchromosomes by the additions of heterochromatin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00328472
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  • 5
    Electronic Resource
    Electronic Resource
    Copper accumulation in primary biliary cirrhosis (1982)
    Springer
    Histochemistry and cell biology 74 (1982), S. 85-93 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural and X-Ray microanalytical aspects of primary biliary cirrhosis were analysed and compared to those of normal liver. Electron microscopic studies showed a significant increase of dense lipofuscin-like granules in PBC compared to the hepatocytes of normal liver (665%). These granules (0.3–2.7 μm in diameter) were formed by the association of primary lysosomes and lipid droplets. X-Ray microanalysis of these structures showed the presence of Calcium, Phosphorus, Potassium, Chlorine, Sulphur, Aluminium and Copper. The coexistence of Sulphur with other mineral elements suggests the occurence of metalloproteins binding these elements. These findings provide evidence that intralysosomal sequestration of mineral elements, especially copper protects the hepatocytes from cytotoxic effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495054
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  • 6
    Electronic Resource
    Electronic Resource
    A new method to measure particle turbulent dispersion using laser doppler anemometer (1984)
    Springer
    Experiments in fluids 2 (1984), S. 89-93 
    Details
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A laser doppler anemometer (LDA) was used to measure local dispersion coefficients of particles in turbulent flow. The experimental set-up is described along with the data acquisition equipment and processing procedures. Results for 5 μ particles dispersing from a point source in pipe flow are shown. A second estimate for the diffusivity was obtained from mean square dispersion measurements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261327
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  • 7
    Electronic Resource
    Electronic Resource
    Rat major histocompatibility RT1.C antigens of restricted tissue distribution consist of two polypeptide chains with molecular weights of about 42 000 and 12 500 (1982)
    Springer
    Immunogenetics 15 (1982), S. 271-277 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The RT1.C region has recently been defined genetically as a third region of the rat major histocompatibility system, RT1, and has been shown to code for cell-surface antigens with restricted tissue distribution (present on lymphocytes and absent from red blood cells and liver) and for target antigens of unrestricted cytotoxic T lymphocytes. Immunoprecipitation with C-antigen-specific alioantisera and SDS-PAGE analysis, reveal that C-region products are glycoproteins composed of molecules of 40 500–43 000 and 12 500 molecular weight, respectively. Thus, the RT1.B region, which codes for class II molecules, is flanked by RT1 regions which determine class I molecules (as defined biochemically), which are either expressed ubiquitously (RT1.A antigens) or in a restricted manner (RT1.C products). The homology to H-2Qa antigens is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00364335
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  • 8
    Electronic Resource
    Electronic Resource
    Protein synthesis in liver, skeletal muscle, and brown adipose tissue of rats fed a protein-deficient diet (1983)
    Springer
    Bioscience reports 3 (1983), S. 569-575 
    Details
    ISSN: 1573-4935
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Feeding protein-deficient diets to rats is known to stimulate diet-induced thermogenesis and activate brown adipose tissue (BAT). The fact that BAT protein content, unlike that of other tissues, is unnaffected by protein deficiency prompted us to measure tissue protein synthesis in vivo in animals maintained on normal- (18.8%) and low- (7.6%) protein (LP) diets. Protein synthesis was depressed in the liver of the LP rats due to a fall in RNA activity, with no change in RNA content, and synthesis was also reduced in skeletal muscle from the LP group, but this was due to decreased RNA c o n t e n t with no change in RNA activity. Conversely, protein synthesis, RNA, DNA, and protein content of interscapular BAT were all unaltered in protein-restricted animals. These data indicate that, unlike liver, skeletal muscle, and whole carcass, BAT protein synthesis is not reduced in protein-restricted rats, and this may be related to activation of thermo-genesis in the tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01120702
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  • 9
    Electronic Resource
    Electronic Resource
    Identification of two mitochondrial GDP-binding sites in rat brown adipose tissue (1983)
    Springer
    Bioscience reports 3 (1983), S. 589-598 
    Details
    ISSN: 1573-4935
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Scatchard analysis of specific guanosine-diphosphate-([3H]GDP-) binding to rat brown-adipose-tissue mitochondria revealed two distinct binding sites with apparent dissociation constants (Kd) of approximately 0.05 and 2.0 μM. Binding to both sites was insensitive to atractyloside. Reducing the pH of the binding medium from 7.1 to 6.6 caused marked reductions in the Kd of both sites, but at pH 7.6, the dissociation constants were increased about 3-fold. Acute treatment of rats with noradrenaline, 1 h before sacrifice, increased the maximum number of binding sites (Bmax, pmol/rng mitochondrial protein) of both sites and also increased the dissociation constants. The Bmax of the lower-affinity site was elevated in rats exposed to 5°C or fed a palatable cafeteria diet for 10 d, compared to control animals, with the greater changes occurring in the cold-adapted group. The high-affinity site was unaltered by cold adaptation or cafeteria feeding. These results indicate the presence of two distinct nucleotide-binding sites in brown-fat mitochondria, both of which may be involved in thermogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01172869
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  • 10
    Electronic Resource
    Electronic Resource
    Sympathetic activation of brown-adipose-tissue thermogenesis in cachexia (1981)
    Springer
    Bioscience reports 1 (1981), S. 509-517 
    Details
    ISSN: 1573-4935
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Tumour-bearing mice spontaneously lose weight 8–9 weeks after implantation of a human hypernephroma, in spite of a normal food intake. Resting oxygen consumption was up to 40% higher in these animals than in sham-operated controls, but was significantly reduced by 8-adrenergic blockade with propranolol in the former group. The injection of noradrenaline caused a marked stimulation of the metabolic rate in all the animals, but the greatest response was seen in the cachectic mice. The brown-adipose-tissue mass was similar for both groups, but guanosine diphosphate binding to brownadipose-tissue mitochondria (an index of thermogenic capacity) was significantly increased in turnout-bearing mice, and the injection of noradrenaline 1 h prior to sacrifice caused the greatest stimulation of binding in the cachectic group. These data suggest that the rapid weight loss of tumour-bearing animals may be due to a high metabolic rate which results from sympathetic stimulation of brown-adipose-tissue metabolism. The relevance of these results to cancer-induced cachexia in man is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01121584
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