ALBERT

Description: 1. The relationship existing between the gross digestible energy content and the chemical composition of 24 hays has been studied with nine Cheviot wether lambs over a period of 8 months.2. Each hay was fed to a group of three lambs for a 10-day preliminary and 10-day collection period. Faeces and urine were collected in metabolism crates.

Description: 1. The relationship existing between the gross digestible energy content and the chemical composition of twenty-four silages has been studied with nine Cheviot wether lambs over a period of 8 months.2. Silages were made from grassland herbage and varied widely in the chemical composition of the ingoing material.3. Each silage was fed to a group of three lambs for a 10-day preliminary- and a 9-day collectionperiod. Faeces and urine were collected in metabolism crates.4. The silages were analysed for volatile acids and bases, for proximate nutrients and for some of the more precise chemical constituents as suggested in the scheme of analysis of Ferguson (1948).5. A close relationship existed between the gross digestible energy contents of the silages and the lignin (Ellis) content.6. The most accurate prediction of gross digestible energy was obtained by taking into account the content of lignin (Ellis), cellulose (Crampton & Maynard) and crude protein, in the silage.7. In the case of hays (Walker & Hepburn, 1955) no increase in the accuracy of predicting gross digestible energy was obtained by estimating the lignin and cellulose content, rather than crude fibre alone. However, with silages the results give support to the opinion of Mitchell (1942) and Schneider et al. (1951) that analysis for more precise chemical constituents will increase the accuracy of predicting digestibility.8. Crude fibre and cellulose were not related in any fixed way with the digestibility of energy. Crude fibre itself was digested to some 80% compared with 62% in hays.9. Metabolizable energy was closely related to digestible energy.10. The starch equivalents of the silages were calculated by conventional methods and also from the content of gross digestible energy.

Description: The physical properties of hardness and dry matter and the histological characteristics of dermal and epidermal tissues were investigated in lateral and medial claws obtained post mortem from mature crossbred female cattle. Claws containing pigmented (n=22) or non-pigmented (n=22) coronary wall horn were studied. Impression hardness, measured on the dorsal border of claws, proximo-distal at intervals of 5 mm from the lower perioplic line was shown to increase to the 20 mm lower site. Similarly, in comparison with values for pigmented horn, pooled measurements for non-pigmented horn showed greater hardness from the lower perioplic line to the 40 mm lower site but were not different for subsequent measurements towards the distal edge. Differences in values recorded for sole and heel and for coronary wall horn measured under the distal edge were not different for claws varying in coronary horn pigmentation. The absence of differences in dry matter at measurement sites suggested that differences in impression hardness values were not caused by variation in water content of horn. Histological examination of pigmented claw wall horn and underlying soft tissue showed pigment-containing cells in the coronary epidermis but not coronary dermis or in dermis or epidermis of laminae. Pigmentation appeared concentrated in melanosomes in cells along the basement membrane and was present uniformly throughout the epidermis and in cells lining horn tubules. There was no pigmentation detected in the soft tissue epidermis of claws selected visually for non-pigmented wall horn. The presence of melanosomes in epidermal cells from the pigmented coronary region was demonstrated by electron microscopy. The results suggest that pigmentation in the coronary region is associated with reduced initial development of hardness. The possible antagonism between antioxidant properties of melanins and requirement for an oxidative environment for disulphide bond formation in the cytoskeleton of horn cells is discussed.

Description: SUMMARYL-methionine is a sulphur-containing nutritionally essential amino acid. It has a number of important roles in epidermal and dermal tissues of the integument of animals. Failure of normal function of these tissues in the hoof (claw) is a cause of lameness in cattle. Little is known about quantitative relationships between post-absorptive concentrations of nutrients including sulphur-containing amino acids and uptake and utilization by epidermis and dermis of the bovine claw. These parameters were studied at the tissue level by use of an established in vitro claw explant system using tissue from cattle of beef or dairy origin and L-[35S]-labelled methionine as tracer. The results showed that uptake of L-methionine by freshly prepared solear explants in Dulbecco's Modified Eagle Medium/F-12 Nutrient Mix (DMEM/F12) (1:1) medium containing 1·0 mmol L-methionine/litre was concentrative after 5–8 min, essentially linear for up to 10 min and became curvilinear thereafter. Maximum uptake and steady state conditions were obtained at approximately 30 min. Further measurements were made following 21 h incubation in culture medium. Under conditions of varying concentrations of L-methionine and measurement of uptake after 30 min, the presence of a saturable curve, that obeyed Michaelis–Menten kinetics, was demonstrated. Values of 3·61 mmol/litre and 5·84 mmol/kg intracellular water/30 min were obtained for KM and Vmax, respectively. Uptake was not influenced by L-cysteine and L-cystine concentrations in the culture media.Similar culture and incubation conditions were used in subsequent studies of DNA and protein synthesis. These showed that rates of incorporation of L-methionine into protein fractions and stimulation of DNA synthesis measured by methyl-thymidine incorporation were dependent on L-methionine concentrations in the medium. Maximal rates occurred at approximately 50 μmol/litre, which is in the normal physiological range, and at 1% of maximum uptake capacity. Examination of histological sections by autoradiography showed localization of L-[35S]-labelled methionine in basal and suprabasal epidermal cells with limited retention in dermis. Measurement, by a range of histological, immunohistochemical, electrophoretic, western blotting and autoradiographic techniques, provided further evidence of L-methionine-dependent regulation of proliferation, differentiation and synthesis of proteins under physiological concentrations, by epidermal horn-forming cells.A key role for L-methionine is suggested in the production of horn in bovine claw. The extrapolation of these in vitro data provides guidance for strategies to optimize methionine supply to claw tissues in vivo. Such extrapolation suggests the appropriateness of delivery of systemic concentrations of 50 μmol L-methionine/litre to maximize proliferative and protein depositional activity in solear epidermis and dermis in vivo.