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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the World Aquaculture Society 24 (1993), S. 0 
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Frozen beef liver fed at dietary levels of 5, 10, 15, 20, and 25% (dry weight) to postlarval Mucrobruchium rosenbergii was evaluated as a supplement to Mna Trout Chow No. 2. Final mean wet weights and yields of prawns fed 15, 20, and 25% beef liver were significantly higher than those of prawns that received 5 and 10% levels. Survival rates and food conversion ratios of dry matter fed to wet biomass produced were not significantly different among treatments. The suitability of 47 fresh and frozen fruits and vegetables as dietary supplements was tested. Fourteen fruits and vegetables sank and were readily consumed by prawns, and seven (fresh orange and sweet potato, and frozen banana, carrot tops, butternut squash, yellow squash, and turnip greens) were tested as supplements (10% dry weight of the total ration) to trout chow and 15% beef liver. There were no significant differences (P 〈 0.05) in final mean weight, survival, yield, or food conversion ratio. Pigmentation of prams fed carrot tops or turnip greens was enhanced. No significant differences in final mean weight, yield, or survival were found when frozen banana was evaluated at levels of 0, 5,10,15, and 20% as a supplement to trout chow and 15% beef liver. Food conversion ratios for prawns fed 15 and 20% levels of banana were not different from those fed 10%, but were significantly higher than for prawns fed the 0 and 5% levels. Thus, a diet of Purina Trout Chow No. 2 plus 15% beef liver gives good results with postlarval M. rosenbergii in aquaria. Largescale testing and an economic analysis are needed to determine if this diet is practical for commercial prawn nurseries.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Differences in high-resolution two-dimensional gel electrophoresis patterns of micro-somal proteins from developing normal sunflower (Helianthus annuus L.) seeds before and after cold-induction, and also from normal and a high oleic sunflower mutant have been studied in order to detect the polypeptides associated with the microsomal Δl2-desaturase activity and its regulation by temperature. Proteins were obtained from developing seeds of two isogenic sunflower lines HA-89 (normal) and HA-OL9 (high oleic) which greatly differed in linoleic acid content and “in vitro” oleate desaturase activity. In the high oleic mutant, four polypeptides of about 32 kDa and two of 33 kDa were found to change in position, to the same extent, toward a lower isoelectric point in the high oleic mutant. Also, two polypeptides, of 32 and 49 kDa each, appeared in the mutant. Quantitative differences between cold-induced seeds (10°C, 24 h) and their non-induced controls were found. One polypeptide of 43 kDa decreased in the cold-treated seeds and two others, of 30 and 32 kDa each, increased markedly after cold induction. Some of these polypeptides could be related to oleate desatnrase activity or its regulation by temperature.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 45 (1998), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Degenerate primers for two regions of sequence homology in the myosin head domain were used in a polymerase chain reaction screen of Tetrahymena thermophila genomic DNA to amplify a 765 bp fragment that was cloned and sequenced. Based on the presence of conserved, myosin-specific sequences, the 765 bp PCR product was identified as a fragment of a myosin gene, the first to be discovered in ciliated protozoa and herein referred to as MYO1. An inverse polymerase chain reaction strategy was used to obtain additional sequence data that included the entire head domain of MYO1. Alignment of the predicted amino acid sequence of the MYO1 head domain with known myosin sequences identified the ATP-binding site, a phosphorylation site, and other myosin-specific consensus regions. In a northern blot analysis, a 765 bp MYO1-specific probe detected a 6.6 kb transcript under highly stringent hybridization conditions. Phylogenetic analysis revealed that the predicted protein encoded by MYO1 is not a member of any of the previously defined myosin classes and therefore represents a presumptive new myosin class.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 121 (2002), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: CAS-12 is a sunflower mutant with increased levels of palmitic (C16: 0 = 30%) and oleic (C18: 1 = 55%) acids in its seed oil, hence it has a reduced linoleic acid content (C18: 2 〈 5%). This study was conducted to determine the inheritance of high C16: 0 content and its relationship with high C18: 1 content in CAS-12. Reciprocal crosses involving CAS-12, CAS-5 (high C16: 0 content), HAOL-9 (high C18: 1 content) and HA-89 (standard fatty acid profile) were made. The F1, F2 and BC1F1 generations were obtained. The genetic control of the high C16: 0 trait in CAS-12 was partially recessive and gametophytic. In all cases, this character segregated in the ratio 19: 38: 7 (low: intermediate: high C16: 0 content) in the F2 generation. These results, together with the lack of segregation for C16: 0 content in crosses between CAS-12 and CAS-5, indicated that the genetic control of the high C16: 0 trait in CAS-12 was similar to that in CAS-5 in being controlled by partially recessive alleles (p1, p2, and p3) at three loci. Crosses between HA-89 and CAS-12, and HAOL-9 and CAS-5 (segregating for C16: 0 and C18: 1) demonstrated that the high C16: 0 and the high C18: 1 traits were independently inherited. However, C18: 1 segregation in these crosses exhibited reversal of dominance. Apparently, the low C18: 1 parental lines carried modifier genes causing the deviation.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 47 (2000), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Targeted gene disruption was used to investigate the function of MYO1, an unconventional myosin gene in Tetrahymena thermophila. Phenotypic analysis of a transformed strain that lacked a functional MYO1 gene was conducted at both 20°C and 35°C. At either temperature the ΔMYO1 strain had a smaller cytoplasm/nucleus ratio than wild type. At 20°C, ΔMYO1 populations had a longer doubling time than wild type, lower saturation density, and a reduced rate of food vacuole formation. However, at 35°C, these characteristics were comparable to wild type. Although micronuclear division and cytokinesis appeared normal in ΔMYO1 cells, failure of the macro-nucleus to elongate properly resulted in unequal segregation of macronuclear DNA in cells maintained at either 20°C or 35°C.
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  • 6
    Electronic Resource
    Electronic Resource
    Cambridge : Cambridge University Press
    Architectural research quarterly 4 (2000), S. 337-348 
    ISSN: 1359-1355
    Source: Cambridge Journals Digital Archives
    Topics: Architecture, Civil Engineering, Surveying
    Notes: Oficinas saliteras (nitrate works) were company towns developed to extract and process nitrates in the Antofagasta region of Chile's Atacama desert, one of the world's harshest environments. The two last of these oficinas were María Elena (founded in 1925) and Pedro de Valdivia (1931), whose development marked the introduction of the Guggenheim industrial system. Their urban design was closely related to the industrial cities of Europe and the United States, as well as the Spanish urban tradition. María Elena used an octagonal street plan, while Pedro de Valdivia is based on a crossing pattern.
    Type of Medium: Electronic Resource
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  • 7
    Publication Date: 2012-07-05
    Description: The karstic Lake Montcortès sedimentary sequence spanning the last 1548 yr constitutes the first continuous, high-resolution, multi-proxy varved record in northern Spain. Sediments consist of biogenic varves composed of calcite, organic matter and detrital laminae and turbidite layers. Calcite layer thickness and internal sub-layering indicate changes in water temperature and seasonality whereas the frequency of detrital layers reflects rainfall variability. Higher temperatures occurred in Lake Montcortès in AD 555–738, 825–875, 1010–1322 and 1874–present. Lower temperatures and prolonged winter conditions were recorded in AD 1446–1598, 1663–1711 and 1759–1819. Extreme and multiple precipitation events dominated in AD 571–593, 848–922, 987–1086, 1168–1196, 1217–1249, 1444–1457, 1728–1741 and 1840–1875, indicating complex hydrological variability in NE Spain since AD 463. The sedimentary record of Lake Montcortès reveals a short-term relation between rainfall variability and the detrital influx, pronounced during extended periods of reduced anthropogenic influences. In pre-industrial times, during warm climate episodes, population and land use increased in the area. After the onset of the industrialization, the relationship between climate and human activities decoupled and population dynamics and landscape modifications were therefore mostly determined by socio‐economic factors.
    Print ISSN: 0033-5894
    Electronic ISSN: 1096-0287
    Topics: Geography , Geosciences
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  • 8
    Publication Date: 2006-07-01
    Description: Palynological, sedimentological and stable isotopic analyses of carbonates and organic matter performed on the El Portalet sequence (1802 m a.s.l., 42°48′00ʺN, 0°23′52ʺW) reflect the paleoclimatic evolution and vegetation history in the central-western Spanish Pyrenees over the last 30,000 yr, and provide a high-resolution record for the late glacial period. Our results confirm previous observations that deglaciation occurred earlier in the Pyrenees than in northern European and Alpine sites and point to a glacial readvance from 22,500 to 18,000 cal yr BP, coinciding with the global last glacial maximum. The patterns shown by the new, high-resolution pollen data from this continental sequence, chronologically constrained by 13 AMS 14C dates, seem to correlate with the rapid climate changes recorded in Greenland ice cores during the last glacial–interglacial transition. Abrupt events observed in northern latitudes (Heinrich events 3 to 1, Oldest and Older Dryas stades, Intra-Allerød Cold Period, and 8200 cal yr BP event) were also identified for the first time in a lacustrine sequence from the central-western Pyrenees as cold and arid periods. The coherent response of the vegetation and the lake system to abrupt climate changes implies an efficient translation of climate variability from the North Atlantic to mid latitudes.
    Print ISSN: 0033-5894
    Electronic ISSN: 1096-0287
    Topics: Geography , Geosciences
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  • 9
  • 10
    Publication Date: 2002-09-01
    Description: Central Chile (32–35°S) lies at the northern border of strong Westerly influence and thus exhibits a steep precipitation gradient. Therefore, the palaeoclimatic archives in the region are suitable for detecting past moisture changes. The study of Laguna Aculeo (33°50'S, 70°54'W) presents a multiproxy Holocene lake record including sedimentology, geochemistry, mineralogy, pollen, diatoms, and radiocarbon dating (17 dates). Results indicate an arid early to mid-Holocene period (about 9500–5700 cal yr B.P.). After 5700 cal yr B.P. effective moisture increased progressively and around 3200 cal yr B.P., modern humid conditions were established. Numerous intercalated clastic layers reflect flood deposition during rainy winters. A fluvial unit was deposited shortly before 9000 cal yr B.P. Subsequently, flood events were absent until 5700 but have become frequent since 3200 cal yr B.P. The frequency of flood layers possibly points to weak or no El Niño activity during the early and mid-Holocene, with a subsequent increase during the late Holocene. During the early and mid-Holocene, the Westerlies were probably blocked and hence deflected southward by the subtropical high-pressure cell. Higher precipitation during the last 3200 yr seems strongly related to a weakened subtropical high-pressure cell with intensified Westerlies and possibly increased El Niño activity.
    Print ISSN: 0033-5894
    Electronic ISSN: 1096-0287
    Topics: Geography , Geosciences
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