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  • 1
    ISSN: 1365-2486
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Depletion of the stratospheric ozone layer has been of increased concern due to correlated increases in ambient ultraviolet radiation. Our research investigated the diel response of the motile behaviour of Nitzschia lineariz in both natural (freshwater stream) and experimental conditions. We classified the behaviour of individual diatom cells as gliding, immobile and oscillating. The experimental conditions were of simultaneous exposure either to net ambient solar radiation (control) or to ambient plus enhanced UV-B levels (treatment). UV-B for the control condition was filtered out using polyester filters; cells exposed to UV-B were irradiated through cellulose diacetate filters. UVB-313 levels were augmented to 33% (290 mW m–2) and 66% (365 mW m–2) above the local average ambient levels (220 mW m–2) for 10 h. The mobile behaviour of cells was examined and quantified every 2 consecutive hours from dawn to dusk in subsampled populations. The number of gliding cells in subsample populations declined on overcast days at both the 33 and 66% UV-B enhancement levels, whereas the number of immobile and oscillating cells increased with increased UV-B exposure. On sunny days, mobile behaviour was not affected at either enhancement level, which suggests that cellular repair mechanisms may be activated during sunny conditions.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2486
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Two experiments assessed the effect of current and elevated levels of ultravioletB (UV-B) radiation on forage crop production. The effect of current levels of UV-B radiation was assessed by comparing the growth of eight cultivars of four legume and four grass species for three growing seasons (1994–96) under simulated sward conditions in the field. An exclusion system using cellulose diacetate or polyester covers provided comparable growing conditions for the plants, except for the presence or absence of ambient UV-B radiation, respectively. The second experiment studied the effect of elevated levels of UV-B on eight cultivars of two legume and two grass species in the greenhouse under simulated sward conditions. Natural lighting with sufficient supplemental light was used to provide ideal growing conditions for a 16-h day length. Separate sets of UV lights were installed to provide UV-B levels at approximately the same intensity as would be found in mid summer, and 33% and 66% more than this value. A fourth treatment consisted of removing UV-B radiation by using a polyester filter. Plant production was measured in both experiments. In 1994, field herbage yields from all young grass and legume seedlings were not significantly affected by the exclusion of ambient UV-B radiation, with the exception of alfalfa. Intra-specific variations with alfalfa yields were found for reduced levels of UV-B radiation. In general, these trends persisted as stands matured during two post seeding years. In the second experiment, no significant differences were observed for all tested species with increasing levels of UV-B radiation, except with some alfalfa cultivars and one birdsfoot trefoil cultivar. Collectively, these results demonstrate that in the northern latitudes young and mature plants of the studied species are resistant to current and potentially higher levels of solar UV-B radiation, with the exception of some alfalfa cultivars. The yield of these cultivars increased under enhanced levels of UV-B radiation in the greenhouse and decreased when UV-B was excluded in the field.
    Type of Medium: Electronic Resource
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