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  • 1
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We previously identified HpuB, an 85 kDa Fe-repressible protein required for utilization of Fe from, and binding to, haemoglobin and the haemoglobin–haptoglobin complex. The gene for hpuB was cloned from Neisseria meningitidis strain DNM2 and the predicted amino acid sequence indicates that HpuB is an outer membrane receptor belonging to the TonB family of high-affinity transport proteins. A second open reading frame, predicted to encode a 34.8 kDa lipoprotein, was discovered 5′ to hpuB, and was designated hpuA. HpuA was identified in a total-membrane-protein preparation by construction of a mutant lacking HpuA. Acylation of HpuA was confirmed by [3H]-palmitic acid labelling of meningococci. Consensus promoter sequences were not apparent 5′ to hpuB. The hpuA insertion mutation exerted a polar effect, abolishing expression of hpuB, suggesting that hpuA and hpuB are co-transcribed. The 3.5 kb polycistronic hpuAB mRNA was identified and shown to be transcriptionally repressed by iron. The transcriptional start site was identified 33 nucleotides 5′ to the hpuA translational start site, appropriately positioned around consensus promoter and ferric uptake regulator (Fur)-box sequences. The structure of this operon suggests that HpuA–HpuB is a two-component receptor analogous to the bipartite transferrin receptor TbpB–TbpA.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2486
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Models of mass and energy exchanges between the biosphere and the atmosphere generally contain a nonlinear dependence between fluxes and model parameters, and thus estimation of these parameters from measurements in a heterogeneous landscape depends on the scale of the observations. The scale-dependence of a typical surface-exchange model (the CSIRO Biospheric Model, CBM) is examined using the diurnal variation of hourly fluxes of CO2, latent heat, sensible heat and soil heat. The fluxes were measured using micrometeorological techniques over six sites in a grazing/pasture system in SE Australia during a period of three weeks in 1995. Nonlinear parameter inversion was used to determine model parameters.Analysis of the covariance of the estimates of the parameters and the unexplained residuals of the model showed that a maximum of three or four parameters could be determined independently from the observations for all six sites. Estimates of a key model parameter, jmax, the mean of maximum potential electron transport rate of all leaves within the canopy, was best determined by the measurements of net CO2 flux at all sites examined. Measurements of ground heat flux provide little information about any of the model parameters in CBM.Because of nonlinearities in the surface exchange model, calculated fluxes will be in error if parameters for the component vegetation types are simply averaged in proportion to their areal fraction. The magnitude of these errors was examined for CBM using a hypothetical land surface consisting of two surface types, each with different parameter values. Predictions of net CO2, latent heat and ground heat fluxes using a linear combination of model parameters for the two surface types were quite similar with those found using optimal estimates of the parameters for the landscape, but were significantly poorer for sensible heat fluxes.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Rhizobium melioti DctD activates transcription from the dctA promoter by catalysing the isomerization of closed complexes between σ54-RNA polymerase holoenzyme and the promoter to open complexes. DctD must make productive contact with σ54-holoenzyme and hydrolyse ATP to catalyse this isomerization. To define further the activation process, we sought to isolate mutants of DctD that had reduced affinities for σ54-holoenzyme. Mutagenesis was confined to the well-conserved C3 region of the protein, which is required for coupling ATP hydrolysis to open complex formation in σ54-dependent activators. Mutant forms of DctD that failed to activate transcription and had substitutions in the C-terminal half of the C3 region were efficiently cross-linked to σ54 and the β-subunit of RNA polymerase, suggesting that they bound normally to σ54-holoenzyme. In contrast, some mutant forms of DctD with amino acid substitutions in the N-terminal half of the C3 region had reduced affinities for σ54 and the β-subunit in the cross-linking assay. These data suggest that the N-terminal half of the C3 region of DctD contains a site that may contact σ54-holoenzyme during open complex formation.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 2012-07-04
    Description: In order to investigate the magnetic force distribution, flow field distribution and temperature field distribution during continuous casting process of Al/Al bimetal round billet under multi-electromagnetic fields, a comprehensive mathematical model has been developed. The model is based on a combination of the commercial finite element package ANSYS and the commercial finite volume package FLUENT, with the former for calculation of the multi-electromagnetic fields and the latter for calculation of the magnetic driven fluid flow, heat transfer, temperature field and solidification. There was a good agreement between the calculate results and the experiment results. Comparison of the calculated results of the continuous casting process without electromagnetic with that of the continuous casting process under multi-electromagnetic fields indicated that velocity patterns, temperature profiles and the sump depth are modified remarkably by the application of multi-electromagnetic fi...
    Print ISSN: 1757-8981
    Electronic ISSN: 1757-899X
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 5
    Publication Date: 2018-12-20
    Description: In order to understand the difference of nutritional components between two species of Pyracantha Roem fruits in Sichuan Province, the contents of total sugar, Vc, protein, crude fibre, pectin, polyphenol and 12 kinds of main mineral elements in the fruits were determined by using the mature fruits of Pyracantha angustifolia and Pyracantha fortunaeana as material. The results showed that the basic nutrients of P. angustifolia and P. fortunaeana were complete. Except for crude fiber, other nutrients of P. angustifolia were higher than those of P. fortunaeana . The contents of 12 kinds of mineral elements in the two plants were abundant, and the contents of potassium (K), calcium (Ca), magnesium (Mg), zinc (Zn) and manganese (Mn) as beneficial mineral elements in P. angustifolia were higher than those of P. fortunaeana , while the contents of cadmium (Cd), chromium (Cr), nickel (Ni) and lead (Pb) as heavy metals in P. fo...
    Print ISSN: 1755-1307
    Electronic ISSN: 1755-1315
    Topics: Geography , Geosciences , Physics
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  • 6
    Publication Date: 2018-11-07
    Description: Inter-basin water diversion projects are a major way to redress demand-supply imbalance of water resources among regions and realize rational allocation and effective utilization of regional water resources. A scientific management system of inter-basin water diversion projects is the basis to ensure the project’s smooth progress and maximize its integrated efficiency. Basic principles for building of the management system for inter-basin water diversion projects are clearly defined herein, and an advisable plan for building of the management system for the project of diverting water from Datong River to Huangshui River (hereinafter referred to as Datong-Huangshui Water Diversion Project) is proposed to provide reference for the government in decision-making of the management system for this water diversion project.
    Print ISSN: 1755-1307
    Electronic ISSN: 1755-1315
    Topics: Geography , Geosciences , Physics
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  • 7
    Publication Date: 2018-11-07
    Description: The object of this work was to improve fireman’s fighting efficiency for winter rescue. Firstly, relative standard and background of fire protective clothing for rescue was reviewed. Secondly, different types of clothing were studied according to different structure. Thirdly, fire protective clothing with anti-icing performance and thermal retention performance for rescue was designed. Further study should focus on the new clothing’s actual application in northern east fire brigade.
    Print ISSN: 1755-1307
    Electronic ISSN: 1755-1315
    Topics: Geography , Geosciences , Physics
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  • 8
    Publication Date: 2017-05-20
    Description: At present, reliability assessment plays an important role in power systems. When the component failure probabilities are interval valued, common methods fail to achieve reasonable interval reliability assessment result of power systems. In this paper a novel approach based on the belief universal generating function (BUGF) is proposed to calculate the reliability indexes of power systems. Instead of giving a single-valued assessment result, a belief function and a plausibility function are exploited to calculate the lower and upper bounds of the loss of load probability (LOLP), the loss of load expectation (LOLE), the expected unsupplied load (EUL) and the expected unsupplied energy (EUE) in UGF, respectively. The proposed approach can track the correlation of the original data well and keep it to the end of the calculation. By using BUGF compared to other common methods to calculate the interval LOLP, LOLE, EUL, and EUE of IEEE-RTS 79, the results show the BUGF method can trac...
    Print ISSN: 1757-8981
    Electronic ISSN: 1757-899X
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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