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  • 1
    Electronic Resource
    Electronic Resource
    Antagonistic involvement of FIS and H-NS proteins in the transcriptional control of hns expression (1996)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 19 (1996), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Gel shift and DNase I footprinting experiments showed that Escherichia coli FIS (factor for inversion stimulation) protein binds to at least seven sites in the promoter region of hns. These sites extend from −282 to +25 with two sites, closely flanking the DNA bend located at −150 from the transcriptional startpoint, partly overlapping the H-NS binding sites involved in the transcriptional autorepression of hns. The interplay between FIS, H-NS and the hns promoter region were studied by examining the effects of FIS and H-NS on in vitro transcription of hns–cat fusions, as well as looking at the effect of FIS on preformed complexes containing H-NS and a DNA fragment derived from the hns promoter region. Taken together, our data suggest that in the cell, FIS and H-NS interact with the promoter region of hns and influence their respective interactions (possibly competing for the same binding site), eliciting antagonistic effects so that an interplay between these proteins might contribute to the transcriptional control of hns
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1996.436961.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Post-transcriptional regulation of CspA expression in Escherichia coli (1996)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 19 (1996), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The Escherichia coli cspA gene, encoding the major cold-shock protein CspA, was deprived of its natural promoter and placed in an expression vector under the control of the inducible λ PL promoter. After induction of transcription by thermal inactivation of the λ ts repressor, abundant expression of the product (CspA) was obtained if the cells were subsequently incubated at 10°C, but poor expression was obtained if the cells were incubated at 37°C or 30°C. The reason for this differential temperature-dependent expression was investigated and it was found that: (i) the CspA content of the cells decreased more rapidly at 37°C compared to 10°C, regardless of whether transcription was turned off by addition of rifampicin; (ii) both the chemical and functional half-lives of the cspA transcript were substantially longer at 10°C compared to 37°C; (iii) S30 extracts as well as 70S ribosomes prepared from cold-shocked cells translated CspA mRNA (but not phage MS2 RNA) more efficiently than equivalent extracts or ribosomes obtained from control cells grown at 37°C; and (iv) purified CspA stimulated CspA mRNA translation. Overall, these results indicate that a selective modification of the cold-shocked translational apparatus favouring translation of CspA mRNA, and an increased stability of this mRNA at low temperature, may play an important role in the induction of cspA expression during cold shock.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1996.362897.x
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  • 3
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    Glufosinate Rate and Timing for Control of Glyphosate-Resistant Rhizomatous Johnsongrass (Sorghum halepense) in Glufosinate-Resistant Soybean (2016)
    Hindawi
    Details
    Publication Date: 2016-02-29
    Description: Field studies were conducted over a three-year period (2011, 2012, and 2013) in Louisiana to evaluate the effect of glufosinate rate and timing on glyphosate-resistant (GR) rhizomatous johnsongrass control in glufosinate-resistant soybean. Treatments included glufosinate (0.5, 0.6, or 0.7 kg ai ha−1) applied alone POST1 (46 cm tall johnsongrass) and sequentially 3 (POST2) or 4 (POST3) wk after POST1 at 0.5 or 0.6 kg ha−1. Glufosinate (0.7 kg ha−1) applied POST1 controlled johnsongrass 77% at soybean harvest. Averaged across sequential application rate, delaying the sequential application from POST2 to POST3 increased control from 65 to 78% at harvest. Increasing sequential application rate from 0.5 to 0.6 kg ha−1 reduced johnsongrass heights 15% at harvest. Furthermore, delaying the sequential application from POST2 to POST3 reduced GR rhizomatous johnsongrass heights to 63% of the nontreated at harvest. Soybean yields were maximized following the POST1 application of glufosinate at 0.7 kg ha−1 (2670 kg ha−1) and by applying 0.6 kg ha−1 of glufosinate sequentially (2620 kg ha−1), regardless of sequential application timing. Maximum control and soybean yield were observed following glufosinate POST1 at 0.7 kg ha−1 followed by 0.6 kg ha−1 at POST3. This data indicates that glufosinate is an option for management of GR rhizomatous johnsongrass.
    Print ISSN: 1687-8159
    Electronic ISSN: 1687-8167
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Hindawi
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  • 4
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    Incompressible Turbulent Flow Simulation Using theκ-ɛModel and Upwind Schemes (2007)
    Hindawi
    Details
    Publication Date: 2007-01-01
    Description: In the computation of turbulent flows via turbulence modeling, the treatment of the convective terms is a key issue. In the present work, we present a numerical technique for simulating two-dimensional incompressible turbulent flows. In particular, the performance of the high Reynoldsκ-ɛmodel and a new high-order upwind scheme (adaptative QUICKEST by Kaibara et al. (2005)) is assessed for 2D confined and free-surface incompressible turbulent flows. The model equations are solved with the fractional-step projection method in primitive variables. Solutions are obtained by using an adaptation of the front tracking GENSMAC (Tomé and McKee (1994)) methodology for calculating fluid flows at high Reynolds numbers. The calculations are performed by using the 2D version of theFreeflowsimulation system (Castello et al. (2000)). A specific way of implementing wall functions is also tested and assessed. The numerical procedure is tested by solving three fluid flow problems, namely, turbulent flow over a backward-facing step, turbulent boundary layer over a flat plate under zero-pressure gradients, and a turbulent free jet impinging onto a flat surface. The numerical method is then applied to solve the flow of a horizontal jet penetrating a quiescent fluid from an entry port beneath the free surface.
    Print ISSN: 1024-123X
    Electronic ISSN: 1563-5147
    Topics: Mathematics , Technology
    Published by Hindawi
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  • 5
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    Stochastic Dynamic Programming Applied to Hydrothermal Power Systems Operation Planning Based on the Convex Hull Algorithm (2010)
    Hindawi
    Details
    Publication Date: 2010-01-01
    Description: This paper presents a new approach for the expected cost-to-go functions modeling used in the stochastic dynamic programming (SDP) algorithm. The SDP technique is applied to the long-term operation planning of electrical power systems. Using state space discretization, the Convex Hull algorithm is used for constructing a series of hyperplanes that composes a convex set. These planes represent a piecewise linear approximation for the expected cost-to-go functions. The mean operational costs for using the proposed methodology were compared with those from the deterministic dual dynamic problem in a case study, considering a single inflow scenario. This sensitivity analysis shows the convergence of both methods and is used to determine the minimum discretization level. Additionally, the applicability of the proposed methodology for two hydroplants in a cascade is demonstrated. With proper adaptations, this work can be extended to a complete hydrothermal system.
    Print ISSN: 1024-123X
    Electronic ISSN: 1563-5147
    Topics: Mathematics , Technology
    Published by Hindawi
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  • 6
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    Glufosinate Rate and Timing for Control of Glyphosate-Resistant Rhizomatous Johnsongrass (Sorghum halepense) in Glufosinate-Resistant Soybean (2016)
    Hindawi
    Details
    Publication Date: 2016-01-01
    Description: Field studies were conducted over a three-year period (2011, 2012, and 2013) in Louisiana to evaluate the effect of glufosinate rate and timing on glyphosate-resistant (GR) rhizomatous johnsongrass control in glufosinate-resistant soybean. Treatments included glufosinate (0.5, 0.6, or 0.7 kg ai ha−1) applied alone POST1 (46 cm tall johnsongrass) and sequentially 3 (POST2) or 4 (POST3) wk after POST1 at 0.5 or 0.6 kg ha−1. Glufosinate (0.7 kg ha−1) applied POST1 controlled johnsongrass 77% at soybean harvest. Averaged across sequential application rate, delaying the sequential application from POST2 to POST3 increased control from 65 to 78% at harvest. Increasing sequential application rate from 0.5 to 0.6 kg ha−1reduced johnsongrass heights 15% at harvest. Furthermore, delaying the sequential application from POST2 to POST3 reduced GR rhizomatous johnsongrass heights to 63% of the nontreated at harvest. Soybean yields were maximized following the POST1 application of glufosinate at 0.7 kg ha−1(2670 kg ha−1) and by applying 0.6 kg ha−1of glufosinate sequentially (2620 kg ha−1), regardless of sequential application timing. Maximum control and soybean yield were observed following glufosinate POST1 at 0.7 kg ha−1followed by 0.6 kg ha−1at POST3. This data indicates that glufosinate is an option for management of GR rhizomatous johnsongrass.
    Print ISSN: 1687-8159
    Electronic ISSN: 1687-8167
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Hindawi
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  • 7
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    Coding and Noncoding Gene Expression Biomarkers in Mood Disorders and Schizophrenia (2013)
    Hindawi
    Details
    Publication Date: 2013-01-01
    Description: Mood disorders and schizophrenia are common and complex disorders with consistent evidence of genetic and environmental influences on predisposition. It is generally believed that the consequences of disease, gene expression, and allelic heterogeneity may be partly the explanation for the variability observed in treatment response. Correspondingly, while effective treatments are available for some patients, approximately half of the patients fail to respond to current neuropsychiatric treatments. A number of peripheral gene expression studies have been conducted to understand these brain-based disorders and mechanisms of treatment response with the aim of identifying suitable biomarkers and perhaps subgroups of patients based upon molecular fingerprint. In this review, we summarize the results from blood-derived gene expression studies implemented with the aim of discovering biomarkers for treatment response and classification of disorders. We include data from a biomarker study conducted in first-episode subjects with schizophrenia, where the results provide insight into possible individual biological differences that predict antipsychotic response. It is concluded that, while peripheral studies of expression are generating valuable results in pathways involving immune regulation and response, larger studies are required which hopefully will lead to robust biomarkers for treatment response and perhaps underlying variations relevant to these complex disorders.
    Print ISSN: 0278-0240
    Electronic ISSN: 1875-8630
    Topics: Biology , Chemistry and Pharmacology
    Published by Hindawi
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