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  • Oxford University Press  (75)
  • American Chemical Society (ACS)  (35)
  • Blackwell Science Ltd
  • Nature Publishing Group (NPG)
  • 2010-2014  (103)
  • 2000-2004  (32)
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  • 1
    Electronic Resource
    Electronic Resource
    Direct and indirect transcriptional activation of virulence genes by an AraC-like protein, PerA from enteropathogenic Escherichia coli (2004)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 54 (2004), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The plasmid-encoded Per regulatory locus of enteropathogenic Escherichia coli (EPEC) is generally considered to consist of three genes, perA, perB and perC. PerA, a member of the AraC-like family of transcriptional regulators, is known to be an activator of its own promoter (autoactivation) as well as of the plasmid-located bfp operon encoding bundle-forming pili, but its role in activation of the chromosomal locus of enterocyte effacement (LEE) pathogenicity island, which confers the property of intimate adherence on EPEC, requires clarification. Here, we show that PerA is also required for activation of the master regulatory LEE operon, LEE1, but that this activation is indirect, being achieved via autoactivation of the per promoter which ensures sufficient production of the PerC protein to activate LEE1. In contrast, PerA-dependent activation of the per and bfp promoters is direct and does not require the other Per proteins, but is modulated by the nucleoid-associated protein H-NS. The closely related VirF regulator from Shigella flexneri cannot substitute for PerA to activate these promoters, despite being able to bind their upstream regions in vitro. PerA can bind the per and bfp promoter fragments to form multiple complexes, while VirF forms only a single complex. Site-directed mutagenesis of the PerA protein suggests that, like VirF, it may use both of its carboxy-terminal helix—turn–helix motifs for DNA interaction, and may also make direct contacts with RNA polymerase. In addition, we have isolated mutations in the poorly characterized amino-terminal domain of PerA which affect its ability to activate gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.2004.04333.x
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  • 2
    Electronic Resource
    Electronic Resource
    TlpC, a novel chemotaxis protein in Rhodobacter sphaeroides, localizes to a discrete region in the cytoplasm (2002)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 46 (2002), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: TlpC is encoded in the second chemotaxis operon of Rhodobacter sphaeroides. This protein shows some homology to membrane-spanning chemoreceptors of many bacterial species but, unlike these, is essential for R. sphaeroides chemotaxis to all compounds tested. Genomic replacement of tlpC with a C-terminal gfp fusion demonstrated that TlpC localized to a discrete cluster within the cytoplasm. Immunogold electron microscopy also showed that TlpC localized to a cytoplasmic electron-dense region. Correct TlpC–GFP localization depended on the downstream signalling proteins, CheW3, CheW4 and CheA2, and was tightly linked to cell division. Newly divided cells contained a single cluster but, as the cell cycle progressed, a second cluster appeared close to the initial cluster. As elongation continued, these clusters moved apart so that, on septation, each daughter cell contained a single TlpC cluster. The data presented suggest that TlpC is either a cytoplasmic chemoreceptor responding to or integrating global signals of metabolic state or a novel and essential component of the chemotaxis signalling pathway. These data also suggest that clustering is essential for signalling and that a mechanism may exist for targeting and localizing proteins within the bacterial cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2002.03252.x
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  • 3
    Electronic Resource
    Electronic Resource
    The third chemotaxis locus of Rhodobacter sphaeroides is essential for chemotaxis (2002)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 46 (2002), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The purple photosynthetic bacterium Rhodobacter sphaeroides has three loci encoding multiple homologues of the bacterial chemosensory proteins: 13 putative chemoreceptors, four CheW, four CheA, six CheY, two CheB and three CheR. Previously, studies have shown that, although deletion of cheOp1 led to only minor changes in behaviour, deletion of cheOp2 led to a loss of taxis. The third locus encodes two CheA, one CheR, one CheB, one CheW, one CheY, a putative cytoplasmic chemoreceptor (TlpT) and a protein showing homology to the chromosomal partitioning factor Soj (designated Slp). Here, we show that every protein encoded by this locus is essential for normal chemotaxis. Phototaxis is also dependent upon all the components of this locus, except CheB2 and Slp. The two putative CheA proteins encoded in this locus are unusual. CheA3 has only the P1 domain and the P5 regulatory domain linked by a large internal domain, whereas CheA4 lacks the P1 and P2 domains required for phosphorylation and response regulator binding. These data indicate that the minimal set of proteins required for normal chemotaxis in R. sphaeroides is all the proteins encoded by cheOp2 and the third chemotaxis locus, and that the multiple chemosensory protein homologues found in R. sphaeroides are not redundant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2002.03218.x
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  • 4
    Electronic Resource
    Electronic Resource
    Ozone effects on wheat in relation to CO2: modelling short-term and long-term responses of leaf photosynthesis and leaf duration (2000)
    Oxford, UK : Blackwell Science Ltd
    Global change biology 6 (2000), S. 0 
    Details
    ISSN: 1365-2486
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: A combined stomatal–photosynthesis model was extended to simulate the effects of ozone exposure on leaf photosynthesis and leaf duration in relation to CO2. We assume that ozone has a short-term and a long-term effect on the Rubisco-limited rate of photosynthesis, Ac. Elevated CO2 counteracts ozone damage via stomatal closure. Ozone is detoxified at uptake rates below a threshold value above which Ac decreases linearly with the rate of ozone uptake. Reduction in Ac is transient and depends on leaf age. Leaf duration decreases depending on accumulated ozone uptake. This approach is introduced into the mechanistic crop simulation model AFRCWHEAT2. The derived model, AFRCWHEAT2-O3, is used to test the capability of these assumptions to explain responses at the plant and crop level.Simulations of short-term and long-term responses of leaf photosynthesis, leaf duration and plant and crop growth to ozone exposure in response to CO2 are analysed and compared with experimental data derived from the literature. The model successfully reproduced published responses of leaf photosynthesis, leaf duration, radiation use efficiency and final biomass of wheat to elevated ozone and CO2. However, simulations were unsatisfactory for cumulative radiation interception which had some impact on the accuracy of predictions of final biomass. There were responses of leaf-area index to CO2 and ozone as a result of effects on tillering which were not accounted for in the present model. We suggest that some model assumptions need to be tested, or analysed further to improve the mechanistic understanding of the combined effects of changes in ozone and CO2 concentrations on leaf photosynthesis and senescence. We conclude that research is particularly needed to improve the understanding of leaf-area dynamics in response to ozone exposure and elevated CO2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2486.2000.00351.x
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  • 5
    Electronic Resource
    Electronic Resource
    Distribution of benthic algae in the upper Illinois River basin in relation to geology and land use (2000)
    Oxford, UK : Blackwell Science Ltd
    Freshwater biology 44 (2000), S. 0 
    Details
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 〈list style="custom"〉1Benthic-algal distributions in the upper Illinois River basin, IL, U.S.A., were examined in relation to geology, land use, water chemistry and stream habitat using (detrended) (canonical) correspondence analysis, autecological metrics and indicator-species analysis in order to identify the major environmental gradients influencing community variation.2Ionic composition and major nutrient [i.e. nitrogen (N) and phosphorus (P)] concentration of surface waters, salinity (Na-Cl type), substratum type and physiognomic form of dominant species were primary factors contributing to variation in benthic-algal assemblages of the basin. Basin geology was a significant contributing factor, but the explained variance associated with this factor was less than that related to land use.3Proportions of algal biomass consisting of cyanophytes, filamentous chlorophytes, halophilic diatoms and diatoms which utilize nitrogen heterotrophically were greater in eutrophic river segments than in less nutrient-enriched segments. Composition of the benthic flora indicated meso-eutrophic or eutrophic conditions throughout the basin; there were few diatoms indicative of hypertrophic waters. Shifts in diatom-assemblage structure in response to nutrient loading provided an incomplete representation of the community-response curve.4A weighted-averages regression model based on total P and benthic-algal abundances (all divisions included) yielded a highly significant correlation (r2 = 0.83) between species-inferred [WA(tol)] and observed total P, with systematic bias (increased deviation of residuals) occurring only at concentrations greater than ∼ 1.0 mg L−1 total P. This result indicates that total P regression and calibration models can be predictable for a river basin receiving excessive loadings of phosphorus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2427.2000.00536.x
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  • 6
    Electronic Resource
    Electronic Resource
    The effects of high-concentrate diets and pasture on the concentration of conjugated linoleic acid in beef muscle and subcutaneous fat (2003)
    Oxford, UK : Blackwell Science Ltd
    Grass and forage science 58 (2003), S. 0 
    Details
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In view of the evidence indicating several potential benefits of high intakes of conjugated linoleic acid (CLA) on human health, an experiment was conducted to examine the effects of the diet of beef cattle on the total CLA concentration in muscle and subcutaneous fat. High-concentrate [steers consumed 515 g concentrates and 485 g grass silage kg−1 dry matter (DM) for 8 weeks followed by 887 g concentrates and 113 g grass silage kg−1 DM for 14 weeks until slaughter] and high-forage (steers consumed 803 g grass silage and 197 g concentrates kg−1 for 8 weeks followed by grass silage only for 10 weeks and then grazed perennial ryegrass-based pasture for 23 weeks until slaughter) treatments were imposed on 48 steers, which were crosses of continental beef breeds (initially 414 kg live weight) The concentrates were based on barley, extracted soyabean meal, molassed sugarbeet pulp and maize meal. The silages were of medium to low digestibility and contained 117–137 g crude protein kg−1 DM and 83–158 g ammonia-N kg−1 total N. The pasture was of high quality and contained 168 g crude protein, 234 g acid-detergent fibre and 222 g water-soluble carbohydrate kg−1 DM. Samples of muscle were taken post slaughter from the m. semimembranosus, m. gluteobiceps, m. longissimus and m. deltoideous muscles, and subcutaneous fat was taken from over the m. longissimus. Concentrations of total CLA in the tissues were for the high-concentrate and high-forage treatments, respectively (mg 100 g−1 fresh tissue), m. gluteobiceps 18 and 47 (s.e. 3·5); m. semimembranosus 9 and 20 (s.e. 1·6); m. longissimus 15 and 35 (s.e. 3·2), m. deltoideous 20 and 59 (s.e. 4·3); subcutaneous fat 584 and 1975 (s.e. 138·7). It is concluded that muscle and subcutaneous fat tissue from grass-fed cattle contained three times as much CLA as those from concentrate-fed cattle, and that the consumption of beef from grass-fed cattle should be effective in increasing the intake of CLA by humans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2494.2003.00351.x
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  • 7
    Electronic Resource
    Electronic Resource
    Application of cellular and immunological biosensor techniques to assess herbicide toxicity in soils (2002)
    Oxford, UK : Blackwell Science Ltd
    European journal of soil science 53 (2002), S. 0 
    Details
    ISSN: 1365-2389
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: There is growing concern about the fate and toxicity of herbicides to non-target receptors and an increasing need to measure these analytes sensitively. The responses of cellular and immunological biosensors to four commonly used herbicides (atrazine, diuron, mecoprop and paraquat) were investigated. In combination, these sensors assess toxicity and quantify concentrations of herbicides present in extracts from soil. The bioluminescence response of the lux-marked bacterial biosensor Escherichia coli HB101 was determined in aqueous extracts from soil to indicate toxicity. Smaller concentrations caused a toxic response for all four herbicides recovered from the Insch series than for those recovered from spiked water samples, but this was not a result of biodegradation of herbicides in the soil. This suggests that intrinsic soil factors may be altering the bioavailable fraction of herbicides, making them more toxic than equivalent concentrations in water.Herbicide concentrations were determined using immunological biosensors consisting of stabilized recombinant single chain antibodies (stAbs) specific for the four different groups of herbicides. These stAb fragments retain functionality in organic solvents such as methanol commonly used in soil extraction. Anti-atrazine, mecoprop, diuron and paraquat stAbs were successfully used to identify and quantify herbicides present in aqueous and methanol extracts from soil. The amounts recovered from immunoassay analysis were compared with chemical analysis using high performance liquid chromatography, and the two methods correlated. These stAb fragments might provide a more rapid and sensitive means of quantifying trace amounts of herbicides and their metabolites in aqueous and methanol extracts from soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2389.2002.00421.x
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  • 8
    Electronic Resource
    Electronic Resource
    Effects of the proportions of high or medium digestibility grass silage and concentrates in the diet of beef cattle on liveweight gain, carcass composition and fatty acid composition of muscle (2002)
    Oxford, UK : Blackwell Science Ltd
    Grass and forage science 57 (2002), S. 0 
    Details
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An experiment was carried out over 2 years to evaluate the effects of increasing the proportion of cereal-based concentrates in diets containing high-digestibility and conventional medium-digestibility grass silages on the dry-matter (DM) intake, liveweight gain and carcass composition of beef cattle, and to examine the effects of grazed grass and the ratio of grass silage:concentrates in the diet on the fatty acid composition of selected muscle tissues. Late-maturing steers (n = 231) were offered diets based on high-digestibility (HD) (0·743 digestible organic matter (DOM) in DM) or medium-digestibility (MD) (0·643 DOM in DM) grass silages supplemented with barley/soyabean meal-based concentrates. The concentrates constituted 0·20, 0·40, 0·60 and 0·80 of total DM of the diets, which were offered ad libitum (AL). The two diets, which contained 0·80 concentrates, were also offered at 0·80 of AL intake. A further group of fourteen animals were given the medium-digestibility silage only for 5 months and then grazed perennial ryegrass pastures for a further 5 months (silage/pasture treatment). For the diets containing HD silage and 0·20, 0·40, 0·60 and 0·80 concentrate, and 0·80 concentrate at 0·8 of AL intake, the DM intakes were 9·4, 10·2, 10·4, 10·2 and 8·1 (s.e. 0·16) kg d−1, respectively, and daily carcass gains were 0·67, 0·78, 0·77, 0·79 and 0·62 (s.e. 0·029) kg d−1, respectively; for those containing MD silage and 0·20, 0·40, 0·60 and 0·80 concentrate, and 0·80 concentrate at 0·8 of AL, the DM intakes were 8·2, 9·3, 10·1, 10·1 and 8·0 (s.e. 0·16) kg d−1, respectively, and daily carcass gains were 0·38, 0·48, 0·64, 0·77 and 0·56 (s.e. 0·029) kg d−1 respectively. Increasing the proportion of concentrates in silage-based diets decreased the concentration of omega-3 (ω-3) polyunsaturated fatty acids (PUFA) (P 〈 0·001) and increased the concentration of ω-6 PUFA (P 〈 0·001) in muscle. Cattle on the silage/pasture treatment had the highest concentration of ω-3 PUFA in muscle (51 g kg−1 lipid), this value being over three times that for animals given diets containing MD silage and 0·80 concentrate in the diet. These results demonstrate the potential of HD silage made from perennial ryegrass relative to high concentrate diets. The consumption of pasture-finished beef could make a significant contribution towards increasing the intake of ω-3 PUFA in the human diet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2494.2002.00326.x
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  • 9
    Electronic Resource
    Electronic Resource
    The role of air in fatigue load interaction (2003)
    Oxford, UK : Blackwell Science Ltd
    Fatigue & fracture of engineering materials & structures 26 (2003), S. 0 
    Details
    ISSN: 1460-2695
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Natural fatigue crack formation and growth were studied in notched Al–Cu alloy coupons through high-resolution SEM fractography. The experiments were conducted under programmed loading conditions designed to induce microscopic marking of the crack formation and growth process under varying stress ratio and closure-free crack tip conditions. Control experiments were performed by switching between an air and vacuum environment. In air, varying the stress ratio from 0.74 down to 0.64 retards crack growth by up to a factor of five. This ‘closure-free’ stress ratio history effect totally disappears in vacuum, suggesting a significant environmental influence on stress ratio and its history. Crack-tip stress state appears to moderate environmental action, revealing a potential mechanism sensitive to residual stress. Consequently, crack closure, residual stress and crack front and plane orientation are identified as major load interaction mechanisms whose synergistic action controls fatigue under variable amplitude loading. The study also appears to suggest that as a consequence of the crack seeking the path of least resistance, load-sequence sensitive crack plane and front orientation may only induce retardation effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1460-2695.2003.00582.x
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  • 10
    Electronic Resource
    Electronic Resource
    Fatigue voids and their significance (2002)
    Oxford, UK : Blackwell Science Ltd
    Fatigue & fracture of engineering materials & structures 25 (2002), S. 0 
    Details
    ISSN: 1460-2695
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Fractures from tests on 2014-T6511 and 2024-T3 test coupons under specially designed programmed loading reveal voids with distinct fatigue markings. These ‘fatigue voids’ appear to form as a consequence of the separation of noncoherent secondary particulates from the matrix in early fatigue. The process of their formation is through the initiation, growth and coalescence of multiple interfacial cracks around the particulate. Such voids become visible on the fatigue fracture surface if and when the crack front advances through them. In vacuum, each fatigue void is the potential initiator of an embedded penny-shaped crack. The one closest to the specimen surface is likely to become the dominant crack, indicating that fatigue voids appear to be the likely origins of the dominant crack in vacuum. In air, the dominant crack forms at the notch surface and grows much faster, giving less opportunity for multiple internal cracks to spawn off from the innumerable internal fatigue-voids. Thus in air, fatigue voids do not appear to affect the fatigue process at low and intermediate growth rates. At high crack growth rates involving considerable crack tip shear, slip planes with particulate concentration offer the path of least resistance. This explains the increasing density of fatigue voids with growth rate. Very high growth rates signal the onset of a quasi-static crack growth component that manifests itself through growing clusters of microvoid coalescence associated with static fracture. Fatigue voids are likely to form in other Al-alloys with secondary noncoherent particulates. They have nothing in common with microvoids associated with ductile fracture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1460-2695.2002.00565.x
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