ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
: Lysozyme from egg white was purified using the anionic surfactant, di-(2-ethylhexyl) sodium sulfosuccinate (Aerosol-OT or AOT), as a precipitating ligand. Upon addition, AOT formed an insoluble lysozyme-surfactant complex with lysozyme and precipitated from the egg white solution. The lysozyme was then recovered as a solid from the insoluble complex using acetone, while AOT remained in the acetone phase. The precipitation efficiency was highest when the pH of the egg white solution was between 6 and 9. A molar ratio of 15 between surfactant and lysozyme provided 80% precipitation efficiency. The recovered lysozyme product was free of surfactant, and retained its original enzymatic activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.2003.tb05716.x
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