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  • 1
    Electronic Resource
    Electronic Resource
    Preliminary evidence that different domains are involved in cytolytic activity and receptor (cholesterol) binding in listeriolysin O, the Listeria monocytogenes thiol-activated toxin (1989)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 65 (1989), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The inactive truncated 52 Kilodaltons (kDa) Listeriolysin O (LLO) produced by a transposon Tn 1545-induced Listeria monocytogenes non-hemolytic/avirulent mutant previously described (Gaillard et al. (1986) Infect. Immun. 52, 55-55), that lacks a 48 aminoacid fragment at the C-terminal end including the single cysteine residue essential for activity (Mengaud et al. (1988) Infect. Immun. 56, 766–772), bound to the SH-cytolysin membrane receptor cholesterol, as did the active 60 kDa toxin. These results indicate that the missing fragment is a functionally important region needed in the 60 kDa LLo to cause membrane-disruption but not to bind to cholesterol, which strongly suggests that in LLO (and presumably in the other SH-cytolysins, in accordance with their structural and functional homologies) different domains are involved in cytolytic activity and cholesterol binding. The cysteine residue contained in the missing fragment, therefore, would not be essential for cholesterol binding, as in currently thought, rather it seems to be essential specifically for cell lysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1989.tb03603.x
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  • 2
    Electronic Resource
    Electronic Resource
    Tectonic deformation of the Andes and the configuration of the subducted slab in central Peru; results from a microseismic experiment (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Geophysical journal international 103 (1990), S. 0 
    Details
    ISSN: 1365-246X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: A microearthquake survey was conducted in the central Andes of Peru, east of the city of Lima, to study the seismicity and style of tectonic deformation of the Peruvian Andes. Although most of the stations forming the temporary seismographic network were located on the high Andes, the vast majority of the microearthquakes recorded occurred to the east of the mountain belt: on the Huaytapallana fault in the Eastern Cordillera and beneath the western margin of the sub-Andes. Thus the sub-Andes appear to be the physiographic province subject to the most intense seismic deformation. Focal depths of the crustal events in this region range generally from 15 to 35 km and some events beneath the sub-Andes appear to be as deep as 40-50 km. The fault-plane solutions of events in the sub-Andean margin show thrust faulting on steep planes oriented roughly north-south, similar to that observed in teleseismic earthquakes studied using body wave modelling. The Huaytapallana fault in the Cordillera Oriental also shows relatively high seismicity along a NE-SW trend that agrees with the fault scarp and the east-dipping nodal plane of two large earthquakes that occurred on this fault on 1969 July 24 and October 1. Microearthquakes of intermediate depth recorded during the experiment show a flat seismic zone about 25 km thick at a depth of about 100 km. This agrees with recent observations showing that beneath Peru the slab first dips at an angle of about 30° to a depth of 100 km and then flattens following a quasi-horizontal trajectory. Fault-plane solutions of intermediate-depth microearthquakes have horizontal T axes oriented east-west suggesting slab pull is the dominant force in the downgoing slab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-246X.1990.tb01747.x
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  • 3
    Electronic Resource
    Electronic Resource
    Revision of the antigenic structure of genus Listeria (1990)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 67 (1990), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: O-antigenic structure of genus Listeria was studied, using antisera (obtained from rabbits) against different O-antigens of reference strains of each serovar. The titres of sera were determined by agglutination using antigens of the same reference strains as well. Some differences from the actual scheme were found: serum antifactor-IX gave a lower titre than expected against antigens 4ab and 6b, while the titre observed against antigen 4b was higher than the expected in this case. Serum antifactor-VIII presented a higher titre than could be expected against antigen 6b. The strains of serovars 4d and 4e used in this experience were impossible to distinguish, and could have been classified in the same serovar. We could not obtain serum antifactor-XI factor from serovar 6b after several trials. From these differences we propose some modifications of the current antigenic scheme of genus Listeria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1990.tb13846.x
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