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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Texas and Argentine bluegrass are both dioecious Poa species with distinct morphological characteristics. Argentine bluegrass has a bunch-type growth habit, fine upright leaves and does not produce rhizomes. Texas bluegrass spreads by rhizomes and has wider, longer leaves. In an effort to produce novel germplasm with forage or turf potential, experiments were conducted to determine whether hybrid seed could be produced. Crosses were made using pollen from an Argentine male to fertilize two Texas female plants. Parents were induced to flower in the greenhouse by extending the photoperiod to 18 h with 400 W high-pressure sodium lamps from late December to April. The two crosses produced 〉200 seeds each. Hybrid seedlings segregated for traits from both parents. A sorghum-derived SSR marker confirmed the hybrid nature of a group of seedlings. Seeds harvested from F1 plants using Texas or Texas × Argentine pollen lost the undesirable cottony characteristic of Texas bluegrass. Evaluations for forage and turf potential of hybrid and later generation seed are planned.
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  • 2
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The Phanerochaete chryososporium trpC gene has been isolated by complementation of an Escherichia coli trpC mutant. The full extent of the fungal gene, determined by sequence analysis, was found to be 2414bp. This includes a single intron of 50bp, the presence of which was confirmed by RNA-primed polymerase chain reaction analysis. This feature makes the P. chrysosporium gene unique when compared to equivalent genes from other filamentous fungi. The P. chrysosporium trpC gene encodes a single protein containing three enzyme activities involved in tryptophan biosynthesis arranged in the order: NH2–GAT–IGPS–PRAI–COOH. This order is conserved in all filamentous fungi so far examined and, indeed, is the gene order within the E. coli trp operon.
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  • 3
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: As a step towards indentifying exploitable differences between host and parasite at the molecular level, we have isolated and sequenced genomic clones encompassing an entire α-tubulin gene (designated α-tubulin I) from the human malaria parasite, Plasmodium falciparum. The gene, which contains two introns, encodes a product with a predicted length of 453 amino acid residues (50.3 kD). The protein sequence shows a high degree of homology to other α-tubulins, particularly that of the coccidian parasite, Toxoplasma gondii (94%), whose gene carries introns in identical positions. Only one copy of the α-tubulin I gene itself was found, although a second gene designated α-II was also identified which is closely related but which differs at both the nucleotide and amino acid sequence levels. The α-I and β-tubulin genes were found to reside on different chromosomes.
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