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  • 1
    Electronic Resource
    Electronic Resource
    Properties of Trypsin from the Pyloric Ceca of Atlantic Cod (Gadus morhua) (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 55 (1990), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Trypsin (EC 3.4.21.4) was isolated from the pyloric ceca of Atlantic cod and purified to homogeneity by affinity chromatography. The enzyme catalyzed the hydrolysis of benzoyl arginine p-nitroanilide (BAPA, pH 8.2 and 25°C) such that Vmax was 250 BAPA units per micromole trypsin and Km was 1.48 mM. For the hydrolysis of tosyl arginine methyl ester (TAME, pH 8.1 and 25°C), Vmax was 18.2 × 103 TAME units/micromole trypsin, and Km 0.22 mM. The pH and temperature optima with BAPA substrate were 7.5 and 40°C, respectively. Atlantic cod trypsin was most active and stable at alkaline pH. The enzyme was heat labile, losing more than 50% of its activity after incubation at 50°C for 30 min. Amino acid analysis of Atlantic cod trypsin revealed that the enzyme was rich in residues such as serine, glycine, glutamate and aspartate, but poor in basic amino acid residues compared to trypsins from warm blooded animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1990.tb01574.x
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  • 2
    Electronic Resource
    Electronic Resource
    Pulse-Labeling of Kinetoplast DNA: Localization of 2 Sites of Synthesis Within the Networks and Kinetics of Labeling of Closed Minicircles (1976)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 23 (1976), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Short pulse-labeling of log phase Crithidia fasciculata cells with [3H]thymidine allowed the autoradiographic visualization of 2 sites of replication of kinetoplast DNA situated at the periphery of the networks and separated by 180°. Longer pulse-labeling led to the previously reported total peripheral labeling pattern. Pulse-labeled networks possess an intermediate density in ethidium bromide-CsCl equilibrium gradients between the densities characteristic of closed networks and open or linear DNA. Removal of ethidium bromide by several methods and treatment of intermediate band networks with RNase and pronase had no effect on the equilibrium rebanding pattern. Closed minicircles of Leishmania tarentolae are not labeled by a short pulse of intact cells with [3H]thymidine. A chase of ∼ 3–4 hr is required for the appearance of radioactivity in closed minicircles, a time delay which implies the existence of intermediate events between replication and eventual covalent closure of the minicircles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1976.tb03846.x
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  • 3
    Electronic Resource
    Electronic Resource
    Labeling of Crithidia fasciculata DNA with [3H]Thymidine (1974)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 21 (1974), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Attempts at continuous labeling of Crithidia fasciculata DNA with [3H]thymidine led to a pulse-chase situation due to a cell-mediated conversion of thymidine to thymine in the medium. The uptake of thymine was slow compared to that of thymidine. Neither the addition of deoxyadenosine nor the sequential addition of several aliquots of [3H]thymidine had an effect on the pattern of labeling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1974.tb03674.x
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  • 4
    Electronic Resource
    Electronic Resource
    Trypanosoma brucei: Differentiation of in Vitro-Grown Bloodstream Trypomastigotes into Procyclic Forms (1985)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 32 (1985), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Trypanosoma brucei strain 366D trypomastigotes grown at 37°C in the presence of a human fibroblast cell line formed foci underneath the feeder cells whereas trypanosomes grown in the presence of a human epithelial cell line grew only in the culture supernatant. A culture system was developed to study the differentiation of bloodstream trypomastigotes grown in the epithelial cell system into procyclic trypomastigotes at 27°C. The morphological differentiation into the procyclic form was complete by 48 h. Cell division did not occur until 30–40 h after transfer to 27°C. Various characteristics of this system were examined, including the effect of the feeder layer, the type of medium, the presence of the metabolites cis-aconitate and citrate, the preadaptation period, and the trypanosome cell concentration. The respiration of the recently differentiated procyclic cells was less sensitive to inhibition by CN-than that of established procyclic forms, implying a delayed appearance of complete mitochondrial oxidative pathways. This trypanosome differentiation system has the advantage that the animal host is not needed and the entire process is carried out in in vitro culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1985.tb03100.x
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and Characterization of Kinetoplast DNA Networks and Minicircles from Crithidia fasciculata (1974)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 21 (1974), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Covalently closed kinetoplast DNA networks have been isolated from stationary phase Crithidia fasciculata cells by a technic involving selective pelleting of the networks at a low centrifugal field. Approximately 62% of the kinetoplast DNA of the cell was recovered free of nuclear DNA by simple differential centrifugation.Purified kinetoplast DNA networks were visualized both in the electron microscope and in the light microscope. Closed networks sedimented as a homogeneous band both in neutral and alkaline sucrose, with an s20w in neutral sucrose of approximately 5 × 103.Closed monomeric minicircles were isolated from purified networks by mild sonication and band sedimentation in alkaline sucrose. Several physical properties of closed monomeric minicircles were measured. These included molecular weight, buoyant density in CsCl, superhelix density and sedimentation coefficient.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1974.tb03751.x
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  • 6
    Electronic Resource
    Electronic Resource
    The Appropriate-Technology Question in a Regional Context (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Growth and change 14 (1983), S. 0 
    Details
    ISSN: 1468-2257
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geography , Economics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1468-2257.1983.tb00421.x
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  • 7
    Electronic Resource
    Electronic Resource
    The development of skilled work in the British coal mining industry, 1870–1985 (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Industrial relations journal 17 (1986), S. 0 
    Details
    ISSN: 1468-2338
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Economics
    Notes: Here the trajectory of manual work in the British coal mining industry since the late nineteenth century is examined in relation to five sociological models of development of modern skilled work. Each of the models is examined in the four main phases of the labour process in coal mining.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1468-2338.1986.tb00549.x
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  • 8
    Electronic Resource
    Electronic Resource
    Nucleotide sequence and analysis of the gene in Borrelia burgdorferi encoding the immunogenic P39 antigen (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 119 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The P39 antigen is a specific, highly conserved, and immunogenic protein of Lyne disease spirochetes, Borrelia burgdorferi sensu lato. The nucleotide sequence of the gene encoding this protein was determined and found to be the first of two tandemly arranged open reading frames located on the spirochete's chromosome. These two open reading frames were designated bmpA for the gene encoding P39 and bmpB for the gene encoding the putative protein ORF2 encoded by the second open reading frame. The nucleic acid sequence identity for the two open reading frames was 62% while their deduced amino acid sequences were 52% identical. Comparison to sequence data bases demonstrated that the deduced amino acid sequences of both P39 and ORF2 were homologous to TmpC, a putative outer or cytoplasmic membrane lipoprotein of the syphilis spirochete, Treponema pallidum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb06917.x
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  • 9
    Electronic Resource
    Electronic Resource
    Effect of growth temperature on the composition of the photosynthetic apparatus in a thermophilic cyanobacterium (Synechococcus sp.) (1989)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 57 (1989), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A thermophilic strain of Synechococcus sp. was grown under light-limited conditions at its optimum temperature of 58°C and at 38°C in order to investigate the effect of growth temperature on the composition of the photosynthetic apparatus. Cells grown at 38°C had a ratio of Photosystem I to Photosystem II of 2.2, close to that known to occur in unstressed mesophilic strains of Synechococcus. Growth at the higher temperature led to a doubling of the number of Photosystem I reaction centres per cell whilst Photosystem II remained essentially unchanged, causing the Photosystem I/Photosystem II ratio to rise to 4.1. These changes were correlated with an increase in the number of concentric layers of thylakoid membranes from four to nine. It is suggested that these changes result from a higher relative demand for energy (ATP) during growth at elevated temperatures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1989.tb03217.x
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  • 10
    Electronic Resource
    Electronic Resource
    Experimental Vibrio cholerae wound infections (1987)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 40 (1987), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The invasive potential of environmental and clinical strains of O1 and non-O1 Vibrio cholerae was examined. Mice injected subcutaneously (s.c.) with strains of these organisms were monitored for the development of lesions or mortality. Of 12 strains of non-O1 V. cholerae tested, 7 gave a high mortality rate. All other strains produced swelling and lesions at the site of inoculation. The injection of highly virulent strains of non-O1 V. cholerae produced bacteremia. In contrast, only 1 of the 10 strains of O1 V. cholerae tested was highly lethal, and bacteremia was not detected. The highly virulent strains of O1 and non-O1 V. cholerae failed to produce keratoconjunctivitis in the Sereny test. A qualitative enzyme study showed no differences in ability to produce enzymes associated with invasiveness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1987.tb01988.x
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