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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 18 (1953), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 42 (1977), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Fresh pork shoulders were boned and ground through a 12.7 mm plate, packed in 1-kg packages, frozen at −28.9°C and stored at −17.8°C for 2 wk. The frozen pork samples were thawed for 24 hr at 3°C, then blended with a curing mixture consisting of 6 parts of NaCl and 1 part sugar at a rate of 47.5g/kg of meat with erythorbate and NaNO2 added to give levels of 0,183, 366 or 550 ppm of erythorbate and 0, 50,100, 150 or 200 ppm nitrite in the meat. The pork was then inoculated at the rate of 11 spores of Clostridium perfringens (UK92)/g, packed in quart jars (500/g jar) and cured for 2 wk at 1-4°C followed by 2 wk salt equalization at 12.8°C. Enumeration for C. perfringens began at this time and continued on alternate weeks during the 6-wk aging period. Recovery of C. perfringens spores was significantly reduced by NaNO2. After 4 wk, 80% of the inoculated spores were recovered from samples with no added nitrite while only about one-third of the spores were recovered from samples with the lowest level of NaNO2. Higher levels of nitrite and increased aging time reduced the number of spores recovered. At 10 wk, recovery was 38.0, 12.0, 5.4, 3.6 and 0.9% of inoculated spores, respectively, for 0, 50, 100, 150 and 200 ppm NaNO2. Sodium erythorbate had no significant effect on recovery of spores. Nitrite levels in the meat decreased rapidly with less than 60% of initial levels remaining after 24 hr. Only about 10% of initial concentrations were present after 4 wk. Results generally were similar for survival of C. perfringens spores and for nitrite losses when ground pork samples were dry-cured in stockinettes and aged for the same time and at the same temperature as those cured in jars.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 4 (1990), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: An 8kb segment of DNA from the 58/59 min region of the E. coli chromosome, which complements the defect of a mutant devoid of hydrogenase 3 activity, has been sequenced. Eight open reading frames were identified which are arranged in a transcriptional unit; all open reading frames were transcribed and translated in vivo in a T7 promoter/polymerase system. Analysis of the amino acid sequences derived from the nucleic acid sequences revealed that one of them, open reading frame 5 (0RF5), exhibits significant sequence similarity to conserved regions of the large subunit from Ni/Fe hydrogenases. Two of the open reading frames (orf2, orf6) code for proteins apparently carrying iron-sulphur clusters of the 4Fe/4S ferredoxin type. The product of one of the open reading frames, orf7, displays extensive sequence similarity with protein G from the chloroplast electron transport chain. ORF3 and ORF4, on the other hand, are extremely hydrophobic proteins with nine and six putative transmembrane helices, respectively. Over a limited hydrophilic sequence stretch, bordered by putative transmembrane areas, ORF3 and ORF4 exhibit homology with subunits 4 and 1 of mitochondrial and plastid NADH-ubiquinol oxidoreductases, respectively. The operon described, therefore, appears to comprise genes for redox carriers linking formate oxidation to proton reduction and for a hydrogenase of hitherto unique composition.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the American Water Resources Association 33 (1997), S. 0 
    ISSN: 1752-1688
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: : Constructed wetlands have recently gained popularity as an alternative method for wastewater treatment. This paper compares two design methodologies currently used for constructed wetlands; Tennessee Valley Authority (TVA) and the Environmental Protection Agency (EPA) methods. A discussion of parameters for both methods is given and a wetland treatment system is designed for an individual residence with typical BOD5 loads and flow rates.Calculation results revealed significant discrepancies in the required constructed wetlands volume, and thus detention time, stemming from inherent differences in the design methodologies. The EPA method relies heavily on plug flow kinetics, and is therefore sensitive to changes in the reaction rate constant and media porosity. Conversely, TVA determines the surface area by sizing in accordance with a recommended hydraulic loading criterion and is affected only by the hydraulic flow rates. This study concluded that a constructed wetland is a viable option under design considerations that are not favorable for traditional on-site wastewater treatment methods. However, it is recommended that conservative values for flow and loading rates be assumed to assure complete treatment for either of the design methods.
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  • 5
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. The eggs of 30 female chinook salmon, Oncorhynchus tshawvtscha (Walbaum), were collected at spawning. Some eggs from each fish were collected for bacteriologic study. Two salmon produced eggs judged to be of poor quality which were not used. The remaining 28 of the 30 groups of eggs were fertilized from a single sperm pool and the eggs incubated in separate groups. Mortality data on the developing salmon were recorded regularly through the twelfth week on feed. Unfertilized eggs from each group were surface-disinfected with an iodine solution, then crushed and subjected to a culture procedure designed to permit growth of as many bacterial types as possible. Bacteria were cultured and identified, and a comparison made of the types of organisms present in eggs from groups which later incurred high or low mortalities. Bacteria were recovered from both groups of salmon eggs. Although no single organism could be identified as a cause of increased mortality, the more frequent occurrence in the eggs of the ‘high mortality’ group of species of Vibrio, Listeria, Corynebacterium and Staphylococcus suggests that these bacteria may play a role. It is suggested that the cause of so-called early lifestage disease of salmon is multifactorial.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 92 (1994), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Seasonal variations of starch and soluble carbohydrates as well as extractable activities of enzymes involved in starch and maltose metabolism from 4-year-old twigs wood of Populus×Canadensis Moench ‘robusta’ were analysed during late winter, spring, and summer. A rapid decline of all enzymes occurred before or shortly after bud break in spring, accompanied by the complete degradation of starch and maltose. The inactivation proceded in a highly ordered sequence. Starch- and maltose-synthesizing enzymes. March phosphorylase (EC 2.4.1.1), and D-enzyme, (EC 2.4. 125) declined before hydrolysis of carbohydrates, while certain electrophoretic isoforms of amylases and maltose-degrading enzymes persisted until the storage compounds were digested. The enzymes of starch synthesis, ADP glucose pyrophosphorylase (EC 2.7.7.27) and starch synthase (EC 2.4.1.21). increased already in late spring while the activity of all other enzymes stayed on a very low level during summer. Comparison of enzyme activities and rate of renewed starch deposition in June showed that both steps, catalysed by ADP glucose pyrophosphorylase and starch synthase. may be rate limiting.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 44 (1979), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Microbial studies were conducted to determine the rate of build-up of surface bacteria during chilling and holding on carcasses of 141 lambs. Samples were obtained using the swab technique. Initial bacterial load was determined on hot carcasses. Chilled carcasses were sampled at 24 hr and after 4 and 7 days post slaughter. Bacterial counts indicated substantial reduction in numbers during chilling, at -2.2 °C for 24 hr from 6.4 × 103 to 5.1 × 102/cm2 for the total aerobic count on light lamb carcasses. Reduction in numbers of total aerobes during 24-hr chilling was not as great for heavy lamb carcasses, from 5.1 × 103 to 1.7 × 103/cm2 Numbers of phychrotrophic organisms after 4 and 7 days appear to be related to the thickness of fat cover. Carcasses having 0.36 cm or less of fat cover had significantly (P 〈 0.05) higher psychrophilic counts. No Salmonella were isolated and numbers of Clostridium perfringens in both groups of lamb carcasses were low.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 40 (1975), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 37 (1972), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 4 (1990), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The act gene of Escherichia coli encodes the pyruvate formate-lyase-activating enzyme which is necessary for the post-translational modification of pyruvate formate-lyase. The gene is located 191 bp downstream from the pfl structural gene. Northern blot analysis revealed that the act transcript is monocistronic and that transcription is independent of pfl gene expression. Through mapping of the 5′ and 3′ ends of the act transcript, sequences could be identified showing similarity to both an Escherichia coliσ70 promoter and to a rho-independent transcription terminator. Expression of the act gene was analysed with the aid of chromosomally integrated transcriptional and translational lacZ fusions. The results verified that the act gene is transcribed from its own promoter and that expression of the gene is essentially constitutive. Anaerobiosis led only to a two-fold increase in expression over that observed in aerobically grown cells and this elevated expression was independent of the transcriptional regulator, Fnr. Moreover, effectors such as pyruvate and nitrate, which substantially influence anaerobic transcription of the pfl gene, did not affect act gene expression.
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