ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
This experiment was carried out to confirm that the components of low-density lipoprotein (LDL) are not released from LDL during freezing and thawing. With the aid of ultrafiltration and gel filtration with Sephadex G 25, it was found that low molecular weight material was not liberated from frozen-thawed LDL. However, in centrifugation, a small amount of precipitate was found in unfrozen LDL and frozen-thawed LDL, and this amount was greater in the frozen-thawed LDL than in the unfrozen LDL. Specific gravities ranged from 1.02-1.04, and the phospholipid and protein contents of frozen-thawed and unfrozen LDL were higher than in the parent LDL. However, the SDS polyacrylamide gel electrophoretic patterns of the precipitates were the same as that of apo-LDL.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1980.tb07607.x
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