ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
We have analysed the effects of the lys 3a mutation on mRNA levels in the developing aleurone and starchy endosperm. The objective was to investigate whether the increase in non-hordein gene mRNA is a compensation for decreased hordein mRNA levels or if the increase is a result of a selective transcriptional or posttranscriptional regulation of specific genes. Our results show that protein Z and β-amylase which are downregulated by the lys 3a mutation are affected only in the starchy endosperm. In contrast, mRNA levels for the chymotrypsin inhibitors (CI-1, CI-2) are enhanced in the aleurone as well as in the starchy endosperm. The two isoform inhibitor genes of CI-1 (CI-1A, CI-1B) are differentially affected in the aleurone by the lys 3a mutation. The lys 3a gene enhances mRNA levels of two aleurone-specific genes. Together, these findings suggest that the lys 3a gene encodes or affects a regulatory factor which specifically modulates mRNA levels of several genes in different seed tissues.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1991.tb02143.x
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